Experiment: Ying_Nucleobase14 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF187 and GFF188 are separated by 13 nucleotides GFF188 and GFF189 are separated by 48 nucleotides GFF189 and GFF190 are separated by 24 nucleotides
GFF187 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 199,662 to 200,624
GFF187
GFF188 - probable extracellular solute-binding protein, at 200,638 to 201,387
GFF188
GFF189 - MFS permease, at 201,436 to 202,833
GFF189
GFF190 - Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1), at 202,858 to 204,537
GFF190
Position (kb) 201
202
203 Strain fitness (log2 ratio) -1
0
1 at 200.987 kb on + strand, within GFF188 at 200.987 kb on + strand, within GFF188 at 200.988 kb on - strand, within GFF188 at 200.988 kb on - strand, within GFF188 at 201.074 kb on + strand, within GFF188 at 201.074 kb on + strand, within GFF188 at 201.075 kb on - strand, within GFF188 at 201.095 kb on + strand, within GFF188 at 201.095 kb on + strand, within GFF188 at 201.533 kb on + strand at 201.696 kb on - strand, within GFF189 at 201.810 kb on - strand, within GFF189 at 201.810 kb on - strand, within GFF189 at 202.685 kb on + strand, within GFF189 at 202.685 kb on + strand, within GFF189 at 202.686 kb on - strand, within GFF189 at 202.961 kb on + strand at 202.962 kb on - strand at 203.643 kb on - strand, within GFF190
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2 remove 200,987 + GFF188 0.47 -0.3 200,987 + GFF188 0.47 -1.7 200,988 - GFF188 0.47 -0.4 200,988 - GFF188 0.47 -0.0 201,074 + GFF188 0.58 +0.4 201,074 + GFF188 0.58 -1.2 201,075 - GFF188 0.58 -1.3 201,095 + GFF188 0.61 +0.3 201,095 + GFF188 0.61 +0.7 201,533 + -0.4 201,696 - GFF189 0.19 +0.4 201,810 - GFF189 0.27 +0.1 201,810 - GFF189 0.27 +0.2 202,685 + GFF189 0.89 +0.3 202,685 + GFF189 0.89 +1.1 202,686 - GFF189 0.89 +0.4 202,961 + +1.4 202,962 - +0.1 203,643 - GFF190 0.47 -0.6
Or see this region's nucleotide sequence