Experiment: Ying_Nucleobase14 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5665 and GFF5666 are separated by 3 nucleotides GFF5666 and GFF5667 overlap by 4 nucleotides GFF5667 and GFF5668 overlap by 4 nucleotides
GFF5665 - Lipid carrier protein or keto acyl-CoA thiolase Ltp3 (EC 2.3.1.16), at 2,688,816 to 2,690,018
GFF5665
GFF5666 - Hydroxymethylglutaryl-CoA synthase (EC 2.3.3.10), at 2,690,022 to 2,691,500
GFF5666
GFF5667 - 6-hexanolactone hydrolase, at 2,691,497 to 2,692,399
GFF5667
GFF5668 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 2,692,396 to 2,693,145
GFF5668
Position (kb)
2690
2691
2692 Strain fitness (log2 ratio)
-1
0
1 at 2689.127 kb on - strand, within GFF5665 at 2689.192 kb on + strand, within GFF5665 at 2689.228 kb on + strand, within GFF5665 at 2689.228 kb on + strand, within GFF5665 at 2689.229 kb on - strand, within GFF5665 at 2689.387 kb on - strand, within GFF5665 at 2689.388 kb on - strand, within GFF5665 at 2689.604 kb on - strand, within GFF5665 at 2689.619 kb on - strand, within GFF5665 at 2689.720 kb on + strand, within GFF5665 at 2690.023 kb on + strand at 2690.342 kb on - strand, within GFF5666 at 2690.518 kb on + strand, within GFF5666 at 2690.519 kb on - strand, within GFF5666 at 2691.470 kb on - strand at 2691.470 kb on - strand at 2692.188 kb on + strand, within GFF5667
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2 remove 2,689,127 - GFF5665 0.26 -0.1 2,689,192 + GFF5665 0.31 -0.1 2,689,228 + GFF5665 0.34 +0.3 2,689,228 + GFF5665 0.34 -0.3 2,689,229 - GFF5665 0.34 -0.5 2,689,387 - GFF5665 0.47 -0.3 2,689,388 - GFF5665 0.48 -0.1 2,689,604 - GFF5665 0.66 +0.6 2,689,619 - GFF5665 0.67 +0.1 2,689,720 + GFF5665 0.75 +0.3 2,690,023 + +0.3 2,690,342 - GFF5666 0.22 -0.8 2,690,518 + GFF5666 0.34 -1.0 2,690,519 - GFF5666 0.34 +0.9 2,691,470 - +0.8 2,691,470 - -0.6 2,692,188 + GFF5667 0.77 -0.8
Or see this region's nucleotide sequence