Experiment: R2A_PIPES with Metal_ion_mix_sitestressplate 0.5 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_13255 and ubiJ are separated by 122 nucleotides ubiJ and ubiB overlap by 4 nucleotides ubiB and OKGIIK_13270 are separated by 126 nucleotides OKGIIK_13270 and arfB are separated by 96 nucleotides
OKGIIK_13255: OKGIIK_13255 - tRNA (cytidine(34)-2'-O)-methyltransferase, at 2,861,237 to 2,861,704
_13255
OKGIIK_13260: ubiJ - sterol-binding protein, at 2,861,827 to 2,862,477
ubiJ
OKGIIK_13265: ubiB - ubiquinone biosynthesis regulatory protein kinase UbiB, at 2,862,474 to 2,864,123
ubiB
OKGIIK_13270: OKGIIK_13270 - pseudouridylate synthase, at 2,864,250 to 2,864,966
_13270
OKGIIK_13275: arfB - aminoacyl-tRNA hydrolase, at 2,865,063 to 2,865,485
arfB
Position (kb)
2862
2863
2864
2865 Strain fitness (log2 ratio)
-2
-1
0
1 at 2861.661 kb on - strand at 2861.661 kb on - strand at 2861.703 kb on - strand at 2861.749 kb on + strand at 2861.749 kb on + strand at 2861.750 kb on - strand at 2861.793 kb on + strand at 2861.793 kb on + strand at 2861.793 kb on + strand at 2861.793 kb on + strand at 2861.793 kb on + strand at 2861.793 kb on + strand at 2861.793 kb on + strand at 2861.793 kb on + strand at 2861.815 kb on + strand at 2861.815 kb on + strand at 2861.815 kb on + strand at 2861.816 kb on - strand at 2864.149 kb on - strand at 2864.149 kb on - strand at 2864.149 kb on - strand at 2864.391 kb on + strand, within OKGIIK_13270 at 2864.391 kb on + strand, within OKGIIK_13270 at 2864.392 kb on - strand, within OKGIIK_13270 at 2864.505 kb on + strand, within OKGIIK_13270 at 2864.505 kb on + strand, within OKGIIK_13270 at 2864.505 kb on + strand, within OKGIIK_13270 at 2864.506 kb on - strand, within OKGIIK_13270 at 2864.506 kb on - strand, within OKGIIK_13270 at 2864.506 kb on - strand, within OKGIIK_13270 at 2864.506 kb on - strand, within OKGIIK_13270 at 2864.506 kb on - strand, within OKGIIK_13270 at 2864.506 kb on - strand, within OKGIIK_13270 at 2864.556 kb on + strand, within OKGIIK_13270 at 2864.557 kb on - strand, within OKGIIK_13270 at 2864.611 kb on - strand, within OKGIIK_13270 at 2864.793 kb on + strand, within OKGIIK_13270 at 2864.793 kb on + strand, within OKGIIK_13270 at 2864.793 kb on + strand, within OKGIIK_13270 at 2864.793 kb on + strand, within OKGIIK_13270 at 2864.794 kb on - strand, within OKGIIK_13270 at 2864.794 kb on - strand, within OKGIIK_13270 at 2864.794 kb on - strand, within OKGIIK_13270 at 2864.905 kb on - strand at 2865.050 kb on + strand at 2865.050 kb on + strand at 2865.050 kb on + strand at 2865.051 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Metal_ion_mix_sitestressplate 0.5 x remove 2,861,661 - +0.1 2,861,661 - +0.1 2,861,703 - +1.4 2,861,749 + +0.5 2,861,749 + -1.6 2,861,750 - -1.8 2,861,793 + -0.1 2,861,793 + -1.2 2,861,793 + -1.6 2,861,793 + +0.5 2,861,793 + +0.0 2,861,793 + +0.4 2,861,793 + -0.9 2,861,793 + -0.3 2,861,815 + +1.0 2,861,815 + +0.6 2,861,815 + +1.6 2,861,816 - +0.8 2,864,149 - -0.0 2,864,149 - +1.0 2,864,149 - +1.1 2,864,391 + OKGIIK_13270 0.20 -1.5 2,864,391 + OKGIIK_13270 0.20 +0.0 2,864,392 - OKGIIK_13270 0.20 -0.2 2,864,505 + OKGIIK_13270 0.36 +0.4 2,864,505 + OKGIIK_13270 0.36 +0.5 2,864,505 + OKGIIK_13270 0.36 -0.3 2,864,506 - OKGIIK_13270 0.36 +0.4 2,864,506 - OKGIIK_13270 0.36 +1.0 2,864,506 - OKGIIK_13270 0.36 +0.2 2,864,506 - OKGIIK_13270 0.36 -0.5 2,864,506 - OKGIIK_13270 0.36 +0.7 2,864,506 - OKGIIK_13270 0.36 -0.4 2,864,556 + OKGIIK_13270 0.43 +0.6 2,864,557 - OKGIIK_13270 0.43 -1.1 2,864,611 - OKGIIK_13270 0.50 -0.3 2,864,793 + OKGIIK_13270 0.76 +0.2 2,864,793 + OKGIIK_13270 0.76 -0.6 2,864,793 + OKGIIK_13270 0.76 -0.8 2,864,793 + OKGIIK_13270 0.76 +1.2 2,864,794 - OKGIIK_13270 0.76 +0.2 2,864,794 - OKGIIK_13270 0.76 -0.1 2,864,794 - OKGIIK_13270 0.76 -0.0 2,864,905 - +0.7 2,865,050 + -0.9 2,865,050 + -1.4 2,865,050 + -1.8 2,865,051 - -0.2
Or see this region's nucleotide sequence