Experiment: Ying_Nucleobase14 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5210 and GFF5211 are separated by 120 nucleotides GFF5211 and GFF5212 are separated by 235 nucleotides GFF5212 and GFF5213 overlap by 42 nucleotides GFF5213 and GFF5214 are separated by 36 nucleotides GFF5214 and GFF5215 are separated by 29 nucleotides
GFF5210 - hypothetical protein, at 2,225,174 to 2,226,823
GFF5210
GFF5211 - hypothetical protein, at 2,226,944 to 2,227,438
GFF5211
GFF5212 - hypothetical protein, at 2,227,674 to 2,227,802
GFF5212
GFF5213 - Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10), at 2,227,761 to 2,228,891
GFF5213
GFF5214 - Glycine cleavage system H protein, at 2,228,928 to 2,229,296
GFF5214
GFF5215 - Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2), at 2,229,326 to 2,232,235
GFF5215
Position (kb)
2227
2228
2229 Strain fitness (log2 ratio)
-1
0
1 at 2226.821 kb on + strand at 2226.821 kb on + strand at 2226.821 kb on + strand at 2226.822 kb on - strand at 2226.822 kb on - strand at 2226.822 kb on - strand at 2227.119 kb on - strand, within GFF5211 at 2227.467 kb on - strand at 2227.600 kb on - strand at 2227.679 kb on + strand at 2227.680 kb on - strand at 2227.849 kb on - strand at 2228.080 kb on + strand, within GFF5213 at 2228.478 kb on + strand, within GFF5213 at 2228.877 kb on + strand at 2228.941 kb on - strand at 2229.120 kb on + strand, within GFF5214 at 2229.120 kb on + strand, within GFF5214 at 2229.120 kb on + strand, within GFF5214 at 2229.120 kb on + strand, within GFF5214 at 2229.121 kb on - strand, within GFF5214 at 2229.274 kb on - strand at 2229.662 kb on + strand, within GFF5215 at 2229.663 kb on - strand, within GFF5215 at 2229.663 kb on - strand, within GFF5215 at 2229.671 kb on + strand, within GFF5215 at 2229.672 kb on - strand, within GFF5215 at 2229.672 kb on - strand, within GFF5215 at 2229.672 kb on - strand, within GFF5215 at 2229.680 kb on + strand, within GFF5215
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2 remove 2,226,821 + +0.0 2,226,821 + +0.5 2,226,821 + +1.0 2,226,822 - +0.1 2,226,822 - +1.1 2,226,822 - -0.1 2,227,119 - GFF5211 0.35 -0.3 2,227,467 - -0.1 2,227,600 - -0.1 2,227,679 + -0.2 2,227,680 - +0.1 2,227,849 - -1.4 2,228,080 + GFF5213 0.28 -0.4 2,228,478 + GFF5213 0.63 -0.3 2,228,877 + -0.9 2,228,941 - +0.1 2,229,120 + GFF5214 0.52 +0.7 2,229,120 + GFF5214 0.52 +0.5 2,229,120 + GFF5214 0.52 +1.3 2,229,120 + GFF5214 0.52 +0.6 2,229,121 - GFF5214 0.52 -0.3 2,229,274 - +0.3 2,229,662 + GFF5215 0.12 -0.7 2,229,663 - GFF5215 0.12 -0.0 2,229,663 - GFF5215 0.12 +0.4 2,229,671 + GFF5215 0.12 -0.0 2,229,672 - GFF5215 0.12 -0.3 2,229,672 - GFF5215 0.12 -0.9 2,229,672 - GFF5215 0.12 +0.2 2,229,680 + GFF5215 0.12 +0.1
Or see this region's nucleotide sequence