Experiment: Ying_Nucleobase14 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1297 and GFF1298 are separated by 8 nucleotides GFF1298 and GFF1299 overlap by 49 nucleotides GFF1299 and GFF1300 are separated by 78 nucleotides
GFF1297 - 4-hydroxythreonine-4-phosphate dehydrogenase (EC 1.1.1.262), at 153,717 to 154,757
GFF1297
GFF1298 - FIG137478: Hypothetical protein YbgI, at 154,766 to 155,551
GFF1298
GFF1299 - hypothetical protein, at 155,503 to 155,640
GFF1299
GFF1300 - Outer membrane stress sensor protease DegS, at 155,719 to 156,867
GFF1300
Position (kb) 154
155
156 Strain fitness (log2 ratio) -2
-1
0
1 at 154.882 kb on - strand, within GFF1298 at 154.882 kb on - strand, within GFF1298 at 154.882 kb on - strand, within GFF1298 at 154.882 kb on - strand, within GFF1298 at 154.977 kb on + strand, within GFF1298 at 154.977 kb on + strand, within GFF1298 at 155.160 kb on + strand, within GFF1298 at 155.160 kb on + strand, within GFF1298 at 155.160 kb on + strand, within GFF1298 at 155.161 kb on - strand, within GFF1298 at 155.161 kb on - strand, within GFF1298 at 155.161 kb on - strand, within GFF1298 at 155.161 kb on - strand, within GFF1298 at 155.236 kb on - strand, within GFF1298 at 155.236 kb on - strand, within GFF1298 at 155.236 kb on - strand, within GFF1298 at 155.457 kb on + strand, within GFF1298 at 155.458 kb on - strand, within GFF1298 at 155.458 kb on - strand, within GFF1298 at 155.656 kb on + strand at 155.656 kb on + strand at 155.657 kb on - strand at 155.657 kb on - strand at 155.657 kb on - strand at 155.668 kb on + strand at 155.668 kb on + strand at 156.061 kb on + strand, within GFF1300 at 156.062 kb on - strand, within GFF1300 at 156.062 kb on - strand, within GFF1300 at 156.448 kb on + strand, within GFF1300 at 156.448 kb on + strand, within GFF1300 at 156.448 kb on + strand, within GFF1300 at 156.449 kb on - strand, within GFF1300 at 156.449 kb on - strand, within GFF1300 at 156.449 kb on - strand, within GFF1300
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2 remove 154,882 - GFF1298 0.15 +0.6 154,882 - GFF1298 0.15 +0.5 154,882 - GFF1298 0.15 -0.1 154,882 - GFF1298 0.15 -1.3 154,977 + GFF1298 0.27 -0.7 154,977 + GFF1298 0.27 -0.7 155,160 + GFF1298 0.50 -0.4 155,160 + GFF1298 0.50 -0.4 155,160 + GFF1298 0.50 -1.2 155,161 - GFF1298 0.50 +0.5 155,161 - GFF1298 0.50 +0.1 155,161 - GFF1298 0.50 +0.1 155,161 - GFF1298 0.50 -0.5 155,236 - GFF1298 0.60 +0.0 155,236 - GFF1298 0.60 +0.6 155,236 - GFF1298 0.60 +0.8 155,457 + GFF1298 0.88 -0.9 155,458 - GFF1298 0.88 -0.9 155,458 - GFF1298 0.88 -0.3 155,656 + +0.5 155,656 + +0.6 155,657 - +0.5 155,657 - -1.8 155,657 - +0.3 155,668 + +0.1 155,668 + +1.1 156,061 + GFF1300 0.30 -0.3 156,062 - GFF1300 0.30 +0.2 156,062 - GFF1300 0.30 +0.2 156,448 + GFF1300 0.63 -0.0 156,448 + GFF1300 0.63 +0.3 156,448 + GFF1300 0.63 -0.3 156,449 - GFF1300 0.64 -0.7 156,449 - GFF1300 0.64 -0.3 156,449 - GFF1300 0.64 +0.0
Or see this region's nucleotide sequence