Experiment: Ying_Nucleobase14 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF158 and GFF159 overlap by 1 nucleotides GFF159 and GFF160 are separated by 41 nucleotides GFF160 and GFF161 overlap by 4 nucleotides
GFF158 - NAD synthetase (EC 6.3.1.5), at 171,731 to 172,702
GFF158
GFF159 - Acyl carrier protein, at 172,702 to 172,956
GFF159
GFF160 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 172,998 to 174,515
GFF160
GFF161 - Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4), at 174,512 to 176,452
GFF161
Position (kb) 172
173
174
175 Strain fitness (log2 ratio) -2
-1
0
1 at 172.030 kb on - strand, within GFF158 at 172.030 kb on - strand, within GFF158 at 172.096 kb on - strand, within GFF158 at 172.128 kb on + strand, within GFF158 at 172.128 kb on + strand, within GFF158 at 172.128 kb on + strand, within GFF158 at 172.150 kb on - strand, within GFF158 at 172.152 kb on + strand, within GFF158 at 172.152 kb on + strand, within GFF158 at 172.152 kb on + strand, within GFF158 at 172.210 kb on - strand, within GFF158 at 172.330 kb on - strand, within GFF158 at 172.335 kb on + strand, within GFF158 at 172.335 kb on + strand, within GFF158 at 172.378 kb on - strand, within GFF158 at 173.309 kb on - strand, within GFF160 at 173.420 kb on - strand, within GFF160 at 173.602 kb on + strand, within GFF160 at 173.603 kb on - strand, within GFF160 at 173.603 kb on - strand, within GFF160 at 173.603 kb on - strand, within GFF160 at 173.603 kb on - strand, within GFF160 at 173.861 kb on + strand, within GFF160 at 173.861 kb on + strand, within GFF160 at 173.938 kb on + strand, within GFF160 at 173.938 kb on + strand, within GFF160 at 174.023 kb on - strand, within GFF160 at 174.298 kb on + strand, within GFF160 at 174.969 kb on + strand, within GFF161 at 175.000 kb on - strand, within GFF161 at 175.000 kb on - strand, within GFF161 at 175.332 kb on + strand, within GFF161
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2 remove 172,030 - GFF158 0.31 -0.6 172,030 - GFF158 0.31 +0.1 172,096 - GFF158 0.38 +0.5 172,128 + GFF158 0.41 -0.2 172,128 + GFF158 0.41 +0.5 172,128 + GFF158 0.41 -0.1 172,150 - GFF158 0.43 +0.4 172,152 + GFF158 0.43 -0.9 172,152 + GFF158 0.43 +0.5 172,152 + GFF158 0.43 -0.6 172,210 - GFF158 0.49 +0.3 172,330 - GFF158 0.62 -0.4 172,335 + GFF158 0.62 -0.0 172,335 + GFF158 0.62 +0.1 172,378 - GFF158 0.67 -0.0 173,309 - GFF160 0.20 -0.5 173,420 - GFF160 0.28 -0.7 173,602 + GFF160 0.40 -0.2 173,603 - GFF160 0.40 -0.5 173,603 - GFF160 0.40 +0.2 173,603 - GFF160 0.40 -0.4 173,603 - GFF160 0.40 +0.1 173,861 + GFF160 0.57 -0.1 173,861 + GFF160 0.57 -2.4 173,938 + GFF160 0.62 -0.8 173,938 + GFF160 0.62 +0.3 174,023 - GFF160 0.68 -0.1 174,298 + GFF160 0.86 -0.0 174,969 + GFF161 0.24 +0.3 175,000 - GFF161 0.25 -1.4 175,000 - GFF161 0.25 -2.0 175,332 + GFF161 0.42 -0.3
Or see this region's nucleotide sequence