Experiment: Ying_Nucleobase14 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1495 and GFF1496 are separated by 2 nucleotides GFF1496 and GFF1497 are separated by 17 nucleotides GFF1497 and GFF1498 are separated by 47 nucleotides
GFF1495 - Tricarboxylate transport protein TctC, at 344,401 to 345,387
GFF1495
GFF1496 - beta-lactamase domain protein, at 345,390 to 346,355
GFF1496
GFF1497 - 2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60), at 346,373 to 347,257
GFF1497
GFF1498 - 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.n4), at 347,305 to 348,093
GFF1498
Position (kb)
345
346
347 Strain fitness (log2 ratio)
-1
0
1 at 344.878 kb on + strand, within GFF1495 at 344.879 kb on - strand, within GFF1495 at 345.675 kb on + strand, within GFF1496 at 346.077 kb on + strand, within GFF1496 at 346.159 kb on - strand, within GFF1496 at 346.917 kb on + strand, within GFF1497 at 346.917 kb on + strand, within GFF1497 at 347.178 kb on + strand at 347.178 kb on + strand at 347.178 kb on + strand at 347.178 kb on + strand at 347.179 kb on - strand at 347.179 kb on - strand at 347.179 kb on - strand at 347.277 kb on + strand at 347.277 kb on + strand at 347.277 kb on + strand at 347.287 kb on + strand at 347.287 kb on + strand at 347.287 kb on + strand at 347.287 kb on + strand at 347.288 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2 remove 344,878 + GFF1495 0.48 -0.8 344,879 - GFF1495 0.48 -0.1 345,675 + GFF1496 0.30 -1.6 346,077 + GFF1496 0.71 -0.4 346,159 - GFF1496 0.80 -0.3 346,917 + GFF1497 0.61 -0.4 346,917 + GFF1497 0.61 +0.1 347,178 + +0.2 347,178 + +0.2 347,178 + +0.1 347,178 + +0.3 347,179 - +0.6 347,179 - +0.4 347,179 - +0.4 347,277 + +0.1 347,277 + -0.4 347,277 + -0.1 347,287 + -0.6 347,287 + +0.1 347,287 + -0.2 347,287 + -0.1 347,288 - +0.3
Or see this region's nucleotide sequence