Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF2096

Experiment: Ying_Nucleobase14 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2094 and GFF2095 overlap by 4 nucleotidesGFF2095 and GFF2096 are separated by 251 nucleotidesGFF2096 and GFF2097 overlap by 4 nucleotidesGFF2097 and GFF2098 overlap by 4 nucleotides GFF2094 - TRAP-type C4-dicarboxylate transport system, large permease component, at 981,212 to 982,489 GFF2094 GFF2095 - TRAP-type C4-dicarboxylate transport system, small permease component, at 982,486 to 983,016 GFF2095 GFF2096 - D-2-hydroxyglutarate dehydrogenase, at 983,268 to 984,656 GFF2096 GFF2097 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 984,653 to 985,621 GFF2097 GFF2098 - Dihydroxy-acid dehydratase (EC 4.2.1.9), at 985,618 to 987,507 GFF2098 Position (kb) 983 984 985Strain fitness (log2 ratio) -1 0 1at 983.101 kb on + strandat 983.101 kb on + strandat 983.102 kb on - strandat 983.736 kb on + strand, within GFF2096at 984.043 kb on - strand, within GFF2096

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2
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983,101 + -0.1
983,101 + -0.6
983,102 - +0.2
983,736 + GFF2096 0.34 -0.6
984,043 - GFF2096 0.56 -0.1

Or see this region's nucleotide sequence