Experiment: Ying_Nucleobase14 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3235 and GFF3236 are separated by 53 nucleotides GFF3236 and GFF3237 are separated by 11 nucleotides GFF3237 and GFF3238 are separated by 119 nucleotides GFF3238 and GFF3239 are separated by 41 nucleotides
GFF3235 - FIG00576391: hypothetical protein, at 117,616 to 118,326
GFF3235
GFF3236 - UBA/THIF-type NAD/FAD binding fold, at 118,380 to 119,219
GFF3236
GFF3237 - hypothetical protein, at 119,231 to 119,434
GFF3237
GFF3238 - HNS-like transcription regulator protein, at 119,554 to 119,913
GFF3238
GFF3239 - Cys-tRNA(Pro) deacylase YbaK, at 119,955 to 120,941
GFF3239
Position (kb) 119
120 Strain fitness (log2 ratio) -1
0
1 at 118.581 kb on + strand, within GFF3236 at 119.020 kb on + strand, within GFF3236 at 119.336 kb on + strand, within GFF3237 at 119.359 kb on - strand, within GFF3237 at 119.396 kb on + strand, within GFF3237 at 119.473 kb on + strand at 119.474 kb on - strand at 119.475 kb on + strand at 119.499 kb on + strand at 119.499 kb on + strand at 119.548 kb on - strand at 119.663 kb on + strand, within GFF3238 at 119.683 kb on + strand, within GFF3238 at 119.684 kb on - strand, within GFF3238 at 120.005 kb on - strand at 120.005 kb on - strand at 120.133 kb on + strand, within GFF3239 at 120.133 kb on + strand, within GFF3239 at 120.217 kb on + strand, within GFF3239 at 120.218 kb on + strand, within GFF3239 at 120.379 kb on + strand, within GFF3239 at 120.379 kb on + strand, within GFF3239 at 120.380 kb on - strand, within GFF3239
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2 remove 118,581 + GFF3236 0.24 -1.0 119,020 + GFF3236 0.76 -0.1 119,336 + GFF3237 0.51 +0.3 119,359 - GFF3237 0.63 +0.2 119,396 + GFF3237 0.81 +0.3 119,473 + -0.2 119,474 - +1.3 119,475 + -0.5 119,499 + +0.5 119,499 + +0.3 119,548 - +1.0 119,663 + GFF3238 0.30 -0.4 119,683 + GFF3238 0.36 -0.3 119,684 - GFF3238 0.36 -0.4 120,005 - +0.1 120,005 - -0.2 120,133 + GFF3239 0.18 +0.1 120,133 + GFF3239 0.18 -0.4 120,217 + GFF3239 0.27 -0.5 120,218 + GFF3239 0.27 -0.3 120,379 + GFF3239 0.43 -0.0 120,379 + GFF3239 0.43 +0.7 120,380 - GFF3239 0.43 -1.0
Or see this region's nucleotide sequence