Experiment: Ying_Nucleobase14 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF23 and GFF24 overlap by 4 nucleotides GFF24 and GFF25 are separated by 26 nucleotides GFF25 and GFF26 are separated by 119 nucleotides GFF26 and GFF27 are separated by 104 nucleotides
GFF23 - ABC transporter permease protein with unknown substrate, at 21,077 to 21,865
GFF23
GFF24 - Hydroxymethylpyrimidine ABC transporter, transmembrane component, at 21,862 to 22,620
GFF24
GFF25 - putative monooxygenase, at 22,647 to 24,191
GFF25
GFF26 - hypothetical protein, at 24,311 to 25,072
GFF26
GFF27 - Pca regulon regulatory protein PcaR, at 25,177 to 25,941
GFF27
Position (kb) 22
23
24
25 Strain fitness (log2 ratio) -2
-1
0
1 at 21.786 kb on - strand, within GFF23 at 22.471 kb on + strand, within GFF24 at 22.471 kb on + strand, within GFF24 at 22.646 kb on + strand at 22.917 kb on + strand, within GFF25 at 22.918 kb on - strand, within GFF25 at 23.142 kb on + strand, within GFF25 at 23.143 kb on - strand, within GFF25 at 23.143 kb on - strand, within GFF25 at 23.193 kb on - strand, within GFF25 at 23.293 kb on - strand, within GFF25 at 23.472 kb on + strand, within GFF25 at 23.472 kb on + strand, within GFF25 at 23.472 kb on + strand, within GFF25 at 23.472 kb on + strand, within GFF25 at 23.472 kb on + strand, within GFF25 at 23.473 kb on - strand, within GFF25 at 23.520 kb on + strand, within GFF25 at 23.598 kb on + strand, within GFF25 at 23.599 kb on - strand, within GFF25 at 23.599 kb on - strand, within GFF25 at 23.619 kb on + strand, within GFF25 at 23.619 kb on + strand, within GFF25 at 23.662 kb on - strand, within GFF25 at 23.682 kb on + strand, within GFF25 at 23.683 kb on - strand, within GFF25 at 23.911 kb on - strand, within GFF25 at 23.946 kb on - strand, within GFF25 at 23.979 kb on + strand, within GFF25 at 23.979 kb on + strand, within GFF25 at 23.979 kb on + strand, within GFF25 at 23.979 kb on + strand, within GFF25 at 23.980 kb on - strand, within GFF25 at 23.980 kb on - strand, within GFF25 at 23.980 kb on - strand, within GFF25 at 23.980 kb on - strand, within GFF25 at 24.165 kb on + strand at 24.491 kb on + strand, within GFF26 at 24.927 kb on - strand, within GFF26 at 24.927 kb on - strand, within GFF26 at 24.927 kb on - strand, within GFF26 at 25.057 kb on + strand at 25.057 kb on + strand at 25.057 kb on + strand at 25.057 kb on + strand at 25.058 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2 remove 21,786 - GFF23 0.90 -0.1 22,471 + GFF24 0.80 +0.0 22,471 + GFF24 0.80 -0.1 22,646 + -0.1 22,917 + GFF25 0.17 -0.5 22,918 - GFF25 0.18 -0.5 23,142 + GFF25 0.32 -0.8 23,143 - GFF25 0.32 +0.5 23,143 - GFF25 0.32 -0.3 23,193 - GFF25 0.35 -0.5 23,293 - GFF25 0.42 -1.4 23,472 + GFF25 0.53 -0.2 23,472 + GFF25 0.53 +0.6 23,472 + GFF25 0.53 -0.3 23,472 + GFF25 0.53 +0.3 23,472 + GFF25 0.53 +0.0 23,473 - GFF25 0.53 +0.5 23,520 + GFF25 0.57 +0.9 23,598 + GFF25 0.62 -0.2 23,599 - GFF25 0.62 -0.2 23,599 - GFF25 0.62 -0.1 23,619 + GFF25 0.63 +0.2 23,619 + GFF25 0.63 -0.2 23,662 - GFF25 0.66 +0.1 23,682 + GFF25 0.67 -0.7 23,683 - GFF25 0.67 -0.6 23,911 - GFF25 0.82 -0.6 23,946 - GFF25 0.84 -0.8 23,979 + GFF25 0.86 +1.7 23,979 + GFF25 0.86 -0.3 23,979 + GFF25 0.86 -0.6 23,979 + GFF25 0.86 -1.3 23,980 - GFF25 0.86 +0.9 23,980 - GFF25 0.86 +0.0 23,980 - GFF25 0.86 +0.8 23,980 - GFF25 0.86 -0.4 24,165 + -0.9 24,491 + GFF26 0.24 -1.8 24,927 - GFF26 0.81 -0.1 24,927 - GFF26 0.81 +0.3 24,927 - GFF26 0.81 -0.2 25,057 + -0.9 25,057 + -0.5 25,057 + -0.4 25,057 + +0.1 25,058 - -0.5
Or see this region's nucleotide sequence