Experiment: Ying_Nucleobase14 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3090 and GFF3091 are separated by 4 nucleotides GFF3091 and GFF3092 are separated by 2 nucleotides GFF3092 and GFF3093 are separated by 22 nucleotides
GFF3090 - DNA alkylation repair enzyme, at 167,825 to 168,976
GFF3090
GFF3091 - C-terminal domain of CinA type S; Protein Implicated in DNA repair function with RecA and MutS, at 168,981 to 169,463
GFF3091
GFF3092 - Phosphatidylglycerophosphatase A (EC 3.1.3.27), at 169,466 to 169,984
GFF3092
GFF3093 - Thiamine-monophosphate kinase (EC 2.7.4.16), at 170,007 to 170,978
GFF3093
Position (kb) 168
169
170 Strain fitness (log2 ratio) -2
-1
0
1 at 168.039 kb on + strand, within GFF3090 at 168.040 kb on - strand, within GFF3090 at 168.348 kb on + strand, within GFF3090 at 168.348 kb on + strand, within GFF3090 at 168.349 kb on - strand, within GFF3090 at 168.349 kb on - strand, within GFF3090 at 169.300 kb on + strand, within GFF3091 at 169.300 kb on + strand, within GFF3091 at 169.300 kb on + strand, within GFF3091 at 169.300 kb on + strand, within GFF3091 at 169.300 kb on + strand, within GFF3091 at 169.301 kb on - strand, within GFF3091 at 169.301 kb on - strand, within GFF3091 at 169.484 kb on + strand at 169.993 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2 remove 168,039 + GFF3090 0.19 +0.5 168,040 - GFF3090 0.19 -0.0 168,348 + GFF3090 0.45 +0.1 168,348 + GFF3090 0.45 -0.2 168,349 - GFF3090 0.45 +0.5 168,349 - GFF3090 0.45 -0.2 169,300 + GFF3091 0.66 -1.0 169,300 + GFF3091 0.66 -1.7 169,300 + GFF3091 0.66 -0.6 169,300 + GFF3091 0.66 -0.7 169,300 + GFF3091 0.66 -0.8 169,301 - GFF3091 0.66 -0.6 169,301 - GFF3091 0.66 -0.4 169,484 + -2.1 169,993 + -0.7
Or see this region's nucleotide sequence