Experiment: Ying_Nucleobase14 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1454 and GFF1455 overlap by 4 nucleotides GFF1455 and GFF1456 are separated by 28 nucleotides GFF1456 and GFF1457 are separated by 116 nucleotides GFF1457 and GFF1458 are separated by 15 nucleotides
GFF1454 - Flagellum-specific ATP synthase FliI, at 308,491 to 309,969
GFF1454
GFF1455 - Flagellar protein FliJ, at 309,966 to 310,433
GFF1455
GFF1456 - Flagellar hook-length control protein FliK, at 310,462 to 311,733
GFF1456
GFF1457 - Flagellar biosynthesis protein FliL, at 311,850 to 312,419
GFF1457
GFF1458 - Flagellar motor switch protein FliM, at 312,435 to 313,439
GFF1458
Position (kb) 310
311
312 Strain fitness (log2 ratio) -1
0
1
2 at 309.508 kb on + strand, within GFF1454 at 309.508 kb on + strand, within GFF1454 at 309.508 kb on + strand, within GFF1454 at 309.805 kb on + strand, within GFF1454 at 309.950 kb on - strand at 310.098 kb on + strand, within GFF1455 at 311.745 kb on - strand at 311.745 kb on - strand at 311.764 kb on + strand at 311.764 kb on + strand at 311.765 kb on - strand at 311.765 kb on - strand at 311.797 kb on + strand at 311.819 kb on + strand at 312.010 kb on - strand, within GFF1457 at 312.187 kb on - strand, within GFF1457 at 312.316 kb on - strand, within GFF1457 at 312.547 kb on - strand, within GFF1458 at 312.547 kb on - strand, within GFF1458 at 312.547 kb on - strand, within GFF1458 at 312.699 kb on + strand, within GFF1458 at 312.700 kb on - strand, within GFF1458 at 312.700 kb on - strand, within GFF1458 at 312.700 kb on - strand, within GFF1458
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2 remove 309,508 + GFF1454 0.69 +1.8 309,508 + GFF1454 0.69 -0.2 309,508 + GFF1454 0.69 -0.1 309,805 + GFF1454 0.89 +0.1 309,950 - -0.5 310,098 + GFF1455 0.28 -0.1 311,745 - +0.1 311,745 - +0.5 311,764 + -1.6 311,764 + +0.8 311,765 - +0.8 311,765 - -0.3 311,797 + -1.1 311,819 + +0.9 312,010 - GFF1457 0.28 +0.7 312,187 - GFF1457 0.59 +0.1 312,316 - GFF1457 0.82 +0.1 312,547 - GFF1458 0.11 +0.7 312,547 - GFF1458 0.11 -0.3 312,547 - GFF1458 0.11 -0.9 312,699 + GFF1458 0.26 +1.2 312,700 - GFF1458 0.26 -0.4 312,700 - GFF1458 0.26 +0.1 312,700 - GFF1458 0.26 +0.7
Or see this region's nucleotide sequence