Experiment: Ying_Nucleobase14 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5636 and GFF5637 overlap by 4 nucleotides GFF5637 and GFF5638 overlap by 4 nucleotides GFF5638 and GFF5639 are separated by 10 nucleotides
GFF5636 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 2,658,124 to 2,658,897
GFF5636
GFF5637 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 2,658,894 to 2,659,706
GFF5637
GFF5638 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 2,659,703 to 2,660,788
GFF5638
GFF5639 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 2,660,799 to 2,661,674
GFF5639
Position (kb)
2659
2660
2661 Strain fitness (log2 ratio)
-1
0
1 at 2658.953 kb on - strand at 2660.071 kb on - strand, within GFF5638 at 2660.419 kb on - strand, within GFF5638 at 2660.631 kb on + strand, within GFF5638 at 2660.763 kb on + strand at 2661.133 kb on + strand, within GFF5639 at 2661.299 kb on - strand, within GFF5639 at 2661.593 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2 remove 2,658,953 - +0.6 2,660,071 - GFF5638 0.34 -1.3 2,660,419 - GFF5638 0.66 -1.1 2,660,631 + GFF5638 0.85 +0.0 2,660,763 + -0.1 2,661,133 + GFF5639 0.38 -0.2 2,661,299 - GFF5639 0.57 -0.4 2,661,593 - -0.8
Or see this region's nucleotide sequence