Experiment: Ying_Nucleobase14 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF185 and GFF186 are separated by 40 nucleotides GFF186 and GFF187 overlap by 4 nucleotides GFF187 and GFF188 are separated by 13 nucleotides GFF188 and GFF189 are separated by 48 nucleotides
GFF185 - Oligopeptide transport system permease protein OppC (TC 3.A.1.5.1), at 197,928 to 198,857
GFF185
GFF186 - hypothetical protein, at 198,898 to 199,665
GFF186
GFF187 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 199,662 to 200,624
GFF187
GFF188 - probable extracellular solute-binding protein, at 200,638 to 201,387
GFF188
GFF189 - MFS permease, at 201,436 to 202,833
GFF189
Position (kb) 199
200
201 Strain fitness (log2 ratio) -1
0
1 at 199.180 kb on + strand, within GFF186 at 199.180 kb on + strand, within GFF186 at 199.225 kb on + strand, within GFF186 at 199.225 kb on + strand, within GFF186 at 199.225 kb on + strand, within GFF186 at 199.225 kb on + strand, within GFF186 at 199.226 kb on - strand, within GFF186 at 199.246 kb on + strand, within GFF186 at 199.426 kb on + strand, within GFF186 at 199.687 kb on - strand at 200.149 kb on - strand, within GFF187 at 200.149 kb on - strand, within GFF187 at 200.987 kb on + strand, within GFF188 at 200.987 kb on + strand, within GFF188 at 200.988 kb on - strand, within GFF188 at 200.988 kb on - strand, within GFF188 at 201.074 kb on + strand, within GFF188 at 201.074 kb on + strand, within GFF188 at 201.075 kb on - strand, within GFF188 at 201.095 kb on + strand, within GFF188 at 201.095 kb on + strand, within GFF188 at 201.533 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2 remove 199,180 + GFF186 0.37 +0.3 199,180 + GFF186 0.37 -0.3 199,225 + GFF186 0.43 -1.4 199,225 + GFF186 0.43 +1.2 199,225 + GFF186 0.43 +0.2 199,225 + GFF186 0.43 -0.5 199,226 - GFF186 0.43 -0.3 199,246 + GFF186 0.45 -1.1 199,426 + GFF186 0.69 +0.1 199,687 - -0.3 200,149 - GFF187 0.51 -0.3 200,149 - GFF187 0.51 -0.2 200,987 + GFF188 0.47 -0.1 200,987 + GFF188 0.47 -0.2 200,988 - GFF188 0.47 -0.4 200,988 - GFF188 0.47 -1.1 201,074 + GFF188 0.58 -0.5 201,074 + GFF188 0.58 -0.6 201,075 - GFF188 0.58 -0.8 201,095 + GFF188 0.61 +1.2 201,095 + GFF188 0.61 +0.9 201,533 + -0.9
Or see this region's nucleotide sequence