Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF5638

Experiment: Ying_Nucleobase14 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5636 and GFF5637 overlap by 4 nucleotidesGFF5637 and GFF5638 overlap by 4 nucleotidesGFF5638 and GFF5639 are separated by 10 nucleotides GFF5636 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 2,658,124 to 2,658,897 GFF5636 GFF5637 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 2,658,894 to 2,659,706 GFF5637 GFF5638 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 2,659,703 to 2,660,788 GFF5638 GFF5639 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 2,660,799 to 2,661,674 GFF5639 Position (kb) 2659 2660 2661Strain fitness (log2 ratio) -1 0 1at 2658.953 kb on - strandat 2660.071 kb on - strand, within GFF5638at 2660.419 kb on - strand, within GFF5638at 2660.631 kb on + strand, within GFF5638at 2660.763 kb on + strandat 2661.133 kb on + strand, within GFF5639at 2661.299 kb on - strand, within GFF5639at 2661.593 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2
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2,658,953 - +0.6
2,660,071 - GFF5638 0.34 -1.3
2,660,419 - GFF5638 0.66 -1.1
2,660,631 + GFF5638 0.85 +0.0
2,660,763 + -0.1
2,661,133 + GFF5639 0.38 -0.2
2,661,299 - GFF5639 0.57 -0.4
2,661,593 - -0.8

Or see this region's nucleotide sequence