Experiment: Ying_Nucleobase14 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4174 and GFF4175 overlap by 4 nucleotides GFF4175 and GFF4176 overlap by 4 nucleotides GFF4176 and GFF4177 overlap by 4 nucleotides GFF4177 and GFF4178 overlap by 4 nucleotides GFF4178 and GFF4179 are separated by 15 nucleotides
GFF4174 - Type IV secretion system protein VirD4, at 1,090,443 to 1,092,443
GFF4174
GFF4175 - CopG domain-containing protein, at 1,092,440 to 1,092,904
GFF4175
GFF4176 - Conjugative transfer protein TrbB, at 1,092,901 to 1,093,953
GFF4176
GFF4177 - Conjugative transfer protein TrbC, at 1,093,950 to 1,094,333
GFF4177
GFF4178 - Conjugative transfer protein TrbD, at 1,094,330 to 1,094,602
GFF4178
GFF4179 - Conjugative transfer protein TrbE, at 1,094,618 to 1,097,071
GFF4179
Position (kb)
1092
1093
1094 Strain fitness (log2 ratio)
-1
0
1 at 1091.913 kb on + strand, within GFF4174 at 1091.914 kb on - strand, within GFF4174 at 1091.914 kb on - strand, within GFF4174 at 1091.914 kb on - strand, within GFF4174 at 1091.942 kb on - strand, within GFF4174 at 1092.076 kb on - strand, within GFF4174 at 1092.439 kb on + strand at 1092.439 kb on + strand at 1092.439 kb on + strand at 1092.449 kb on + strand at 1092.637 kb on + strand, within GFF4175 at 1092.638 kb on - strand, within GFF4175 at 1092.689 kb on + strand, within GFF4175 at 1092.689 kb on + strand, within GFF4175 at 1092.692 kb on + strand, within GFF4175 at 1092.692 kb on + strand, within GFF4175 at 1092.692 kb on + strand, within GFF4175 at 1092.692 kb on + strand, within GFF4175 at 1092.692 kb on + strand, within GFF4175 at 1092.693 kb on - strand, within GFF4175 at 1092.693 kb on - strand, within GFF4175 at 1093.289 kb on - strand, within GFF4176 at 1093.760 kb on + strand, within GFF4176 at 1093.761 kb on - strand, within GFF4176 at 1093.761 kb on - strand, within GFF4176 at 1094.328 kb on + strand at 1094.329 kb on + strand at 1094.329 kb on + strand at 1094.842 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2 remove 1,091,913 + GFF4174 0.73 +0.8 1,091,914 - GFF4174 0.74 +1.2 1,091,914 - GFF4174 0.74 -1.0 1,091,914 - GFF4174 0.74 -0.9 1,091,942 - GFF4174 0.75 -0.1 1,092,076 - GFF4174 0.82 +0.0 1,092,439 + -1.0 1,092,439 + +0.5 1,092,439 + -0.2 1,092,449 + +0.1 1,092,637 + GFF4175 0.42 +0.1 1,092,638 - GFF4175 0.43 -0.2 1,092,689 + GFF4175 0.54 -0.1 1,092,689 + GFF4175 0.54 -0.2 1,092,692 + GFF4175 0.54 +1.0 1,092,692 + GFF4175 0.54 -0.1 1,092,692 + GFF4175 0.54 +0.6 1,092,692 + GFF4175 0.54 +0.3 1,092,692 + GFF4175 0.54 +0.5 1,092,693 - GFF4175 0.54 -0.1 1,092,693 - GFF4175 0.54 +0.3 1,093,289 - GFF4176 0.37 -0.0 1,093,760 + GFF4176 0.82 +0.2 1,093,761 - GFF4176 0.82 +0.4 1,093,761 - GFF4176 0.82 -0.7 1,094,328 + +0.0 1,094,329 + +0.3 1,094,329 + -0.1 1,094,842 - -0.4
Or see this region's nucleotide sequence