Experiment: R2A_PIPES with Metal_ion_mix_sitestressplate 0.5 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cusA and OKGIIK_04615 are separated by 3 nucleotides OKGIIK_04615 and OKGIIK_04620 are separated by 11 nucleotides OKGIIK_04620 and OKGIIK_04625 overlap by 20 nucleotides OKGIIK_04625 and rhaT are separated by 34 nucleotides
OKGIIK_04610: cusA - CusA/CzcA family heavy metal efflux RND transporter, at 1,006,517 to 1,009,693
cusA
OKGIIK_04615: OKGIIK_04615 - Copper-binding protein, at 1,009,697 to 1,010,053
_04615
OKGIIK_04620: OKGIIK_04620 - hypothetical protein, at 1,010,065 to 1,010,412
_04620
OKGIIK_04625: OKGIIK_04625 - Methyltransferase, at 1,010,393 to 1,011,037
_04625
OKGIIK_04630: rhaT - EamA family transporter, at 1,011,072 to 1,012,001
rhaT
Position (kb)
1010
1011
1012 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1009.443 kb on + strand at 1009.443 kb on + strand at 1009.443 kb on + strand at 1009.444 kb on - strand at 1009.545 kb on + strand at 1009.546 kb on - strand at 1009.546 kb on - strand at 1009.600 kb on + strand at 1009.600 kb on + strand at 1009.600 kb on + strand at 1009.600 kb on + strand at 1009.601 kb on - strand at 1009.601 kb on - strand at 1009.601 kb on - strand at 1009.601 kb on - strand at 1009.602 kb on + strand at 1009.602 kb on + strand at 1009.602 kb on + strand at 1009.602 kb on + strand at 1009.602 kb on + strand at 1009.603 kb on - strand at 1009.603 kb on - strand at 1009.603 kb on - strand at 1009.603 kb on - strand at 1009.603 kb on - strand at 1009.603 kb on - strand at 1009.603 kb on - strand at 1009.603 kb on - strand at 1009.642 kb on + strand at 1009.705 kb on - strand at 1009.753 kb on - strand, within OKGIIK_04615 at 1009.753 kb on - strand, within OKGIIK_04615 at 1009.753 kb on - strand, within OKGIIK_04615 at 1009.842 kb on + strand, within OKGIIK_04615 at 1009.842 kb on + strand, within OKGIIK_04615 at 1009.842 kb on + strand, within OKGIIK_04615 at 1009.842 kb on + strand, within OKGIIK_04615 at 1009.842 kb on + strand, within OKGIIK_04615 at 1009.842 kb on + strand, within OKGIIK_04615 at 1009.843 kb on - strand, within OKGIIK_04615 at 1009.843 kb on - strand, within OKGIIK_04615 at 1009.843 kb on - strand, within OKGIIK_04615 at 1009.843 kb on - strand, within OKGIIK_04615 at 1009.876 kb on + strand, within OKGIIK_04615 at 1009.876 kb on + strand, within OKGIIK_04615 at 1009.876 kb on + strand, within OKGIIK_04615 at 1009.876 kb on + strand, within OKGIIK_04615 at 1009.876 kb on + strand, within OKGIIK_04615 at 1009.876 kb on + strand, within OKGIIK_04615 at 1009.876 kb on + strand, within OKGIIK_04615 at 1009.877 kb on - strand, within OKGIIK_04615 at 1009.953 kb on + strand, within OKGIIK_04615 at 1009.953 kb on + strand, within OKGIIK_04615 at 1009.953 kb on + strand, within OKGIIK_04615 at 1009.954 kb on - strand, within OKGIIK_04615 at 1009.954 kb on - strand, within OKGIIK_04615 at 1009.983 kb on + strand, within OKGIIK_04615 at 1009.983 kb on + strand, within OKGIIK_04615 at 1009.983 kb on + strand, within OKGIIK_04615 at 1009.983 kb on + strand, within OKGIIK_04615 at 1009.983 kb on + strand, within OKGIIK_04615 at 1009.983 kb on + strand, within OKGIIK_04615 at 1009.984 kb on - strand, within OKGIIK_04615 at 1009.984 kb on - strand, within OKGIIK_04615 at 1009.984 kb on - strand, within OKGIIK_04615 at 1010.051 kb on + strand at 1010.052 kb on - strand at 1010.052 kb on - strand at 1010.052 kb on - strand at 1010.052 kb on - strand at 1010.052 kb on - strand at 1010.161 kb on + strand, within OKGIIK_04620 at 1010.161 kb on + strand, within OKGIIK_04620 at 1010.161 kb on + strand, within OKGIIK_04620 at 1010.161 kb on + strand, within OKGIIK_04620 at 1010.161 kb on + strand, within OKGIIK_04620 at 1010.161 kb on + strand, within OKGIIK_04620 at 1010.161 kb on + strand, within OKGIIK_04620 at 1010.161 kb on + strand, within OKGIIK_04620 at 1010.161 kb on + strand, within OKGIIK_04620 at 1010.161 kb on + strand, within OKGIIK_04620 at 1010.324 kb on + strand, within OKGIIK_04620 at 1010.324 kb on + strand, within OKGIIK_04620 at 1010.324 kb on + strand, within OKGIIK_04620 at 1010.325 kb on - strand, within OKGIIK_04620 at 1010.325 kb on - strand, within OKGIIK_04620 at 1010.325 kb on - strand, within OKGIIK_04620 at 1010.442 kb on + strand at 1010.443 kb on - strand at 1010.443 kb on - strand at 1010.443 kb on - strand at 1010.443 kb on - strand at 1010.522 kb on + strand, within OKGIIK_04625 at 1010.547 kb on + strand, within OKGIIK_04625 at 1010.547 kb on + strand, within OKGIIK_04625 at 1010.547 kb on + strand, within OKGIIK_04625 at 1010.558 kb on - strand, within OKGIIK_04625 at 1010.652 kb on + strand, within OKGIIK_04625 at 1010.652 kb on + strand, within OKGIIK_04625 at 1010.653 kb on - strand, within OKGIIK_04625 at 1010.653 kb on - strand, within OKGIIK_04625 at 1010.653 kb on - strand, within OKGIIK_04625 at 1010.672 kb on + strand, within OKGIIK_04625 at 1010.672 kb on + strand, within OKGIIK_04625 at 1010.672 kb on + strand, within OKGIIK_04625 at 1010.672 kb on + strand, within OKGIIK_04625 at 1010.672 kb on + strand, within OKGIIK_04625 at 1010.672 kb on + strand, within OKGIIK_04625 at 1010.673 kb on - strand, within OKGIIK_04625 at 1010.673 kb on - strand, within OKGIIK_04625 at 1010.715 kb on + strand, within OKGIIK_04625 at 1010.716 kb on - strand, within OKGIIK_04625 at 1010.716 kb on - strand, within OKGIIK_04625 at 1010.729 kb on + strand, within OKGIIK_04625 at 1010.953 kb on + strand, within OKGIIK_04625 at 1010.953 kb on + strand, within OKGIIK_04625 at 1010.953 kb on + strand, within OKGIIK_04625 at 1010.954 kb on - strand, within OKGIIK_04625 at 1010.954 kb on - strand, within OKGIIK_04625 at 1011.004 kb on - strand at 1011.193 kb on + strand, within rhaT at 1011.193 kb on + strand, within rhaT at 1011.193 kb on + strand, within rhaT at 1011.194 kb on - strand, within rhaT at 1011.253 kb on + strand, within rhaT at 1011.253 kb on + strand, within rhaT at 1011.253 kb on + strand, within rhaT at 1011.254 kb on - strand, within rhaT at 1011.754 kb on + strand, within rhaT at 1011.754 kb on + strand, within rhaT at 1011.754 kb on + strand, within rhaT at 1011.755 kb on - strand, within rhaT at 1011.755 kb on - strand, within rhaT at 1011.755 kb on - strand, within rhaT at 1011.755 kb on - strand, within rhaT at 1011.777 kb on + strand, within rhaT at 1011.778 kb on - strand, within rhaT at 1011.799 kb on + strand, within rhaT at 1011.799 kb on + strand, within rhaT at 1011.799 kb on + strand, within rhaT at 1011.799 kb on + strand, within rhaT at 1011.799 kb on + strand, within rhaT at 1011.800 kb on - strand, within rhaT at 1011.800 kb on - strand, within rhaT at 1011.800 kb on - strand, within rhaT at 1011.800 kb on - strand, within rhaT at 1011.800 kb on - strand, within rhaT at 1011.800 kb on - strand, within rhaT at 1011.800 kb on - strand, within rhaT at 1011.800 kb on - strand, within rhaT at 1012.030 kb on + strand at 1012.031 kb on - strand at 1012.031 kb on - strand at 1012.031 kb on - strand at 1012.031 kb on - strand at 1012.031 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Metal_ion_mix_sitestressplate 0.5 x remove 1,009,443 + -0.7 1,009,443 + -0.5 1,009,443 + -0.7 1,009,444 - +0.9 1,009,545 + -1.1 1,009,546 - -0.6 1,009,546 - -0.7 1,009,600 + +0.0 1,009,600 + -0.5 1,009,600 + -3.0 1,009,600 + -0.7 1,009,601 - -1.3 1,009,601 - -1.7 1,009,601 - -1.4 1,009,601 - +1.3 1,009,602 + -1.9 1,009,602 + -1.7 1,009,602 + -1.1 1,009,602 + -2.2 1,009,602 + -1.1 1,009,603 - -1.1 1,009,603 - -0.7 1,009,603 - -0.9 1,009,603 - -1.1 1,009,603 - -1.7 1,009,603 - +0.6 1,009,603 - +0.6 1,009,603 - -0.8 1,009,642 + +0.1 1,009,705 - -0.6 1,009,753 - OKGIIK_04615 0.16 +1.3 1,009,753 - OKGIIK_04615 0.16 +0.5 1,009,753 - OKGIIK_04615 0.16 +0.3 1,009,842 + OKGIIK_04615 0.41 -1.0 1,009,842 + OKGIIK_04615 0.41 +0.3 1,009,842 + OKGIIK_04615 0.41 -2.0 1,009,842 + OKGIIK_04615 0.41 -0.9 1,009,842 + OKGIIK_04615 0.41 -0.3 1,009,842 + OKGIIK_04615 0.41 -1.2 1,009,843 - OKGIIK_04615 0.41 -0.4 1,009,843 - OKGIIK_04615 0.41 -2.4 1,009,843 - OKGIIK_04615 0.41 -0.1 1,009,843 - OKGIIK_04615 0.41 -0.5 1,009,876 + OKGIIK_04615 0.50 +0.5 1,009,876 + OKGIIK_04615 0.50 -1.2 1,009,876 + OKGIIK_04615 0.50 -1.2 1,009,876 + OKGIIK_04615 0.50 -1.3 1,009,876 + OKGIIK_04615 0.50 -1.1 1,009,876 + OKGIIK_04615 0.50 +0.4 1,009,876 + OKGIIK_04615 0.50 -0.4 1,009,877 - OKGIIK_04615 0.50 -1.9 1,009,953 + OKGIIK_04615 0.72 -2.4 1,009,953 + OKGIIK_04615 0.72 +1.3 1,009,953 + OKGIIK_04615 0.72 -0.7 1,009,954 - OKGIIK_04615 0.72 -0.1 1,009,954 - OKGIIK_04615 0.72 -1.3 1,009,983 + OKGIIK_04615 0.80 -1.4 1,009,983 + OKGIIK_04615 0.80 -0.7 1,009,983 + OKGIIK_04615 0.80 -0.4 1,009,983 + OKGIIK_04615 0.80 -1.8 1,009,983 + OKGIIK_04615 0.80 +0.5 1,009,983 + OKGIIK_04615 0.80 +0.3 1,009,984 - OKGIIK_04615 0.80 -1.1 1,009,984 - OKGIIK_04615 0.80 -1.5 1,009,984 - OKGIIK_04615 0.80 -1.1 1,010,051 + -0.2 1,010,052 - +0.3 1,010,052 - +0.3 1,010,052 - -0.4 1,010,052 - -0.1 1,010,052 - +0.3 1,010,161 + OKGIIK_04620 0.28 -2.6 1,010,161 + OKGIIK_04620 0.28 +0.1 1,010,161 + OKGIIK_04620 0.28 -0.3 1,010,161 + OKGIIK_04620 0.28 -0.5 1,010,161 + OKGIIK_04620 0.28 -0.2 1,010,161 + OKGIIK_04620 0.28 -2.2 1,010,161 + OKGIIK_04620 0.28 -0.3 1,010,161 + OKGIIK_04620 0.28 -0.2 1,010,161 + OKGIIK_04620 0.28 +0.3 1,010,161 + OKGIIK_04620 0.28 -2.1 1,010,324 + OKGIIK_04620 0.74 -2.1 1,010,324 + OKGIIK_04620 0.74 -1.1 1,010,324 + OKGIIK_04620 0.74 -0.3 1,010,325 - OKGIIK_04620 0.75 -0.7 1,010,325 - OKGIIK_04620 0.75 +0.1 1,010,325 - OKGIIK_04620 0.75 +0.0 1,010,442 + +1.3 1,010,443 - -0.7 1,010,443 - -2.3 1,010,443 - -2.2 1,010,443 - +1.7 1,010,522 + OKGIIK_04625 0.20 -1.3 1,010,547 + OKGIIK_04625 0.24 +0.7 1,010,547 + OKGIIK_04625 0.24 -0.7 1,010,547 + OKGIIK_04625 0.24 -1.3 1,010,558 - OKGIIK_04625 0.26 -0.2 1,010,652 + OKGIIK_04625 0.40 -0.2 1,010,652 + OKGIIK_04625 0.40 -1.1 1,010,653 - OKGIIK_04625 0.40 -1.1 1,010,653 - OKGIIK_04625 0.40 -1.3 1,010,653 - OKGIIK_04625 0.40 +0.5 1,010,672 + OKGIIK_04625 0.43 -0.3 1,010,672 + OKGIIK_04625 0.43 -2.1 1,010,672 + OKGIIK_04625 0.43 -1.3 1,010,672 + OKGIIK_04625 0.43 +0.3 1,010,672 + OKGIIK_04625 0.43 +0.3 1,010,672 + OKGIIK_04625 0.43 -1.1 1,010,673 - OKGIIK_04625 0.43 +0.9 1,010,673 - OKGIIK_04625 0.43 -0.2 1,010,715 + OKGIIK_04625 0.50 -1.7 1,010,716 - OKGIIK_04625 0.50 +0.1 1,010,716 - OKGIIK_04625 0.50 -1.1 1,010,729 + OKGIIK_04625 0.52 +0.7 1,010,953 + OKGIIK_04625 0.87 -0.1 1,010,953 + OKGIIK_04625 0.87 +0.7 1,010,953 + OKGIIK_04625 0.87 -1.9 1,010,954 - OKGIIK_04625 0.87 +0.5 1,010,954 - OKGIIK_04625 0.87 +1.0 1,011,004 - -1.9 1,011,193 + rhaT OKGIIK_04630 0.13 -0.7 1,011,193 + rhaT OKGIIK_04630 0.13 -0.7 1,011,193 + rhaT OKGIIK_04630 0.13 -1.3 1,011,194 - rhaT OKGIIK_04630 0.13 +0.1 1,011,253 + rhaT OKGIIK_04630 0.19 -1.0 1,011,253 + rhaT OKGIIK_04630 0.19 +1.0 1,011,253 + rhaT OKGIIK_04630 0.19 +0.3 1,011,254 - rhaT OKGIIK_04630 0.20 -2.3 1,011,754 + rhaT OKGIIK_04630 0.73 +0.4 1,011,754 + rhaT OKGIIK_04630 0.73 -0.7 1,011,754 + rhaT OKGIIK_04630 0.73 -0.3 1,011,755 - rhaT OKGIIK_04630 0.73 -1.1 1,011,755 - rhaT OKGIIK_04630 0.73 -1.5 1,011,755 - rhaT OKGIIK_04630 0.73 -1.3 1,011,755 - rhaT OKGIIK_04630 0.73 +0.1 1,011,777 + rhaT OKGIIK_04630 0.76 -1.3 1,011,778 - rhaT OKGIIK_04630 0.76 -1.7 1,011,799 + rhaT OKGIIK_04630 0.78 -0.1 1,011,799 + rhaT OKGIIK_04630 0.78 -1.2 1,011,799 + rhaT OKGIIK_04630 0.78 -0.7 1,011,799 + rhaT OKGIIK_04630 0.78 -1.1 1,011,799 + rhaT OKGIIK_04630 0.78 -0.3 1,011,800 - rhaT OKGIIK_04630 0.78 +0.0 1,011,800 - rhaT OKGIIK_04630 0.78 -1.3 1,011,800 - rhaT OKGIIK_04630 0.78 -1.5 1,011,800 - rhaT OKGIIK_04630 0.78 -1.2 1,011,800 - rhaT OKGIIK_04630 0.78 -0.3 1,011,800 - rhaT OKGIIK_04630 0.78 -0.5 1,011,800 - rhaT OKGIIK_04630 0.78 -1.3 1,011,800 - rhaT OKGIIK_04630 0.78 -0.7 1,012,030 + +0.4 1,012,031 - -1.1 1,012,031 - -2.2 1,012,031 - +1.0 1,012,031 - -0.7 1,012,031 - +0.5
Or see this region's nucleotide sequence