Experiment: Ying_Nucleobase14 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1216 and GFF1217 are separated by 26 nucleotides GFF1217 and GFF1218 are separated by 99 nucleotides GFF1218 and GFF1219 are separated by 20 nucleotides
GFF1216 - Quinolinate synthetase (EC 2.5.1.72), at 81,157 to 82,269
GFF1216
GFF1217 - Quinolinate phosphoribosyltransferase [decarboxylating] (EC 2.4.2.19), at 82,296 to 83,159
GFF1217
GFF1218 - 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11), at 83,259 to 84,119
GFF1218
GFF1219 - putative; ORF located using Glimmer/Genemark, at 84,140 to 84,865
GFF1219
Position (kb) 82
83
84 Strain fitness (log2 ratio) -1
0
1
2 at 81.687 kb on - strand, within GFF1216 at 81.687 kb on - strand, within GFF1216 at 81.687 kb on - strand, within GFF1216 at 81.687 kb on - strand, within GFF1216 at 81.821 kb on + strand, within GFF1216 at 81.821 kb on + strand, within GFF1216 at 81.822 kb on - strand, within GFF1216 at 82.091 kb on + strand, within GFF1216 at 82.091 kb on + strand, within GFF1216 at 82.091 kb on + strand, within GFF1216 at 82.091 kb on + strand, within GFF1216 at 82.091 kb on + strand, within GFF1216 at 82.091 kb on + strand, within GFF1216 at 82.091 kb on + strand, within GFF1216 at 82.091 kb on + strand, within GFF1216 at 82.091 kb on + strand, within GFF1216 at 82.091 kb on + strand, within GFF1216 at 82.092 kb on - strand, within GFF1216 at 82.092 kb on - strand, within GFF1216 at 82.092 kb on - strand, within GFF1216 at 82.092 kb on - strand, within GFF1216 at 82.092 kb on - strand, within GFF1216 at 82.092 kb on - strand, within GFF1216 at 82.092 kb on - strand, within GFF1216 at 82.092 kb on - strand, within GFF1216 at 82.092 kb on - strand, within GFF1216 at 82.094 kb on + strand, within GFF1216 at 82.095 kb on - strand, within GFF1216 at 82.095 kb on - strand, within GFF1216 at 82.229 kb on + strand at 82.230 kb on - strand at 82.263 kb on + strand at 82.263 kb on + strand at 82.264 kb on - strand at 82.455 kb on + strand, within GFF1217 at 82.456 kb on - strand, within GFF1217 at 82.456 kb on - strand, within GFF1217 at 82.456 kb on - strand, within GFF1217 at 82.661 kb on - strand, within GFF1217 at 82.705 kb on + strand, within GFF1217 at 82.706 kb on - strand, within GFF1217 at 82.706 kb on - strand, within GFF1217 at 83.185 kb on + strand at 83.185 kb on + strand at 83.186 kb on - strand at 83.186 kb on - strand at 83.187 kb on + strand at 83.187 kb on + strand at 83.187 kb on + strand at 83.187 kb on + strand at 83.187 kb on + strand at 83.187 kb on + strand at 83.187 kb on + strand at 83.188 kb on - strand at 83.188 kb on - strand at 83.188 kb on - strand at 83.188 kb on - strand at 83.188 kb on - strand at 83.188 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2 remove 81,687 - GFF1216 0.48 +0.3 81,687 - GFF1216 0.48 +0.5 81,687 - GFF1216 0.48 -0.5 81,687 - GFF1216 0.48 +0.4 81,821 + GFF1216 0.60 +0.3 81,821 + GFF1216 0.60 -0.1 81,822 - GFF1216 0.60 +1.2 82,091 + GFF1216 0.84 +0.2 82,091 + GFF1216 0.84 +0.3 82,091 + GFF1216 0.84 +0.4 82,091 + GFF1216 0.84 +0.1 82,091 + GFF1216 0.84 +0.1 82,091 + GFF1216 0.84 +0.2 82,091 + GFF1216 0.84 +0.4 82,091 + GFF1216 0.84 -0.6 82,091 + GFF1216 0.84 +0.2 82,091 + GFF1216 0.84 +0.4 82,092 - GFF1216 0.84 +0.9 82,092 - GFF1216 0.84 -0.1 82,092 - GFF1216 0.84 +0.7 82,092 - GFF1216 0.84 +0.2 82,092 - GFF1216 0.84 +0.0 82,092 - GFF1216 0.84 +0.9 82,092 - GFF1216 0.84 +0.8 82,092 - GFF1216 0.84 -0.5 82,092 - GFF1216 0.84 +0.2 82,094 + GFF1216 0.84 +0.6 82,095 - GFF1216 0.84 +0.6 82,095 - GFF1216 0.84 -0.6 82,229 + +0.5 82,230 - -0.5 82,263 + +0.5 82,263 + +2.1 82,264 - +1.2 82,455 + GFF1217 0.18 -0.4 82,456 - GFF1217 0.19 +0.1 82,456 - GFF1217 0.19 +0.8 82,456 - GFF1217 0.19 +1.7 82,661 - GFF1217 0.42 +0.0 82,705 + GFF1217 0.47 -0.9 82,706 - GFF1217 0.47 -0.1 82,706 - GFF1217 0.47 +0.3 83,185 + +0.3 83,185 + +0.6 83,186 - +0.4 83,186 - +0.3 83,187 + -0.3 83,187 + -0.6 83,187 + -0.6 83,187 + -1.0 83,187 + -0.2 83,187 + -0.4 83,187 + +1.0 83,188 - +0.7 83,188 - -0.4 83,188 - -1.2 83,188 - -0.6 83,188 - -0.2 83,188 - +0.1
Or see this region's nucleotide sequence