Experiment: Supernatant; Pseudomonas DC3000 grown in LB; 0.5 X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nadA and COO64_RS20220 are separated by 69 nucleotides COO64_RS20220 and COO64_RS20225 are separated by 124 nucleotides COO64_RS20225 and COO64_RS20230 are separated by 24 nucleotides
COO64_RS20215: nadA - quinolinate synthase NadA, at 147,345 to 148,403
nadA
COO64_RS20220: COO64_RS20220 - M48 family metalloprotease, at 148,473 to 149,906
_RS20220
COO64_RS20225: COO64_RS20225 - sulfurtransferase TusA family protein, at 150,031 to 150,270
_RS20225
COO64_RS20230: COO64_RS20230 - AI-2E family transporter, at 150,295 to 151,365
_RS20230
Position (kb) 148
149
150 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 147.489 kb on + strand, within nadA at 147.489 kb on + strand, within nadA at 147.489 kb on + strand, within nadA at 147.489 kb on + strand, within nadA at 147.489 kb on + strand, within nadA at 147.489 kb on + strand, within nadA at 147.490 kb on - strand, within nadA at 147.490 kb on - strand, within nadA at 147.490 kb on - strand, within nadA at 147.492 kb on + strand, within nadA at 147.492 kb on + strand, within nadA at 147.492 kb on + strand, within nadA at 147.492 kb on + strand, within nadA at 147.762 kb on + strand, within nadA at 147.762 kb on + strand, within nadA at 147.762 kb on + strand, within nadA at 147.762 kb on + strand, within nadA at 147.762 kb on + strand, within nadA at 147.762 kb on + strand, within nadA at 147.762 kb on + strand, within nadA at 147.763 kb on - strand, within nadA at 147.763 kb on - strand, within nadA at 147.763 kb on - strand, within nadA at 147.763 kb on - strand, within nadA at 147.897 kb on + strand, within nadA at 147.897 kb on + strand, within nadA at 147.897 kb on + strand, within nadA at 147.898 kb on - strand, within nadA at 147.898 kb on - strand, within nadA at 147.898 kb on - strand, within nadA at 147.898 kb on - strand, within nadA at 148.149 kb on + strand, within nadA at 148.149 kb on + strand, within nadA at 148.149 kb on + strand, within nadA at 148.150 kb on - strand, within nadA at 148.150 kb on - strand, within nadA at 148.352 kb on + strand at 148.352 kb on + strand at 148.352 kb on + strand at 148.352 kb on + strand at 148.352 kb on + strand at 148.352 kb on + strand at 148.352 kb on + strand at 148.353 kb on - strand at 148.353 kb on - strand at 148.353 kb on - strand at 148.353 kb on - strand at 148.433 kb on - strand at 148.433 kb on - strand at 148.438 kb on - strand at 148.438 kb on - strand at 148.438 kb on - strand at 148.574 kb on + strand at 148.627 kb on + strand, within COO64_RS20220 at 148.627 kb on + strand, within COO64_RS20220 at 148.628 kb on - strand, within COO64_RS20220 at 148.693 kb on + strand, within COO64_RS20220 at 148.807 kb on + strand, within COO64_RS20220 at 148.807 kb on + strand, within COO64_RS20220 at 148.808 kb on - strand, within COO64_RS20220 at 148.808 kb on - strand, within COO64_RS20220 at 148.903 kb on + strand, within COO64_RS20220 at 148.903 kb on + strand, within COO64_RS20220 at 148.903 kb on + strand, within COO64_RS20220 at 148.903 kb on + strand, within COO64_RS20220 at 148.903 kb on + strand, within COO64_RS20220 at 148.903 kb on + strand, within COO64_RS20220 at 148.903 kb on + strand, within COO64_RS20220 at 148.904 kb on - strand, within COO64_RS20220 at 148.904 kb on - strand, within COO64_RS20220 at 148.904 kb on - strand, within COO64_RS20220 at 148.904 kb on - strand, within COO64_RS20220 at 148.904 kb on - strand, within COO64_RS20220 at 148.904 kb on - strand, within COO64_RS20220 at 149.077 kb on + strand, within COO64_RS20220 at 149.077 kb on + strand, within COO64_RS20220 at 149.077 kb on + strand, within COO64_RS20220 at 149.077 kb on + strand, within COO64_RS20220 at 149.077 kb on + strand, within COO64_RS20220 at 149.078 kb on - strand, within COO64_RS20220 at 149.078 kb on - strand, within COO64_RS20220 at 149.078 kb on - strand, within COO64_RS20220 at 149.078 kb on - strand, within COO64_RS20220 at 149.227 kb on + strand, within COO64_RS20220 at 149.228 kb on - strand, within COO64_RS20220 at 149.228 kb on - strand, within COO64_RS20220 at 149.234 kb on - strand, within COO64_RS20220 at 149.535 kb on + strand, within COO64_RS20220 at 149.545 kb on + strand, within COO64_RS20220 at 149.545 kb on + strand, within COO64_RS20220 at 149.545 kb on + strand, within COO64_RS20220 at 149.545 kb on + strand, within COO64_RS20220 at 149.545 kb on + strand, within COO64_RS20220 at 149.546 kb on - strand, within COO64_RS20220 at 149.546 kb on - strand, within COO64_RS20220 at 149.546 kb on - strand, within COO64_RS20220 at 149.546 kb on - strand, within COO64_RS20220 at 149.546 kb on - strand, within COO64_RS20220 at 149.546 kb on - strand, within COO64_RS20220 at 149.667 kb on + strand, within COO64_RS20220 at 149.667 kb on + strand, within COO64_RS20220 at 149.668 kb on - strand, within COO64_RS20220 at 149.710 kb on + strand, within COO64_RS20220 at 149.711 kb on - strand, within COO64_RS20220 at 149.935 kb on + strand at 149.935 kb on + strand at 149.935 kb on + strand at 149.936 kb on - strand at 149.936 kb on - strand at 149.936 kb on - strand at 149.936 kb on - strand at 149.936 kb on - strand at 149.936 kb on - strand at 149.999 kb on - strand at 149.999 kb on - strand at 150.210 kb on + strand, within COO64_RS20225 at 150.210 kb on + strand, within COO64_RS20225 at 150.210 kb on + strand, within COO64_RS20225 at 150.210 kb on + strand, within COO64_RS20225 at 150.211 kb on - strand, within COO64_RS20225 at 150.211 kb on - strand, within COO64_RS20225 at 150.211 kb on - strand, within COO64_RS20225 at 150.211 kb on - strand, within COO64_RS20225 at 150.211 kb on - strand, within COO64_RS20225 at 150.211 kb on - strand, within COO64_RS20225 at 150.211 kb on - strand, within COO64_RS20225 at 150.211 kb on - strand, within COO64_RS20225 at 150.211 kb on - strand, within COO64_RS20225 at 150.211 kb on - strand, within COO64_RS20225 at 150.244 kb on + strand, within COO64_RS20225 at 150.244 kb on + strand, within COO64_RS20225 at 150.244 kb on + strand, within COO64_RS20225 at 150.244 kb on + strand, within COO64_RS20225 at 150.244 kb on + strand, within COO64_RS20225 at 150.244 kb on + strand, within COO64_RS20225 at 150.244 kb on + strand, within COO64_RS20225 at 150.244 kb on + strand, within COO64_RS20225 at 150.244 kb on + strand, within COO64_RS20225 at 150.244 kb on + strand, within COO64_RS20225 at 150.244 kb on + strand, within COO64_RS20225 at 150.245 kb on - strand, within COO64_RS20225 at 150.245 kb on - strand, within COO64_RS20225 at 150.245 kb on - strand, within COO64_RS20225 at 150.245 kb on - strand, within COO64_RS20225 at 150.245 kb on - strand, within COO64_RS20225 at 150.245 kb on - strand, within COO64_RS20225 at 150.245 kb on - strand, within COO64_RS20225 at 150.245 kb on - strand, within COO64_RS20225 at 150.245 kb on - strand, within COO64_RS20225 at 150.250 kb on + strand at 150.250 kb on + strand at 150.250 kb on + strand at 150.250 kb on + strand at 150.250 kb on + strand at 150.250 kb on + strand at 150.250 kb on + strand at 150.250 kb on + strand at 150.250 kb on + strand at 150.251 kb on - strand at 150.251 kb on - strand at 150.251 kb on - strand at 150.251 kb on - strand at 150.251 kb on - strand at 150.328 kb on + strand at 150.328 kb on + strand at 150.328 kb on + strand at 150.329 kb on - strand at 150.329 kb on - strand at 150.353 kb on + strand at 150.354 kb on - strand at 150.787 kb on + strand, within COO64_RS20230 at 150.787 kb on + strand, within COO64_RS20230 at 150.787 kb on + strand, within COO64_RS20230 at 150.788 kb on - strand, within COO64_RS20230 at 150.788 kb on - strand, within COO64_RS20230 at 150.788 kb on - strand, within COO64_RS20230 at 150.788 kb on - strand, within COO64_RS20230 at 150.788 kb on - strand, within COO64_RS20230 at 150.788 kb on - strand, within COO64_RS20230 at 150.788 kb on - strand, within COO64_RS20230 at 150.788 kb on - strand, within COO64_RS20230 at 150.862 kb on + strand, within COO64_RS20230
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Pseudomonas DC3000 grown in LB; 0.5 X remove 147,489 + nadA COO64_RS20215 0.14 -0.7 147,489 + nadA COO64_RS20215 0.14 -2.0 147,489 + nadA COO64_RS20215 0.14 -0.4 147,489 + nadA COO64_RS20215 0.14 -0.3 147,489 + nadA COO64_RS20215 0.14 -0.4 147,489 + nadA COO64_RS20215 0.14 -1.1 147,490 - nadA COO64_RS20215 0.14 -1.2 147,490 - nadA COO64_RS20215 0.14 -0.6 147,490 - nadA COO64_RS20215 0.14 -1.1 147,492 + nadA COO64_RS20215 0.14 +0.4 147,492 + nadA COO64_RS20215 0.14 +0.2 147,492 + nadA COO64_RS20215 0.14 -0.7 147,492 + nadA COO64_RS20215 0.14 +0.5 147,762 + nadA COO64_RS20215 0.39 +0.8 147,762 + nadA COO64_RS20215 0.39 -0.0 147,762 + nadA COO64_RS20215 0.39 +0.5 147,762 + nadA COO64_RS20215 0.39 -0.7 147,762 + nadA COO64_RS20215 0.39 -0.1 147,762 + nadA COO64_RS20215 0.39 +0.4 147,762 + nadA COO64_RS20215 0.39 -0.2 147,763 - nadA COO64_RS20215 0.39 -0.2 147,763 - nadA COO64_RS20215 0.39 +0.1 147,763 - nadA COO64_RS20215 0.39 -0.2 147,763 - nadA COO64_RS20215 0.39 +0.4 147,897 + nadA COO64_RS20215 0.52 -1.1 147,897 + nadA COO64_RS20215 0.52 +0.1 147,897 + nadA COO64_RS20215 0.52 -0.1 147,898 - nadA COO64_RS20215 0.52 +0.4 147,898 - nadA COO64_RS20215 0.52 +0.6 147,898 - nadA COO64_RS20215 0.52 -1.1 147,898 - nadA COO64_RS20215 0.52 +0.3 148,149 + nadA COO64_RS20215 0.76 -0.3 148,149 + nadA COO64_RS20215 0.76 +0.6 148,149 + nadA COO64_RS20215 0.76 -0.6 148,150 - nadA COO64_RS20215 0.76 +0.3 148,150 - nadA COO64_RS20215 0.76 +0.8 148,352 + +1.5 148,352 + -0.3 148,352 + -0.6 148,352 + +0.0 148,352 + -1.1 148,352 + -0.3 148,352 + +0.1 148,353 - +0.5 148,353 - -0.2 148,353 - +0.1 148,353 - +0.9 148,433 - -0.1 148,433 - -0.6 148,438 - -0.6 148,438 - -0.6 148,438 - -0.3 148,574 + -0.2 148,627 + COO64_RS20220 0.11 -0.3 148,627 + COO64_RS20220 0.11 -0.4 148,628 - COO64_RS20220 0.11 -0.3 148,693 + COO64_RS20220 0.15 +0.4 148,807 + COO64_RS20220 0.23 -0.4 148,807 + COO64_RS20220 0.23 -0.3 148,808 - COO64_RS20220 0.23 -0.9 148,808 - COO64_RS20220 0.23 -0.2 148,903 + COO64_RS20220 0.30 -0.0 148,903 + COO64_RS20220 0.30 -0.4 148,903 + COO64_RS20220 0.30 +0.6 148,903 + COO64_RS20220 0.30 -1.0 148,903 + COO64_RS20220 0.30 +0.1 148,903 + COO64_RS20220 0.30 -0.5 148,903 + COO64_RS20220 0.30 +0.1 148,904 - COO64_RS20220 0.30 -0.7 148,904 - COO64_RS20220 0.30 +0.0 148,904 - COO64_RS20220 0.30 -0.1 148,904 - COO64_RS20220 0.30 -0.1 148,904 - COO64_RS20220 0.30 -0.9 148,904 - COO64_RS20220 0.30 -1.2 149,077 + COO64_RS20220 0.42 -1.7 149,077 + COO64_RS20220 0.42 +0.2 149,077 + COO64_RS20220 0.42 -1.4 149,077 + COO64_RS20220 0.42 -1.3 149,077 + COO64_RS20220 0.42 +0.1 149,078 - COO64_RS20220 0.42 -1.3 149,078 - COO64_RS20220 0.42 +0.3 149,078 - COO64_RS20220 0.42 +0.0 149,078 - COO64_RS20220 0.42 +0.5 149,227 + COO64_RS20220 0.53 -0.5 149,228 - COO64_RS20220 0.53 +0.1 149,228 - COO64_RS20220 0.53 -0.6 149,234 - COO64_RS20220 0.53 -0.5 149,535 + COO64_RS20220 0.74 +1.0 149,545 + COO64_RS20220 0.75 +1.6 149,545 + COO64_RS20220 0.75 -0.1 149,545 + COO64_RS20220 0.75 +0.4 149,545 + COO64_RS20220 0.75 -0.5 149,545 + COO64_RS20220 0.75 -0.2 149,546 - COO64_RS20220 0.75 +0.3 149,546 - COO64_RS20220 0.75 +0.5 149,546 - COO64_RS20220 0.75 +0.4 149,546 - COO64_RS20220 0.75 -0.4 149,546 - COO64_RS20220 0.75 -0.5 149,546 - COO64_RS20220 0.75 +0.0 149,667 + COO64_RS20220 0.83 -0.3 149,667 + COO64_RS20220 0.83 -0.6 149,668 - COO64_RS20220 0.83 -0.3 149,710 + COO64_RS20220 0.86 +0.3 149,711 - COO64_RS20220 0.86 -0.4 149,935 + +0.2 149,935 + +0.2 149,935 + +0.2 149,936 - -1.0 149,936 - +0.2 149,936 - -1.1 149,936 - +0.2 149,936 - +0.4 149,936 - -0.8 149,999 - -0.3 149,999 - +0.2 150,210 + COO64_RS20225 0.75 -1.4 150,210 + COO64_RS20225 0.75 -0.9 150,210 + COO64_RS20225 0.75 -1.1 150,210 + COO64_RS20225 0.75 +0.5 150,211 - COO64_RS20225 0.75 +0.6 150,211 - COO64_RS20225 0.75 -1.4 150,211 - COO64_RS20225 0.75 +0.3 150,211 - COO64_RS20225 0.75 +0.6 150,211 - COO64_RS20225 0.75 -1.0 150,211 - COO64_RS20225 0.75 -0.1 150,211 - COO64_RS20225 0.75 -0.4 150,211 - COO64_RS20225 0.75 -1.0 150,211 - COO64_RS20225 0.75 +0.6 150,211 - COO64_RS20225 0.75 +0.1 150,244 + COO64_RS20225 0.89 -1.0 150,244 + COO64_RS20225 0.89 -0.3 150,244 + COO64_RS20225 0.89 -0.2 150,244 + COO64_RS20225 0.89 -2.7 150,244 + COO64_RS20225 0.89 -0.7 150,244 + COO64_RS20225 0.89 -0.1 150,244 + COO64_RS20225 0.89 +1.4 150,244 + COO64_RS20225 0.89 -0.9 150,244 + COO64_RS20225 0.89 -0.7 150,244 + COO64_RS20225 0.89 +0.2 150,244 + COO64_RS20225 0.89 -1.3 150,245 - COO64_RS20225 0.89 -0.1 150,245 - COO64_RS20225 0.89 -0.9 150,245 - COO64_RS20225 0.89 -0.4 150,245 - COO64_RS20225 0.89 +1.3 150,245 - COO64_RS20225 0.89 +0.0 150,245 - COO64_RS20225 0.89 -1.6 150,245 - COO64_RS20225 0.89 -0.6 150,245 - COO64_RS20225 0.89 +0.0 150,245 - COO64_RS20225 0.89 -0.8 150,250 + -0.0 150,250 + -2.8 150,250 + +0.3 150,250 + -0.7 150,250 + +0.8 150,250 + -0.9 150,250 + -1.2 150,250 + -0.2 150,250 + +1.5 150,251 - -1.8 150,251 - -0.9 150,251 - -0.6 150,251 - +0.4 150,251 - +0.1 150,328 + -0.1 150,328 + +0.1 150,328 + -0.9 150,329 - -0.2 150,329 - -0.8 150,353 + +0.3 150,354 - -0.2 150,787 + COO64_RS20230 0.46 +1.6 150,787 + COO64_RS20230 0.46 +0.2 150,787 + COO64_RS20230 0.46 -0.6 150,788 - COO64_RS20230 0.46 -1.3 150,788 - COO64_RS20230 0.46 +0.1 150,788 - COO64_RS20230 0.46 -0.5 150,788 - COO64_RS20230 0.46 +1.0 150,788 - COO64_RS20230 0.46 -0.5 150,788 - COO64_RS20230 0.46 +0.9 150,788 - COO64_RS20230 0.46 +0.5 150,788 - COO64_RS20230 0.46 -1.8 150,862 + COO64_RS20230 0.53 -1.1
Or see this region's nucleotide sequence