Experiment: Ying_Nucleobase14 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2424 and GFF2425 are separated by 66 nucleotides GFF2425 and GFF2426 are separated by 122 nucleotides GFF2426 and GFF2427 are separated by 30 nucleotides
GFF2424 - Succinate dehydrogenase/fumarate reductase, flavoprotein subunit, at 3,071 to 4,816
GFF2424
GFF2425 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 4,883 to 5,626
GFF2425
GFF2426 - Sugar phosphate isomerases/epimerases, at 5,749 to 6,501
GFF2426
GFF2427 - TRAP-type C4-dicarboxylate transport system, periplasmic component, at 6,532 to 7,566
GFF2427
Position (kb) 5
6
7 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4.754 kb on + strand at 4.755 kb on - strand at 4.828 kb on - strand at 4.828 kb on - strand at 5.183 kb on + strand, within GFF2425 at 5.183 kb on + strand, within GFF2425 at 5.184 kb on - strand, within GFF2425 at 5.184 kb on - strand, within GFF2425 at 5.235 kb on + strand, within GFF2425 at 5.342 kb on + strand, within GFF2425 at 5.342 kb on + strand, within GFF2425 at 5.354 kb on - strand, within GFF2425 at 5.621 kb on + strand at 5.621 kb on + strand at 5.622 kb on - strand at 5.622 kb on - strand at 5.622 kb on - strand at 6.075 kb on - strand, within GFF2426 at 6.106 kb on + strand, within GFF2426 at 6.106 kb on + strand, within GFF2426 at 6.106 kb on + strand, within GFF2426 at 6.106 kb on + strand, within GFF2426 at 6.107 kb on - strand, within GFF2426 at 6.159 kb on + strand, within GFF2426 at 6.160 kb on - strand, within GFF2426 at 6.238 kb on + strand, within GFF2426 at 6.239 kb on - strand, within GFF2426 at 6.535 kb on + strand at 6.536 kb on - strand at 6.536 kb on - strand at 6.536 kb on - strand at 6.536 kb on - strand at 6.536 kb on - strand at 6.536 kb on - strand at 6.536 kb on - strand at 6.547 kb on + strand at 6.547 kb on + strand at 6.548 kb on - strand at 6.548 kb on - strand at 6.548 kb on - strand at 6.661 kb on + strand, within GFF2427 at 6.661 kb on + strand, within GFF2427 at 6.813 kb on + strand, within GFF2427 at 6.985 kb on - strand, within GFF2427 at 7.093 kb on + strand, within GFF2427 at 7.093 kb on + strand, within GFF2427 at 7.093 kb on + strand, within GFF2427 at 7.093 kb on + strand, within GFF2427 at 7.094 kb on - strand, within GFF2427 at 7.153 kb on + strand, within GFF2427 at 7.154 kb on - strand, within GFF2427 at 7.226 kb on - strand, within GFF2427 at 7.240 kb on + strand, within GFF2427 at 7.240 kb on + strand, within GFF2427 at 7.240 kb on + strand, within GFF2427 at 7.240 kb on + strand, within GFF2427 at 7.240 kb on + strand, within GFF2427 at 7.240 kb on + strand, within GFF2427 at 7.240 kb on + strand, within GFF2427 at 7.241 kb on - strand, within GFF2427 at 7.241 kb on - strand, within GFF2427 at 7.241 kb on - strand, within GFF2427 at 7.241 kb on - strand, within GFF2427 at 7.264 kb on + strand, within GFF2427 at 7.264 kb on + strand, within GFF2427 at 7.264 kb on + strand, within GFF2427 at 7.264 kb on + strand, within GFF2427 at 7.264 kb on + strand, within GFF2427 at 7.264 kb on + strand, within GFF2427 at 7.264 kb on + strand, within GFF2427 at 7.264 kb on + strand, within GFF2427 at 7.265 kb on - strand, within GFF2427 at 7.265 kb on - strand, within GFF2427 at 7.265 kb on - strand, within GFF2427 at 7.265 kb on - strand, within GFF2427 at 7.265 kb on - strand, within GFF2427 at 7.265 kb on - strand, within GFF2427 at 7.265 kb on - strand, within GFF2427 at 7.498 kb on + strand at 7.498 kb on + strand at 7.498 kb on + strand at 7.499 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2 remove 4,754 + +0.4 4,755 - +0.6 4,828 - -0.5 4,828 - -0.5 5,183 + GFF2425 0.40 +0.0 5,183 + GFF2425 0.40 -0.2 5,184 - GFF2425 0.40 -0.3 5,184 - GFF2425 0.40 -0.2 5,235 + GFF2425 0.47 +1.1 5,342 + GFF2425 0.62 +0.1 5,342 + GFF2425 0.62 -0.5 5,354 - GFF2425 0.63 -0.0 5,621 + +0.2 5,621 + -0.5 5,622 - +0.2 5,622 - -1.7 5,622 - -0.7 6,075 - GFF2426 0.43 -0.8 6,106 + GFF2426 0.47 -0.4 6,106 + GFF2426 0.47 -0.0 6,106 + GFF2426 0.47 -0.0 6,106 + GFF2426 0.47 +0.2 6,107 - GFF2426 0.48 -1.4 6,159 + GFF2426 0.54 +0.5 6,160 - GFF2426 0.55 +0.0 6,238 + GFF2426 0.65 -2.1 6,239 - GFF2426 0.65 +1.0 6,535 + +0.0 6,536 - -0.7 6,536 - +0.2 6,536 - +0.3 6,536 - -0.5 6,536 - +0.3 6,536 - +0.2 6,536 - +0.2 6,547 + -0.1 6,547 + +2.0 6,548 - -0.1 6,548 - +0.5 6,548 - -1.7 6,661 + GFF2427 0.12 +0.5 6,661 + GFF2427 0.12 -0.4 6,813 + GFF2427 0.27 -2.1 6,985 - GFF2427 0.44 -0.1 7,093 + GFF2427 0.54 +0.1 7,093 + GFF2427 0.54 +0.0 7,093 + GFF2427 0.54 +0.4 7,093 + GFF2427 0.54 +0.3 7,094 - GFF2427 0.54 -0.4 7,153 + GFF2427 0.60 +0.3 7,154 - GFF2427 0.60 -0.6 7,226 - GFF2427 0.67 +1.5 7,240 + GFF2427 0.68 +0.5 7,240 + GFF2427 0.68 -0.1 7,240 + GFF2427 0.68 -0.5 7,240 + GFF2427 0.68 -0.4 7,240 + GFF2427 0.68 +0.3 7,240 + GFF2427 0.68 +0.2 7,240 + GFF2427 0.68 -0.1 7,241 - GFF2427 0.69 +0.9 7,241 - GFF2427 0.69 +0.5 7,241 - GFF2427 0.69 -0.3 7,241 - GFF2427 0.69 +0.2 7,264 + GFF2427 0.71 -0.1 7,264 + GFF2427 0.71 -0.0 7,264 + GFF2427 0.71 -0.0 7,264 + GFF2427 0.71 +0.1 7,264 + GFF2427 0.71 +0.1 7,264 + GFF2427 0.71 -0.4 7,264 + GFF2427 0.71 -0.5 7,264 + GFF2427 0.71 -0.2 7,265 - GFF2427 0.71 -0.5 7,265 - GFF2427 0.71 -0.4 7,265 - GFF2427 0.71 +0.5 7,265 - GFF2427 0.71 +1.1 7,265 - GFF2427 0.71 -0.1 7,265 - GFF2427 0.71 +0.1 7,265 - GFF2427 0.71 +0.2 7,498 + +1.1 7,498 + +0.5 7,498 + -0.0 7,499 - +0.1
Or see this region's nucleotide sequence