Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF5448

Experiment: Ying_Nucleobase14 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5446 and GFF5447 are separated by 51 nucleotidesGFF5447 and GFF5448 are separated by 7 nucleotidesGFF5448 and GFF5449 are separated by 74 nucleotides GFF5446 - carbonic anhydrase, family 3, at 2,448,887 to 2,449,411 GFF5446 GFF5447 - COG2833: uncharacterized protein, at 2,449,463 to 2,450,296 GFF5447 GFF5448 - NADH-ubiquinone oxidoreductase, putative, at 2,450,304 to 2,451,284 GFF5448 GFF5449 - ATP-dependent 23S rRNA helicase DbpA, at 2,451,359 to 2,452,753 GFF5449 Position (kb) 2450 2451 2452Strain fitness (log2 ratio) -1 0 1at 2449.438 kb on + strandat 2449.446 kb on + strandat 2449.447 kb on - strandat 2449.814 kb on + strand, within GFF5447at 2449.814 kb on + strand, within GFF5447at 2449.815 kb on - strand, within GFF5447at 2449.899 kb on - strand, within GFF5447at 2450.135 kb on + strand, within GFF5447at 2450.434 kb on + strand, within GFF5448at 2450.566 kb on + strand, within GFF5448at 2450.567 kb on - strand, within GFF5448at 2451.333 kb on + strandat 2451.333 kb on + strandat 2451.351 kb on - strandat 2451.437 kb on + strandat 2451.437 kb on + strandat 2452.031 kb on + strand, within GFF5449at 2452.031 kb on + strand, within GFF5449

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2
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2,449,438 + -0.4
2,449,446 + +0.5
2,449,447 - -1.1
2,449,814 + GFF5447 0.42 -0.3
2,449,814 + GFF5447 0.42 +0.4
2,449,815 - GFF5447 0.42 +0.1
2,449,899 - GFF5447 0.52 -0.8
2,450,135 + GFF5447 0.81 +0.1
2,450,434 + GFF5448 0.13 +0.1
2,450,566 + GFF5448 0.27 -0.4
2,450,567 - GFF5448 0.27 -0.8
2,451,333 + +0.5
2,451,333 + -0.2
2,451,351 - +0.5
2,451,437 + +0.2
2,451,437 + -1.1
2,452,031 + GFF5449 0.48 -1.2
2,452,031 + GFF5449 0.48 -0.4

Or see this region's nucleotide sequence