Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF28

Experiment: Ying_Nucleobase14 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF26 and GFF27 are separated by 104 nucleotidesGFF27 and GFF28 are separated by 12 nucleotidesGFF28 and GFF29 are separated by 174 nucleotidesGFF29 and GFF30 are separated by 15 nucleotides GFF26 - hypothetical protein, at 24,311 to 25,072 GFF26 GFF27 - Pca regulon regulatory protein PcaR, at 25,177 to 25,941 GFF27 GFF28 - Mannose-6-phosphate isomerase (EC 5.3.1.8), at 25,954 to 26,481 GFF28 GFF29 - Transcriptional regulator, MarR family, at 26,656 to 27,123 GFF29 GFF30 - Cytosine deaminase (EC 3.5.4.1), at 27,139 to 28,488 GFF30 Position (kb) 25 26 27Strain fitness (log2 ratio) -2 -1 0 1 2at 25.057 kb on + strandat 25.057 kb on + strandat 25.057 kb on + strandat 25.057 kb on + strandat 25.058 kb on - strandat 25.294 kb on + strand, within GFF27at 25.295 kb on - strand, within GFF27at 25.321 kb on + strand, within GFF27at 25.322 kb on - strand, within GFF27at 25.385 kb on - strand, within GFF27at 25.390 kb on + strand, within GFF27at 25.390 kb on + strand, within GFF27at 25.391 kb on - strand, within GFF27at 25.391 kb on - strand, within GFF27at 25.501 kb on + strand, within GFF27at 25.501 kb on + strand, within GFF27at 25.501 kb on + strand, within GFF27at 25.501 kb on + strand, within GFF27at 25.502 kb on - strand, within GFF27at 25.502 kb on - strand, within GFF27at 25.570 kb on + strand, within GFF27at 25.570 kb on + strand, within GFF27at 25.570 kb on + strand, within GFF27at 25.571 kb on - strand, within GFF27at 25.726 kb on + strand, within GFF27at 25.726 kb on + strand, within GFF27at 25.727 kb on - strand, within GFF27at 25.727 kb on - strand, within GFF27at 25.852 kb on + strand, within GFF27at 25.853 kb on - strand, within GFF27at 26.313 kb on - strand, within GFF28at 26.313 kb on - strand, within GFF28at 26.313 kb on - strand, within GFF28at 26.378 kb on + strand, within GFF28at 26.532 kb on - strandat 26.547 kb on + strandat 26.555 kb on - strandat 26.657 kb on + strandat 26.658 kb on - strandat 26.658 kb on - strandat 26.658 kb on - strandat 26.963 kb on + strand, within GFF29at 26.963 kb on + strand, within GFF29at 26.964 kb on - strand, within GFF29at 26.964 kb on - strand, within GFF29at 27.236 kb on + strandat 27.237 kb on - strandat 27.237 kb on - strandat 27.413 kb on + strand, within GFF30at 27.414 kb on - strand, within GFF30at 27.428 kb on + strand, within GFF30at 27.429 kb on - strand, within GFF30

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2
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25,057 + -0.3
25,057 + -0.6
25,057 + -0.1
25,057 + +0.5
25,058 - +0.0
25,294 + GFF27 0.15 +0.7
25,295 - GFF27 0.15 -2.0
25,321 + GFF27 0.19 +0.3
25,322 - GFF27 0.19 -0.5
25,385 - GFF27 0.27 -0.4
25,390 + GFF27 0.28 -0.2
25,390 + GFF27 0.28 +0.4
25,391 - GFF27 0.28 +0.0
25,391 - GFF27 0.28 +0.5
25,501 + GFF27 0.42 +0.8
25,501 + GFF27 0.42 -0.3
25,501 + GFF27 0.42 -0.4
25,501 + GFF27 0.42 -0.6
25,502 - GFF27 0.42 -0.5
25,502 - GFF27 0.42 -0.0
25,570 + GFF27 0.51 -2.1
25,570 + GFF27 0.51 +0.5
25,570 + GFF27 0.51 -0.1
25,571 - GFF27 0.52 +0.8
25,726 + GFF27 0.72 -0.2
25,726 + GFF27 0.72 +0.4
25,727 - GFF27 0.72 +0.4
25,727 - GFF27 0.72 +1.0
25,852 + GFF27 0.88 -0.4
25,853 - GFF27 0.88 +1.0
26,313 - GFF28 0.68 -0.5
26,313 - GFF28 0.68 -0.1
26,313 - GFF28 0.68 -1.6
26,378 + GFF28 0.80 +0.1
26,532 - -0.3
26,547 + +1.8
26,555 - -0.7
26,657 + -0.5
26,658 - -0.7
26,658 - -0.1
26,658 - +0.1
26,963 + GFF29 0.66 +1.0
26,963 + GFF29 0.66 -1.5
26,964 - GFF29 0.66 -0.3
26,964 - GFF29 0.66 -1.4
27,236 + +0.3
27,237 - -0.1
27,237 - +0.1
27,413 + GFF30 0.20 +0.9
27,414 - GFF30 0.20 +0.8
27,428 + GFF30 0.21 -0.2
27,429 - GFF30 0.21 -0.9

Or see this region's nucleotide sequence