Experiment: Ying_Nucleobase14 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1814 and GFF1815 are separated by 5 nucleotides GFF1815 and GFF1816 are separated by 86 nucleotides GFF1816 and GFF1817 are separated by 29 nucleotides GFF1817 and GFF1818 are separated by 87 nucleotides
GFF1814 - Glutathione S-transferase (EC 2.5.1.18), at 681,900 to 682,550
GFF1814
GFF1815 - amidohydrolase 2, at 682,556 to 683,449
GFF1815
GFF1816 - Dienelactone hydrolase family, at 683,536 to 684,219
GFF1816
GFF1817 - 2-hydroxychromene-2-carboxylate isomerase, at 684,249 to 684,848
GFF1817
GFF1818 - Short chain dehydrogenase, at 684,936 to 685,694
GFF1818
Position (kb)
683
684
685 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 682.674 kb on + strand, within GFF1815 at 682.674 kb on + strand, within GFF1815 at 682.674 kb on + strand, within GFF1815 at 682.675 kb on - strand, within GFF1815 at 682.675 kb on - strand, within GFF1815 at 682.731 kb on + strand, within GFF1815 at 682.731 kb on + strand, within GFF1815 at 682.731 kb on + strand, within GFF1815 at 682.732 kb on - strand, within GFF1815 at 683.037 kb on + strand, within GFF1815 at 683.037 kb on + strand, within GFF1815 at 683.038 kb on - strand, within GFF1815 at 683.038 kb on - strand, within GFF1815 at 683.143 kb on - strand, within GFF1815 at 683.143 kb on - strand, within GFF1815 at 683.143 kb on - strand, within GFF1815 at 683.143 kb on - strand, within GFF1815 at 683.143 kb on - strand, within GFF1815 at 683.143 kb on - strand, within GFF1815 at 683.143 kb on - strand, within GFF1815 at 683.143 kb on - strand, within GFF1815 at 683.160 kb on + strand, within GFF1815 at 683.161 kb on - strand, within GFF1815 at 683.161 kb on - strand, within GFF1815 at 683.361 kb on + strand at 683.361 kb on + strand at 683.362 kb on - strand at 683.362 kb on - strand at 683.394 kb on + strand at 683.394 kb on + strand at 683.394 kb on + strand at 683.395 kb on - strand at 683.603 kb on + strand at 683.603 kb on + strand at 683.603 kb on + strand at 683.603 kb on + strand at 683.604 kb on - strand at 683.604 kb on - strand at 683.792 kb on + strand, within GFF1816 at 683.792 kb on + strand, within GFF1816 at 683.792 kb on + strand, within GFF1816 at 683.793 kb on - strand, within GFF1816 at 683.793 kb on - strand, within GFF1816 at 683.793 kb on - strand, within GFF1816 at 683.793 kb on - strand, within GFF1816 at 683.795 kb on + strand, within GFF1816 at 683.796 kb on - strand, within GFF1816 at 684.000 kb on - strand, within GFF1816 at 684.056 kb on + strand, within GFF1816 at 684.167 kb on + strand at 684.167 kb on + strand at 684.168 kb on - strand at 684.319 kb on + strand, within GFF1817 at 684.319 kb on + strand, within GFF1817 at 684.319 kb on + strand, within GFF1817 at 684.319 kb on + strand, within GFF1817 at 684.320 kb on - strand, within GFF1817 at 684.320 kb on - strand, within GFF1817 at 684.320 kb on - strand, within GFF1817 at 684.374 kb on - strand, within GFF1817 at 684.800 kb on - strand at 684.826 kb on + strand at 684.826 kb on + strand at 684.826 kb on + strand at 684.826 kb on + strand at 684.826 kb on + strand at 684.826 kb on + strand at 684.827 kb on - strand at 684.827 kb on - strand at 684.827 kb on - strand at 684.830 kb on - strand at 684.870 kb on + strand at 684.870 kb on + strand at 684.870 kb on + strand at 684.870 kb on + strand at 684.871 kb on - strand at 684.901 kb on + strand at 684.901 kb on + strand at 684.901 kb on + strand at 684.902 kb on - strand at 684.935 kb on + strand at 684.936 kb on - strand at 685.044 kb on + strand, within GFF1818 at 685.044 kb on + strand, within GFF1818 at 685.044 kb on + strand, within GFF1818 at 685.044 kb on + strand, within GFF1818 at 685.045 kb on - strand, within GFF1818 at 685.045 kb on - strand, within GFF1818 at 685.045 kb on - strand, within GFF1818
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2 remove 682,674 + GFF1815 0.13 -1.2 682,674 + GFF1815 0.13 +0.8 682,674 + GFF1815 0.13 -0.5 682,675 - GFF1815 0.13 -0.2 682,675 - GFF1815 0.13 -1.4 682,731 + GFF1815 0.20 -0.3 682,731 + GFF1815 0.20 +0.1 682,731 + GFF1815 0.20 -0.0 682,732 - GFF1815 0.20 -0.6 683,037 + GFF1815 0.54 -1.4 683,037 + GFF1815 0.54 -0.2 683,038 - GFF1815 0.54 -0.0 683,038 - GFF1815 0.54 -0.0 683,143 - GFF1815 0.66 +0.5 683,143 - GFF1815 0.66 -0.6 683,143 - GFF1815 0.66 +0.1 683,143 - GFF1815 0.66 +0.5 683,143 - GFF1815 0.66 -0.0 683,143 - GFF1815 0.66 +0.5 683,143 - GFF1815 0.66 -1.5 683,143 - GFF1815 0.66 -2.5 683,160 + GFF1815 0.68 +0.1 683,161 - GFF1815 0.68 -0.4 683,161 - GFF1815 0.68 +0.0 683,361 + +0.1 683,361 + -0.5 683,362 - -0.3 683,362 - -0.5 683,394 + +0.3 683,394 + +1.1 683,394 + -0.5 683,395 - -0.8 683,603 + +0.3 683,603 + -1.5 683,603 + -0.3 683,603 + +0.1 683,604 - -0.1 683,604 - -1.0 683,792 + GFF1816 0.37 -0.4 683,792 + GFF1816 0.37 -0.5 683,792 + GFF1816 0.37 -0.2 683,793 - GFF1816 0.38 +0.4 683,793 - GFF1816 0.38 +0.4 683,793 - GFF1816 0.38 -0.1 683,793 - GFF1816 0.38 -0.2 683,795 + GFF1816 0.38 +2.1 683,796 - GFF1816 0.38 +0.2 684,000 - GFF1816 0.68 +0.5 684,056 + GFF1816 0.76 +0.6 684,167 + -0.3 684,167 + -1.0 684,168 - -0.5 684,319 + GFF1817 0.12 +0.2 684,319 + GFF1817 0.12 +0.2 684,319 + GFF1817 0.12 +0.0 684,319 + GFF1817 0.12 +0.7 684,320 - GFF1817 0.12 -1.9 684,320 - GFF1817 0.12 +0.5 684,320 - GFF1817 0.12 +0.4 684,374 - GFF1817 0.21 +0.9 684,800 - +0.0 684,826 + -0.3 684,826 + -0.5 684,826 + -0.6 684,826 + -1.5 684,826 + +0.4 684,826 + +0.4 684,827 - +0.1 684,827 - -1.0 684,827 - +0.1 684,830 - -0.6 684,870 + -1.8 684,870 + +0.4 684,870 + -0.3 684,870 + -0.6 684,871 - -0.2 684,901 + +0.2 684,901 + +0.1 684,901 + -0.3 684,902 - +0.4 684,935 + -0.6 684,936 - +0.5 685,044 + GFF1818 0.14 -0.1 685,044 + GFF1818 0.14 +0.5 685,044 + GFF1818 0.14 -0.0 685,044 + GFF1818 0.14 -0.0 685,045 - GFF1818 0.14 +0.3 685,045 - GFF1818 0.14 +0.4 685,045 - GFF1818 0.14 -0.3
Or see this region's nucleotide sequence