Experiment: Supernatant; Pseudomonas DC3000 grown in LB; 0.5 X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliD and COO64_RS21320 are separated by 76 nucleotides COO64_RS21320 and COO64_RS21325 are separated by 71 nucleotides COO64_RS21325 and COO64_RS21330 are separated by 445 nucleotides
COO64_RS21315: fliD - flagellar filament capping protein FliD, at 20,743 to 22,149
fliD
COO64_RS21320: COO64_RS21320 - flagellar protein FlaG, at 22,226 to 22,591
_RS21320
COO64_RS21325: COO64_RS21325 - flagellin domain-containing protein, at 22,663 to 23,511
_RS21325
COO64_RS21330: COO64_RS21330 - ketoacyl-ACP synthase III, at 23,957 to 24,886
_RS21330
Position (kb)
22
23
24 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 21.848 kb on + strand, within fliD at 21.849 kb on - strand, within fliD at 21.849 kb on - strand, within fliD at 21.849 kb on - strand, within fliD at 21.868 kb on - strand, within fliD at 21.868 kb on - strand, within fliD at 21.917 kb on + strand, within fliD at 21.917 kb on + strand, within fliD at 21.917 kb on + strand, within fliD at 21.918 kb on - strand, within fliD at 21.971 kb on + strand, within fliD at 22.105 kb on + strand at 22.106 kb on - strand at 22.141 kb on - strand at 22.149 kb on + strand at 22.149 kb on + strand at 22.149 kb on + strand at 22.150 kb on - strand at 22.153 kb on + strand at 22.153 kb on + strand at 22.153 kb on + strand at 22.153 kb on + strand at 22.153 kb on + strand at 22.154 kb on - strand at 22.154 kb on - strand at 22.154 kb on - strand at 22.154 kb on - strand at 22.154 kb on - strand at 22.154 kb on - strand at 22.167 kb on + strand at 22.256 kb on + strand at 22.256 kb on + strand at 22.256 kb on + strand at 22.256 kb on + strand at 22.256 kb on + strand at 22.256 kb on + strand at 22.257 kb on - strand at 22.257 kb on - strand at 22.342 kb on + strand, within COO64_RS21320 at 22.342 kb on + strand, within COO64_RS21320 at 22.343 kb on - strand, within COO64_RS21320 at 22.343 kb on - strand, within COO64_RS21320 at 22.343 kb on - strand, within COO64_RS21320 at 22.343 kb on - strand, within COO64_RS21320 at 22.343 kb on - strand, within COO64_RS21320 at 22.346 kb on + strand, within COO64_RS21320 at 22.346 kb on + strand, within COO64_RS21320 at 22.347 kb on - strand, within COO64_RS21320 at 22.347 kb on - strand, within COO64_RS21320 at 22.347 kb on - strand, within COO64_RS21320 at 22.388 kb on - strand, within COO64_RS21320 at 22.388 kb on - strand, within COO64_RS21320 at 22.388 kb on - strand, within COO64_RS21320 at 22.388 kb on - strand, within COO64_RS21320 at 22.561 kb on - strand at 22.585 kb on + strand at 22.585 kb on + strand at 22.586 kb on - strand at 22.629 kb on + strand at 22.630 kb on - strand at 22.630 kb on - strand at 22.630 kb on - strand at 22.656 kb on + strand at 22.656 kb on + strand at 22.782 kb on + strand, within COO64_RS21325 at 22.782 kb on + strand, within COO64_RS21325 at 22.783 kb on - strand, within COO64_RS21325 at 22.783 kb on - strand, within COO64_RS21325 at 22.789 kb on + strand, within COO64_RS21325 at 22.789 kb on + strand, within COO64_RS21325 at 22.798 kb on - strand, within COO64_RS21325 at 22.854 kb on + strand, within COO64_RS21325 at 22.854 kb on + strand, within COO64_RS21325 at 22.855 kb on - strand, within COO64_RS21325 at 22.855 kb on - strand, within COO64_RS21325 at 22.855 kb on - strand, within COO64_RS21325 at 22.865 kb on - strand, within COO64_RS21325 at 22.865 kb on - strand, within COO64_RS21325 at 22.865 kb on - strand, within COO64_RS21325 at 22.865 kb on - strand, within COO64_RS21325 at 22.905 kb on + strand, within COO64_RS21325 at 23.017 kb on - strand, within COO64_RS21325 at 23.017 kb on - strand, within COO64_RS21325 at 23.184 kb on + strand, within COO64_RS21325 at 23.184 kb on + strand, within COO64_RS21325 at 23.238 kb on + strand, within COO64_RS21325 at 23.238 kb on + strand, within COO64_RS21325 at 23.238 kb on + strand, within COO64_RS21325 at 23.238 kb on + strand, within COO64_RS21325 at 23.239 kb on - strand, within COO64_RS21325 at 23.298 kb on + strand, within COO64_RS21325 at 23.299 kb on - strand, within COO64_RS21325 at 23.299 kb on - strand, within COO64_RS21325 at 23.299 kb on - strand, within COO64_RS21325 at 23.353 kb on - strand, within COO64_RS21325 at 23.353 kb on - strand, within COO64_RS21325 at 23.484 kb on + strand at 23.484 kb on + strand at 23.484 kb on + strand at 23.484 kb on + strand at 23.484 kb on + strand at 23.484 kb on + strand at 23.484 kb on + strand at 23.484 kb on + strand at 23.484 kb on + strand at 23.484 kb on + strand at 23.484 kb on + strand at 23.485 kb on - strand at 23.485 kb on - strand at 23.490 kb on + strand at 23.490 kb on + strand at 23.490 kb on + strand at 23.490 kb on + strand at 23.535 kb on - strand at 23.573 kb on + strand at 23.574 kb on - strand at 23.574 kb on - strand at 23.574 kb on - strand at 23.586 kb on + strand at 23.586 kb on + strand at 23.587 kb on - strand at 23.587 kb on - strand at 23.589 kb on + strand at 23.589 kb on + strand at 23.589 kb on + strand at 23.589 kb on + strand at 23.589 kb on + strand at 23.590 kb on - strand at 23.590 kb on - strand at 23.590 kb on - strand at 23.590 kb on - strand at 23.593 kb on + strand at 23.593 kb on + strand at 23.593 kb on + strand at 23.593 kb on + strand at 23.593 kb on + strand at 23.593 kb on + strand at 23.593 kb on + strand at 23.594 kb on - strand at 23.594 kb on - strand at 23.594 kb on - strand at 23.594 kb on - strand at 23.693 kb on + strand at 23.693 kb on + strand at 23.745 kb on + strand at 23.745 kb on + strand at 23.835 kb on + strand at 23.835 kb on + strand at 23.835 kb on + strand at 23.836 kb on - strand at 23.836 kb on - strand at 23.888 kb on + strand at 23.953 kb on + strand at 23.953 kb on + strand at 23.953 kb on + strand at 23.953 kb on + strand at 23.953 kb on + strand at 23.953 kb on + strand at 23.954 kb on - strand at 23.954 kb on - strand at 23.954 kb on - strand at 23.954 kb on - strand at 23.954 kb on - strand at 23.954 kb on - strand at 23.955 kb on + strand at 23.955 kb on + strand at 23.956 kb on - strand at 23.956 kb on - strand at 23.956 kb on - strand at 23.956 kb on - strand at 23.956 kb on - strand at 23.956 kb on - strand at 24.085 kb on + strand, within COO64_RS21330 at 24.086 kb on - strand, within COO64_RS21330 at 24.086 kb on - strand, within COO64_RS21330 at 24.086 kb on - strand, within COO64_RS21330 at 24.086 kb on - strand, within COO64_RS21330 at 24.086 kb on - strand, within COO64_RS21330 at 24.088 kb on + strand, within COO64_RS21330 at 24.457 kb on - strand, within COO64_RS21330 at 24.457 kb on - strand, within COO64_RS21330 at 24.457 kb on - strand, within COO64_RS21330
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Pseudomonas DC3000 grown in LB; 0.5 X remove 21,848 + fliD COO64_RS21315 0.79 +1.3 21,849 - fliD COO64_RS21315 0.79 +0.7 21,849 - fliD COO64_RS21315 0.79 +0.3 21,849 - fliD COO64_RS21315 0.79 +1.2 21,868 - fliD COO64_RS21315 0.80 +1.7 21,868 - fliD COO64_RS21315 0.80 +0.2 21,917 + fliD COO64_RS21315 0.83 +0.6 21,917 + fliD COO64_RS21315 0.83 +1.0 21,917 + fliD COO64_RS21315 0.83 +1.8 21,918 - fliD COO64_RS21315 0.84 +1.8 21,971 + fliD COO64_RS21315 0.87 +0.5 22,105 + +1.5 22,106 - +1.1 22,141 - +0.7 22,149 + +1.1 22,149 + +0.3 22,149 + +0.5 22,150 - +2.0 22,153 + +1.6 22,153 + +1.7 22,153 + +1.0 22,153 + +0.4 22,153 + +0.8 22,154 - +0.7 22,154 - -0.4 22,154 - -0.5 22,154 - -0.0 22,154 - +0.8 22,154 - -0.7 22,167 + +1.1 22,256 + -0.9 22,256 + +0.8 22,256 + -1.5 22,256 + -1.8 22,256 + +0.9 22,256 + -0.2 22,257 - -0.0 22,257 - +0.6 22,342 + COO64_RS21320 0.32 +2.1 22,342 + COO64_RS21320 0.32 +0.2 22,343 - COO64_RS21320 0.32 -2.0 22,343 - COO64_RS21320 0.32 -0.3 22,343 - COO64_RS21320 0.32 +0.8 22,343 - COO64_RS21320 0.32 +0.6 22,343 - COO64_RS21320 0.32 +0.1 22,346 + COO64_RS21320 0.33 -0.2 22,346 + COO64_RS21320 0.33 +0.6 22,347 - COO64_RS21320 0.33 -0.2 22,347 - COO64_RS21320 0.33 +0.4 22,347 - COO64_RS21320 0.33 +1.5 22,388 - COO64_RS21320 0.44 +0.1 22,388 - COO64_RS21320 0.44 -1.7 22,388 - COO64_RS21320 0.44 -0.5 22,388 - COO64_RS21320 0.44 +1.8 22,561 - -1.7 22,585 + +1.4 22,585 + -0.8 22,586 - +0.2 22,629 + +1.4 22,630 - +0.1 22,630 - +0.3 22,630 - +0.8 22,656 + +2.1 22,656 + +1.1 22,782 + COO64_RS21325 0.14 +0.8 22,782 + COO64_RS21325 0.14 +0.9 22,783 - COO64_RS21325 0.14 +1.0 22,783 - COO64_RS21325 0.14 +0.9 22,789 + COO64_RS21325 0.15 +3.0 22,789 + COO64_RS21325 0.15 +1.8 22,798 - COO64_RS21325 0.16 +0.9 22,854 + COO64_RS21325 0.22 +0.1 22,854 + COO64_RS21325 0.22 -0.2 22,855 - COO64_RS21325 0.23 +3.0 22,855 - COO64_RS21325 0.23 +1.1 22,855 - COO64_RS21325 0.23 +1.4 22,865 - COO64_RS21325 0.24 +0.8 22,865 - COO64_RS21325 0.24 +1.4 22,865 - COO64_RS21325 0.24 +2.0 22,865 - COO64_RS21325 0.24 +0.2 22,905 + COO64_RS21325 0.29 +1.4 23,017 - COO64_RS21325 0.42 +1.0 23,017 - COO64_RS21325 0.42 -0.3 23,184 + COO64_RS21325 0.61 +1.4 23,184 + COO64_RS21325 0.61 +1.2 23,238 + COO64_RS21325 0.68 +1.4 23,238 + COO64_RS21325 0.68 +1.4 23,238 + COO64_RS21325 0.68 -0.1 23,238 + COO64_RS21325 0.68 -0.2 23,239 - COO64_RS21325 0.68 +0.8 23,298 + COO64_RS21325 0.75 +1.2 23,299 - COO64_RS21325 0.75 +0.2 23,299 - COO64_RS21325 0.75 +2.0 23,299 - COO64_RS21325 0.75 +0.8 23,353 - COO64_RS21325 0.81 +0.8 23,353 - COO64_RS21325 0.81 +0.9 23,484 + +1.1 23,484 + +0.9 23,484 + +1.0 23,484 + +1.2 23,484 + +1.1 23,484 + +1.1 23,484 + +0.5 23,484 + +1.0 23,484 + +0.8 23,484 + +0.7 23,484 + +1.2 23,485 - +1.5 23,485 - +0.8 23,490 + +1.3 23,490 + +0.8 23,490 + +0.7 23,490 + +1.7 23,535 - +0.2 23,573 + +1.1 23,574 - -0.3 23,574 - +0.8 23,574 - +0.9 23,586 + +1.2 23,586 + +1.0 23,587 - +0.2 23,587 - +1.2 23,589 + +1.1 23,589 + +1.6 23,589 + +0.8 23,589 + +1.1 23,589 + +1.6 23,590 - +1.1 23,590 - -0.5 23,590 - +1.4 23,590 - +0.7 23,593 + +1.6 23,593 + +1.8 23,593 + +1.0 23,593 + +0.8 23,593 + +1.4 23,593 + +0.5 23,593 + +1.5 23,594 - +1.0 23,594 - +2.3 23,594 - +1.2 23,594 - +1.1 23,693 + -0.9 23,693 + +0.7 23,745 + +0.4 23,745 + +0.1 23,835 + +0.1 23,835 + +0.0 23,835 + -3.5 23,836 - -0.0 23,836 - +0.1 23,888 + -0.5 23,953 + +0.2 23,953 + -0.0 23,953 + -0.3 23,953 + +0.4 23,953 + +0.4 23,953 + -0.2 23,954 - +0.4 23,954 - +0.3 23,954 - +0.1 23,954 - -0.3 23,954 - -0.1 23,954 - -0.4 23,955 + +0.4 23,955 + +0.8 23,956 - -0.5 23,956 - -0.1 23,956 - -0.4 23,956 - +0.7 23,956 - +0.7 23,956 - -0.7 24,085 + COO64_RS21330 0.14 -0.6 24,086 - COO64_RS21330 0.14 -0.4 24,086 - COO64_RS21330 0.14 -0.2 24,086 - COO64_RS21330 0.14 -0.2 24,086 - COO64_RS21330 0.14 -1.3 24,086 - COO64_RS21330 0.14 -0.4 24,088 + COO64_RS21330 0.14 -0.3 24,457 - COO64_RS21330 0.54 +0.2 24,457 - COO64_RS21330 0.54 -0.5 24,457 - COO64_RS21330 0.54 -0.9
Or see this region's nucleotide sequence