Experiment: Supernatant; Pseudomonas DC3000 grown in LB; 0.5 X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt apaG and COO64_RS22520 overlap by 1 nucleotides COO64_RS22520 and glpE are separated by 14 nucleotides glpE and COO64_RS22530 are separated by 281 nucleotides
COO64_RS22515: apaG - Co2+/Mg2+ efflux protein ApaG, at 38,849 to 39,229
apaG
COO64_RS22520: COO64_RS22520 - symmetrical bis(5'-nucleosyl)-tetraphosphatase, at 39,229 to 40,104
_RS22520
COO64_RS22525: glpE - thiosulfate sulfurtransferase GlpE, at 40,119 to 40,448
glpE
COO64_RS22530: COO64_RS22530 - PrkA family serine protein kinase, at 40,730 to 42,652
_RS22530
Position (kb) 40
41 Strain fitness (log2 ratio) -2
-1
0
1 at 39.146 kb on + strand, within apaG at 39.146 kb on + strand, within apaG at 39.147 kb on - strand, within apaG at 39.169 kb on + strand, within apaG at 39.238 kb on + strand at 39.683 kb on - strand, within COO64_RS22520 at 40.083 kb on + strand at 40.084 kb on - strand at 40.209 kb on + strand, within glpE at 40.209 kb on + strand, within glpE at 40.210 kb on - strand, within glpE at 40.210 kb on - strand, within glpE at 40.210 kb on - strand, within glpE at 40.210 kb on - strand, within glpE at 40.210 kb on - strand, within glpE at 40.210 kb on - strand, within glpE at 40.210 kb on - strand, within glpE at 40.210 kb on - strand, within glpE at 40.311 kb on + strand, within glpE at 40.312 kb on - strand, within glpE at 40.344 kb on + strand, within glpE at 40.344 kb on + strand, within glpE at 40.345 kb on - strand, within glpE at 40.374 kb on + strand, within glpE at 40.375 kb on - strand, within glpE at 40.375 kb on - strand, within glpE at 40.375 kb on - strand, within glpE at 40.414 kb on - strand, within glpE at 40.525 kb on + strand at 40.525 kb on + strand at 40.525 kb on + strand at 40.525 kb on + strand at 40.525 kb on + strand at 40.526 kb on - strand at 40.526 kb on - strand at 40.550 kb on + strand at 40.557 kb on + strand at 40.558 kb on - strand at 40.558 kb on - strand at 40.558 kb on - strand at 40.558 kb on - strand at 40.558 kb on - strand at 40.564 kb on - strand at 40.564 kb on - strand at 40.596 kb on + strand at 40.596 kb on + strand at 40.629 kb on + strand at 40.629 kb on + strand at 40.629 kb on + strand at 40.630 kb on - strand at 40.630 kb on - strand at 40.630 kb on - strand at 40.630 kb on - strand at 40.645 kb on + strand at 40.645 kb on + strand at 40.647 kb on + strand at 40.647 kb on + strand at 40.647 kb on + strand at 40.647 kb on + strand at 40.647 kb on + strand at 40.647 kb on + strand at 40.647 kb on + strand at 40.647 kb on + strand at 40.647 kb on + strand at 40.647 kb on + strand at 40.648 kb on - strand at 40.648 kb on - strand at 40.648 kb on - strand at 40.648 kb on - strand at 40.648 kb on - strand at 40.648 kb on - strand at 40.648 kb on - strand at 40.648 kb on - strand at 40.648 kb on - strand at 40.648 kb on - strand at 40.648 kb on - strand at 40.648 kb on - strand at 40.648 kb on - strand at 40.648 kb on - strand at 40.648 kb on - strand at 40.648 kb on - strand at 40.648 kb on - strand at 40.711 kb on + strand at 40.735 kb on + strand at 40.735 kb on + strand at 40.736 kb on - strand at 40.736 kb on - strand at 40.769 kb on - strand at 40.799 kb on + strand at 40.800 kb on - strand at 40.800 kb on - strand at 40.832 kb on + strand at 40.832 kb on + strand at 40.832 kb on + strand at 40.832 kb on + strand at 40.833 kb on - strand at 40.833 kb on - strand at 41.000 kb on + strand, within COO64_RS22530 at 41.000 kb on + strand, within COO64_RS22530 at 41.000 kb on + strand, within COO64_RS22530 at 41.000 kb on + strand, within COO64_RS22530 at 41.048 kb on + strand, within COO64_RS22530 at 41.049 kb on - strand, within COO64_RS22530 at 41.055 kb on + strand, within COO64_RS22530 at 41.056 kb on - strand, within COO64_RS22530 at 41.056 kb on - strand, within COO64_RS22530 at 41.056 kb on - strand, within COO64_RS22530 at 41.074 kb on + strand, within COO64_RS22530 at 41.126 kb on + strand, within COO64_RS22530 at 41.126 kb on + strand, within COO64_RS22530 at 41.126 kb on + strand, within COO64_RS22530 at 41.126 kb on + strand, within COO64_RS22530 at 41.126 kb on + strand, within COO64_RS22530 at 41.126 kb on + strand, within COO64_RS22530 at 41.126 kb on + strand, within COO64_RS22530 at 41.126 kb on + strand, within COO64_RS22530 at 41.126 kb on + strand, within COO64_RS22530 at 41.126 kb on + strand, within COO64_RS22530 at 41.126 kb on + strand, within COO64_RS22530 at 41.127 kb on - strand, within COO64_RS22530 at 41.127 kb on - strand, within COO64_RS22530 at 41.127 kb on - strand, within COO64_RS22530 at 41.127 kb on - strand, within COO64_RS22530 at 41.127 kb on - strand, within COO64_RS22530 at 41.127 kb on - strand, within COO64_RS22530 at 41.127 kb on - strand, within COO64_RS22530 at 41.127 kb on - strand, within COO64_RS22530 at 41.127 kb on - strand, within COO64_RS22530 at 41.127 kb on - strand, within COO64_RS22530 at 41.127 kb on - strand, within COO64_RS22530 at 41.127 kb on - strand, within COO64_RS22530 at 41.228 kb on + strand, within COO64_RS22530 at 41.316 kb on - strand, within COO64_RS22530 at 41.380 kb on + strand, within COO64_RS22530 at 41.380 kb on + strand, within COO64_RS22530 at 41.380 kb on + strand, within COO64_RS22530 at 41.381 kb on - strand, within COO64_RS22530 at 41.426 kb on + strand, within COO64_RS22530 at 41.426 kb on + strand, within COO64_RS22530 at 41.426 kb on + strand, within COO64_RS22530 at 41.427 kb on - strand, within COO64_RS22530
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Pseudomonas DC3000 grown in LB; 0.5 X remove 39,146 + apaG COO64_RS22515 0.78 -0.3 39,146 + apaG COO64_RS22515 0.78 -0.3 39,147 - apaG COO64_RS22515 0.78 -1.5 39,169 + apaG COO64_RS22515 0.84 +0.6 39,238 + -0.6 39,683 - COO64_RS22520 0.52 +0.3 40,083 + -0.4 40,084 - +0.2 40,209 + glpE COO64_RS22525 0.27 +0.5 40,209 + glpE COO64_RS22525 0.27 +1.0 40,210 - glpE COO64_RS22525 0.28 -0.8 40,210 - glpE COO64_RS22525 0.28 +0.1 40,210 - glpE COO64_RS22525 0.28 -1.4 40,210 - glpE COO64_RS22525 0.28 +0.1 40,210 - glpE COO64_RS22525 0.28 +0.0 40,210 - glpE COO64_RS22525 0.28 -0.2 40,210 - glpE COO64_RS22525 0.28 +1.5 40,210 - glpE COO64_RS22525 0.28 +0.3 40,311 + glpE COO64_RS22525 0.58 -0.1 40,312 - glpE COO64_RS22525 0.58 -0.3 40,344 + glpE COO64_RS22525 0.68 +0.8 40,344 + glpE COO64_RS22525 0.68 -1.8 40,345 - glpE COO64_RS22525 0.68 -0.2 40,374 + glpE COO64_RS22525 0.77 -0.1 40,375 - glpE COO64_RS22525 0.78 -0.1 40,375 - glpE COO64_RS22525 0.78 +0.7 40,375 - glpE COO64_RS22525 0.78 -0.8 40,414 - glpE COO64_RS22525 0.89 -1.0 40,525 + -0.4 40,525 + +0.2 40,525 + +0.9 40,525 + +0.1 40,525 + -0.4 40,526 - +0.1 40,526 - -0.4 40,550 + +0.4 40,557 + +0.1 40,558 - -0.3 40,558 - +0.1 40,558 - -0.4 40,558 - -0.1 40,558 - -0.4 40,564 - -0.8 40,564 - +0.7 40,596 + -0.2 40,596 + -0.5 40,629 + +0.5 40,629 + -1.6 40,629 + +0.5 40,630 - +0.0 40,630 - +0.1 40,630 - -0.7 40,630 - -0.3 40,645 + +0.1 40,645 + -0.6 40,647 + -0.8 40,647 + -0.1 40,647 + -0.4 40,647 + -0.2 40,647 + +0.2 40,647 + -0.9 40,647 + -0.3 40,647 + -0.2 40,647 + -1.9 40,647 + -0.1 40,648 - -0.0 40,648 - -0.4 40,648 - +0.9 40,648 - +1.0 40,648 - -0.9 40,648 - +0.3 40,648 - -1.5 40,648 - +0.4 40,648 - -1.1 40,648 - +0.2 40,648 - -0.7 40,648 - +0.5 40,648 - +0.5 40,648 - -1.1 40,648 - -0.4 40,648 - +0.6 40,648 - -0.0 40,711 + -0.1 40,735 + +0.4 40,735 + -2.6 40,736 - +1.7 40,736 - +0.5 40,769 - +0.4 40,799 + +0.4 40,800 - -0.3 40,800 - +0.6 40,832 + -0.6 40,832 + -1.4 40,832 + +0.2 40,832 + -0.1 40,833 - -1.5 40,833 - +0.7 41,000 + COO64_RS22530 0.14 +1.2 41,000 + COO64_RS22530 0.14 -0.3 41,000 + COO64_RS22530 0.14 -0.5 41,000 + COO64_RS22530 0.14 +0.2 41,048 + COO64_RS22530 0.17 -0.7 41,049 - COO64_RS22530 0.17 +0.1 41,055 + COO64_RS22530 0.17 +0.1 41,056 - COO64_RS22530 0.17 -0.4 41,056 - COO64_RS22530 0.17 -0.2 41,056 - COO64_RS22530 0.17 +0.2 41,074 + COO64_RS22530 0.18 -0.1 41,126 + COO64_RS22530 0.21 +0.4 41,126 + COO64_RS22530 0.21 -0.8 41,126 + COO64_RS22530 0.21 +0.2 41,126 + COO64_RS22530 0.21 -1.5 41,126 + COO64_RS22530 0.21 +0.1 41,126 + COO64_RS22530 0.21 +0.6 41,126 + COO64_RS22530 0.21 -0.0 41,126 + COO64_RS22530 0.21 -0.6 41,126 + COO64_RS22530 0.21 -0.1 41,126 + COO64_RS22530 0.21 +0.1 41,126 + COO64_RS22530 0.21 +0.2 41,127 - COO64_RS22530 0.21 +0.2 41,127 - COO64_RS22530 0.21 -0.7 41,127 - COO64_RS22530 0.21 -0.2 41,127 - COO64_RS22530 0.21 +0.9 41,127 - COO64_RS22530 0.21 -0.4 41,127 - COO64_RS22530 0.21 -1.1 41,127 - COO64_RS22530 0.21 -0.1 41,127 - COO64_RS22530 0.21 -0.9 41,127 - COO64_RS22530 0.21 +0.2 41,127 - COO64_RS22530 0.21 +1.1 41,127 - COO64_RS22530 0.21 +0.1 41,127 - COO64_RS22530 0.21 -0.4 41,228 + COO64_RS22530 0.26 +0.2 41,316 - COO64_RS22530 0.30 +0.0 41,380 + COO64_RS22530 0.34 -0.1 41,380 + COO64_RS22530 0.34 +0.1 41,380 + COO64_RS22530 0.34 -0.7 41,381 - COO64_RS22530 0.34 +0.3 41,426 + COO64_RS22530 0.36 +0.9 41,426 + COO64_RS22530 0.36 -0.9 41,426 + COO64_RS22530 0.36 -1.1 41,427 - COO64_RS22530 0.36 -0.2
Or see this region's nucleotide sequence