Experiment: Ying_Nucleobase14 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF162 and GFF163 are separated by 13 nucleotides GFF163 and GFF164 are separated by 10 nucleotides GFF164 and GFF165 are separated by 124 nucleotides GFF165 and GFF166 are separated by 10 nucleotides
GFF162 - hypothetical protein, at 176,483 to 177,607
GFF162
GFF163 - hypothetical protein, at 177,621 to 177,890
GFF163
GFF164 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.180), at 177,901 to 178,947
GFF164
GFF165 - FIG00932121: hypothetical protein, at 179,072 to 179,527
GFF165
GFF166 - Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17), at 179,538 to 181,406
GFF166
Position (kb) 177
178
179 Strain fitness (log2 ratio) -2
-1
0
1 at 177.205 kb on - strand, within GFF162 at 177.429 kb on + strand, within GFF162 at 177.429 kb on + strand, within GFF162 at 177.429 kb on + strand, within GFF162 at 177.430 kb on - strand, within GFF162 at 177.998 kb on + strand at 177.998 kb on + strand at 178.292 kb on + strand, within GFF164 at 178.565 kb on + strand, within GFF164 at 178.566 kb on - strand, within GFF164 at 179.021 kb on - strand at 179.021 kb on - strand at 179.121 kb on + strand, within GFF165 at 179.121 kb on + strand, within GFF165 at 179.122 kb on - strand, within GFF165 at 179.122 kb on - strand, within GFF165 at 179.455 kb on - strand, within GFF165 at 179.470 kb on - strand, within GFF165 at 179.578 kb on + strand at 179.578 kb on + strand at 179.578 kb on + strand at 179.579 kb on - strand at 179.639 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2 remove 177,205 - GFF162 0.64 -0.2 177,429 + GFF162 0.84 -0.3 177,429 + GFF162 0.84 -0.1 177,429 + GFF162 0.84 +0.3 177,430 - GFF162 0.84 -0.5 177,998 + +0.9 177,998 + -1.7 178,292 + GFF164 0.37 -0.8 178,565 + GFF164 0.63 -0.6 178,566 - GFF164 0.64 +0.8 179,021 - +0.2 179,021 - +0.2 179,121 + GFF165 0.11 -0.4 179,121 + GFF165 0.11 +0.6 179,122 - GFF165 0.11 +0.5 179,122 - GFF165 0.11 +0.8 179,455 - GFF165 0.84 -0.2 179,470 - GFF165 0.87 -0.7 179,578 + -1.8 179,578 + -2.2 179,578 + -2.4 179,579 - -1.7 179,639 - -2.0
Or see this region's nucleotide sequence