Experiment: Supernatant; Pseudomonas DC3000 grown in LB; 0.5 X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ettA and gdhA are separated by 243 nucleotides gdhA and COO64_RS03015 overlap by 4 nucleotides COO64_RS03015 and COO64_RS03020 are separated by 66 nucleotides
COO64_RS03005: ettA - energy-dependent translational throttle protein EttA, at 81,645 to 83,309
ettA
COO64_RS03010: gdhA - NADP-specific glutamate dehydrogenase, at 83,553 to 84,890
gdhA
COO64_RS03015: COO64_RS03015 - GreA/GreB family elongation factor, at 84,887 to 85,384
_RS03015
COO64_RS03020: COO64_RS03020 - hypothetical protein, at 85,451 to 85,807
_RS03020
Position (kb) 83
84
85 Strain fitness (log2 ratio) -2
-1
0
1
2 at 82.578 kb on + strand, within ettA at 82.578 kb on + strand, within ettA at 82.578 kb on + strand, within ettA at 82.578 kb on + strand, within ettA at 82.579 kb on - strand, within ettA at 82.579 kb on - strand, within ettA at 82.641 kb on + strand, within ettA at 82.642 kb on - strand, within ettA at 82.902 kb on + strand, within ettA at 82.903 kb on - strand, within ettA at 82.903 kb on - strand, within ettA at 82.923 kb on + strand, within ettA at 82.923 kb on + strand, within ettA at 82.923 kb on + strand, within ettA at 82.923 kb on + strand, within ettA at 82.923 kb on + strand, within ettA at 82.923 kb on + strand, within ettA at 82.924 kb on - strand, within ettA at 82.924 kb on - strand, within ettA at 82.924 kb on - strand, within ettA at 83.184 kb on + strand at 83.185 kb on - strand at 83.224 kb on - strand at 83.224 kb on - strand at 83.224 kb on - strand at 83.232 kb on + strand at 83.233 kb on - strand at 83.308 kb on - strand at 83.308 kb on - strand at 83.308 kb on - strand at 83.407 kb on + strand at 83.408 kb on - strand at 83.408 kb on - strand at 83.503 kb on + strand at 83.503 kb on + strand at 83.504 kb on - strand at 83.504 kb on - strand at 83.524 kb on + strand at 83.525 kb on - strand at 83.550 kb on + strand at 83.550 kb on + strand at 83.550 kb on + strand at 83.550 kb on + strand at 83.550 kb on + strand at 83.550 kb on + strand at 83.550 kb on + strand at 83.551 kb on - strand at 83.551 kb on - strand at 83.551 kb on - strand at 83.679 kb on + strand at 83.706 kb on - strand, within gdhA at 83.706 kb on - strand, within gdhA at 83.706 kb on - strand, within gdhA at 83.706 kb on - strand, within gdhA at 83.706 kb on - strand, within gdhA at 83.706 kb on - strand, within gdhA at 83.817 kb on + strand, within gdhA at 83.817 kb on + strand, within gdhA at 83.817 kb on + strand, within gdhA at 83.818 kb on - strand, within gdhA at 83.818 kb on - strand, within gdhA at 83.818 kb on - strand, within gdhA at 84.093 kb on + strand, within gdhA at 84.093 kb on + strand, within gdhA at 84.093 kb on + strand, within gdhA at 84.094 kb on - strand, within gdhA at 84.094 kb on - strand, within gdhA at 84.094 kb on - strand, within gdhA at 84.147 kb on + strand, within gdhA at 84.147 kb on + strand, within gdhA at 84.148 kb on - strand, within gdhA at 84.195 kb on + strand, within gdhA at 84.196 kb on - strand, within gdhA at 84.196 kb on - strand, within gdhA at 84.196 kb on - strand, within gdhA at 84.282 kb on + strand, within gdhA at 84.282 kb on + strand, within gdhA at 84.283 kb on - strand, within gdhA at 84.283 kb on - strand, within gdhA at 84.351 kb on + strand, within gdhA at 84.351 kb on + strand, within gdhA at 84.351 kb on + strand, within gdhA at 84.351 kb on + strand, within gdhA at 84.352 kb on - strand, within gdhA at 84.617 kb on + strand, within gdhA at 84.617 kb on + strand, within gdhA at 84.792 kb on + strand at 84.793 kb on - strand at 84.793 kb on - strand at 84.819 kb on + strand at 84.820 kb on - strand at 84.820 kb on - strand at 84.888 kb on + strand at 84.888 kb on + strand at 84.888 kb on + strand at 84.888 kb on + strand at 84.889 kb on - strand at 84.889 kb on - strand at 84.889 kb on - strand at 85.026 kb on + strand, within COO64_RS03015 at 85.026 kb on + strand, within COO64_RS03015 at 85.027 kb on - strand, within COO64_RS03015 at 85.168 kb on - strand, within COO64_RS03015 at 85.168 kb on - strand, within COO64_RS03015 at 85.452 kb on + strand at 85.452 kb on + strand at 85.452 kb on + strand at 85.452 kb on + strand at 85.452 kb on + strand at 85.452 kb on + strand at 85.452 kb on + strand at 85.453 kb on - strand at 85.453 kb on - strand at 85.453 kb on - strand at 85.453 kb on - strand at 85.537 kb on - strand, within COO64_RS03020 at 85.605 kb on + strand, within COO64_RS03020 at 85.606 kb on - strand, within COO64_RS03020 at 85.606 kb on - strand, within COO64_RS03020 at 85.624 kb on - strand, within COO64_RS03020 at 85.624 kb on - strand, within COO64_RS03020 at 85.778 kb on + strand at 85.779 kb on - strand at 85.838 kb on + strand at 85.839 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Pseudomonas DC3000 grown in LB; 0.5 X remove 82,578 + ettA COO64_RS03005 0.56 +0.3 82,578 + ettA COO64_RS03005 0.56 -0.8 82,578 + ettA COO64_RS03005 0.56 -0.5 82,578 + ettA COO64_RS03005 0.56 -1.3 82,579 - ettA COO64_RS03005 0.56 +0.6 82,579 - ettA COO64_RS03005 0.56 -1.8 82,641 + ettA COO64_RS03005 0.60 +0.1 82,642 - ettA COO64_RS03005 0.60 +0.1 82,902 + ettA COO64_RS03005 0.75 +0.4 82,903 - ettA COO64_RS03005 0.76 -0.3 82,903 - ettA COO64_RS03005 0.76 -1.2 82,923 + ettA COO64_RS03005 0.77 -0.1 82,923 + ettA COO64_RS03005 0.77 +0.3 82,923 + ettA COO64_RS03005 0.77 +1.0 82,923 + ettA COO64_RS03005 0.77 -0.5 82,923 + ettA COO64_RS03005 0.77 +1.5 82,923 + ettA COO64_RS03005 0.77 +0.1 82,924 - ettA COO64_RS03005 0.77 -0.4 82,924 - ettA COO64_RS03005 0.77 -0.1 82,924 - ettA COO64_RS03005 0.77 -0.9 83,184 + +0.2 83,185 - +0.0 83,224 - -0.8 83,224 - -1.1 83,224 - +0.2 83,232 + -0.0 83,233 - +1.0 83,308 - -0.1 83,308 - -0.9 83,308 - -1.0 83,407 + -0.7 83,408 - +0.7 83,408 - +2.0 83,503 + +0.3 83,503 + -1.2 83,504 - -0.0 83,504 - +0.2 83,524 + +0.3 83,525 - -0.3 83,550 + -0.0 83,550 + -0.2 83,550 + -0.7 83,550 + -0.8 83,550 + +0.2 83,550 + -0.2 83,550 + -0.5 83,551 - +0.2 83,551 - +0.1 83,551 - -0.4 83,679 + -0.0 83,706 - gdhA COO64_RS03010 0.11 -0.1 83,706 - gdhA COO64_RS03010 0.11 -0.4 83,706 - gdhA COO64_RS03010 0.11 +0.5 83,706 - gdhA COO64_RS03010 0.11 -0.4 83,706 - gdhA COO64_RS03010 0.11 +1.1 83,706 - gdhA COO64_RS03010 0.11 +0.8 83,817 + gdhA COO64_RS03010 0.20 -0.5 83,817 + gdhA COO64_RS03010 0.20 +0.2 83,817 + gdhA COO64_RS03010 0.20 -0.0 83,818 - gdhA COO64_RS03010 0.20 -0.7 83,818 - gdhA COO64_RS03010 0.20 -0.4 83,818 - gdhA COO64_RS03010 0.20 -0.1 84,093 + gdhA COO64_RS03010 0.40 +0.2 84,093 + gdhA COO64_RS03010 0.40 +0.2 84,093 + gdhA COO64_RS03010 0.40 +0.1 84,094 - gdhA COO64_RS03010 0.40 -0.6 84,094 - gdhA COO64_RS03010 0.40 +0.9 84,094 - gdhA COO64_RS03010 0.40 -0.3 84,147 + gdhA COO64_RS03010 0.44 -0.6 84,147 + gdhA COO64_RS03010 0.44 -1.0 84,148 - gdhA COO64_RS03010 0.44 +0.2 84,195 + gdhA COO64_RS03010 0.48 +0.5 84,196 - gdhA COO64_RS03010 0.48 +0.4 84,196 - gdhA COO64_RS03010 0.48 -0.9 84,196 - gdhA COO64_RS03010 0.48 +0.7 84,282 + gdhA COO64_RS03010 0.54 +0.1 84,282 + gdhA COO64_RS03010 0.54 +0.4 84,283 - gdhA COO64_RS03010 0.55 +0.6 84,283 - gdhA COO64_RS03010 0.55 -0.1 84,351 + gdhA COO64_RS03010 0.60 +0.8 84,351 + gdhA COO64_RS03010 0.60 +0.7 84,351 + gdhA COO64_RS03010 0.60 -1.1 84,351 + gdhA COO64_RS03010 0.60 +0.7 84,352 - gdhA COO64_RS03010 0.60 +0.0 84,617 + gdhA COO64_RS03010 0.80 +1.5 84,617 + gdhA COO64_RS03010 0.80 -0.8 84,792 + +0.5 84,793 - -0.2 84,793 - -0.1 84,819 + -0.1 84,820 - +0.1 84,820 - -0.2 84,888 + -0.3 84,888 + -0.0 84,888 + -1.2 84,888 + -0.1 84,889 - +0.2 84,889 - +0.3 84,889 - -1.2 85,026 + COO64_RS03015 0.28 +0.1 85,026 + COO64_RS03015 0.28 -1.1 85,027 - COO64_RS03015 0.28 +1.6 85,168 - COO64_RS03015 0.56 -0.8 85,168 - COO64_RS03015 0.56 +0.3 85,452 + +1.9 85,452 + +0.9 85,452 + -0.3 85,452 + -0.9 85,452 + +0.1 85,452 + -2.2 85,452 + -2.2 85,453 - -0.4 85,453 - -0.9 85,453 - -1.0 85,453 - +1.0 85,537 - COO64_RS03020 0.24 +0.4 85,605 + COO64_RS03020 0.43 -0.1 85,606 - COO64_RS03020 0.43 +0.1 85,606 - COO64_RS03020 0.43 -0.6 85,624 - COO64_RS03020 0.48 +1.5 85,624 - COO64_RS03020 0.48 +0.5 85,778 + -1.5 85,779 - -0.2 85,838 + +0.8 85,839 - +0.5
Or see this region's nucleotide sequence