Experiment: R2A_PIPES with Metal_ion_mix_sitestressplate 0.5 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ptsP and OKGIIK_02240 are separated by 142 nucleotides OKGIIK_02240 and manX overlap by 8 nucleotides manX and rapZ are separated by 43 nucleotides
OKGIIK_02235: ptsP - phosphoenolpyruvate--protein phosphotransferase, at 525,065 to 526,807
ptsP
OKGIIK_02240: OKGIIK_02240 - Phosphocarrier protein HPr, at 526,950 to 527,219
_02240
OKGIIK_02245: manX - PTS fructose IIA subunit family protein, at 527,212 to 527,604
manX
OKGIIK_02250: rapZ - RNase adapter RapZ, at 527,648 to 528,550
rapZ
Position (kb) 526
527
528 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 526.233 kb on + strand, within ptsP at 526.233 kb on + strand, within ptsP at 526.233 kb on + strand, within ptsP at 526.233 kb on + strand, within ptsP at 526.234 kb on - strand, within ptsP at 526.234 kb on - strand, within ptsP at 526.234 kb on - strand, within ptsP at 526.434 kb on + strand, within ptsP at 526.434 kb on + strand, within ptsP at 526.435 kb on - strand, within ptsP at 526.435 kb on - strand, within ptsP at 526.435 kb on - strand, within ptsP at 526.435 kb on - strand, within ptsP at 526.435 kb on - strand, within ptsP at 526.435 kb on - strand, within ptsP at 526.506 kb on + strand, within ptsP at 526.728 kb on + strand at 526.728 kb on + strand at 526.728 kb on + strand at 526.728 kb on + strand at 526.728 kb on + strand at 526.728 kb on + strand at 526.728 kb on + strand at 526.729 kb on - strand at 526.729 kb on - strand at 526.729 kb on - strand at 526.729 kb on - strand at 526.951 kb on + strand at 526.951 kb on + strand at 526.951 kb on + strand at 526.951 kb on + strand at 526.951 kb on + strand at 526.952 kb on - strand at 526.952 kb on - strand at 526.952 kb on - strand at 526.952 kb on - strand at 527.121 kb on + strand, within OKGIIK_02240 at 527.121 kb on + strand, within OKGIIK_02240 at 527.121 kb on + strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.299 kb on + strand, within manX at 527.299 kb on + strand, within manX at 527.300 kb on - strand, within manX at 527.300 kb on - strand, within manX at 527.300 kb on - strand, within manX at 527.347 kb on + strand, within manX at 527.347 kb on + strand, within manX at 527.347 kb on + strand, within manX at 527.347 kb on + strand, within manX at 527.347 kb on + strand, within manX at 527.347 kb on + strand, within manX at 527.347 kb on + strand, within manX at 527.347 kb on + strand, within manX at 527.347 kb on + strand, within manX at 527.347 kb on + strand, within manX at 527.347 kb on + strand, within manX at 527.347 kb on + strand, within manX at 527.347 kb on + strand, within manX at 527.348 kb on - strand, within manX at 527.348 kb on - strand, within manX at 527.348 kb on - strand, within manX at 527.348 kb on - strand, within manX at 527.348 kb on - strand, within manX at 527.348 kb on - strand, within manX at 527.348 kb on - strand, within manX at 527.348 kb on - strand, within manX at 527.348 kb on - strand, within manX at 527.348 kb on - strand, within manX at 527.348 kb on - strand, within manX at 527.396 kb on + strand, within manX at 527.396 kb on + strand, within manX at 527.396 kb on + strand, within manX at 527.397 kb on - strand, within manX at 527.397 kb on - strand, within manX at 527.724 kb on + strand at 527.724 kb on + strand at 527.724 kb on + strand at 527.724 kb on + strand at 527.724 kb on + strand at 527.725 kb on - strand at 527.725 kb on - strand at 527.725 kb on - strand at 527.772 kb on + strand, within rapZ at 527.772 kb on + strand, within rapZ at 527.772 kb on + strand, within rapZ at 527.772 kb on + strand, within rapZ at 527.772 kb on + strand, within rapZ at 527.772 kb on + strand, within rapZ at 527.772 kb on + strand, within rapZ at 527.772 kb on + strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 528.033 kb on + strand, within rapZ at 528.169 kb on + strand, within rapZ at 528.170 kb on - strand, within rapZ
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Metal_ion_mix_sitestressplate 0.5 x remove 526,233 + ptsP OKGIIK_02235 0.67 +0.7 526,233 + ptsP OKGIIK_02235 0.67 -0.8 526,233 + ptsP OKGIIK_02235 0.67 +0.0 526,233 + ptsP OKGIIK_02235 0.67 -1.9 526,234 - ptsP OKGIIK_02235 0.67 +0.4 526,234 - ptsP OKGIIK_02235 0.67 -0.4 526,234 - ptsP OKGIIK_02235 0.67 -0.8 526,434 + ptsP OKGIIK_02235 0.79 +0.0 526,434 + ptsP OKGIIK_02235 0.79 -1.0 526,435 - ptsP OKGIIK_02235 0.79 -0.8 526,435 - ptsP OKGIIK_02235 0.79 -1.6 526,435 - ptsP OKGIIK_02235 0.79 +0.0 526,435 - ptsP OKGIIK_02235 0.79 +0.2 526,435 - ptsP OKGIIK_02235 0.79 -0.1 526,435 - ptsP OKGIIK_02235 0.79 -1.4 526,506 + ptsP OKGIIK_02235 0.83 -2.8 526,728 + -0.7 526,728 + +0.4 526,728 + -2.1 526,728 + -0.8 526,728 + -1.4 526,728 + -1.1 526,728 + +0.7 526,729 - -0.6 526,729 - -0.4 526,729 - +0.3 526,729 - -1.5 526,951 + -1.6 526,951 + +0.2 526,951 + -1.1 526,951 + -1.4 526,951 + -1.2 526,952 - -1.5 526,952 - -0.2 526,952 - -0.4 526,952 - +0.4 527,121 + OKGIIK_02240 0.63 +0.0 527,121 + OKGIIK_02240 0.63 +0.2 527,121 + OKGIIK_02240 0.63 +0.5 527,122 - OKGIIK_02240 0.64 -0.4 527,122 - OKGIIK_02240 0.64 -1.0 527,122 - OKGIIK_02240 0.64 -4.5 527,122 - OKGIIK_02240 0.64 -0.8 527,122 - OKGIIK_02240 0.64 -1.2 527,122 - OKGIIK_02240 0.64 +0.2 527,122 - OKGIIK_02240 0.64 -1.4 527,122 - OKGIIK_02240 0.64 -0.3 527,122 - OKGIIK_02240 0.64 -0.9 527,122 - OKGIIK_02240 0.64 -0.1 527,122 - OKGIIK_02240 0.64 -1.2 527,122 - OKGIIK_02240 0.64 -1.1 527,122 - OKGIIK_02240 0.64 -1.6 527,291 + manX OKGIIK_02245 0.20 +1.1 527,291 + manX OKGIIK_02245 0.20 -0.8 527,291 + manX OKGIIK_02245 0.20 -2.4 527,291 + manX OKGIIK_02245 0.20 +0.0 527,291 + manX OKGIIK_02245 0.20 -0.8 527,291 + manX OKGIIK_02245 0.20 -0.8 527,291 + manX OKGIIK_02245 0.20 -0.1 527,291 + manX OKGIIK_02245 0.20 -2.0 527,291 + manX OKGIIK_02245 0.20 -0.7 527,291 + manX OKGIIK_02245 0.20 -0.1 527,291 + manX OKGIIK_02245 0.20 -2.3 527,291 + manX OKGIIK_02245 0.20 +0.8 527,291 + manX OKGIIK_02245 0.20 -0.8 527,291 + manX OKGIIK_02245 0.20 -0.1 527,291 + manX OKGIIK_02245 0.20 -2.6 527,291 + manX OKGIIK_02245 0.20 +0.6 527,291 + manX OKGIIK_02245 0.20 +0.2 527,291 + manX OKGIIK_02245 0.20 -2.4 527,292 - manX OKGIIK_02245 0.20 -1.3 527,292 - manX OKGIIK_02245 0.20 -0.8 527,292 - manX OKGIIK_02245 0.20 -0.4 527,292 - manX OKGIIK_02245 0.20 -1.1 527,292 - manX OKGIIK_02245 0.20 +0.2 527,292 - manX OKGIIK_02245 0.20 -1.4 527,292 - manX OKGIIK_02245 0.20 -1.8 527,292 - manX OKGIIK_02245 0.20 -1.4 527,292 - manX OKGIIK_02245 0.20 -2.7 527,292 - manX OKGIIK_02245 0.20 +0.2 527,292 - manX OKGIIK_02245 0.20 -2.8 527,292 - manX OKGIIK_02245 0.20 -1.8 527,292 - manX OKGIIK_02245 0.20 +0.2 527,292 - manX OKGIIK_02245 0.20 -2.1 527,292 - manX OKGIIK_02245 0.20 -1.6 527,292 - manX OKGIIK_02245 0.20 +0.8 527,292 - manX OKGIIK_02245 0.20 -0.6 527,292 - manX OKGIIK_02245 0.20 -2.0 527,292 - manX OKGIIK_02245 0.20 +0.2 527,299 + manX OKGIIK_02245 0.22 -1.0 527,299 + manX OKGIIK_02245 0.22 -1.4 527,300 - manX OKGIIK_02245 0.22 -0.8 527,300 - manX OKGIIK_02245 0.22 -1.8 527,300 - manX OKGIIK_02245 0.22 -0.1 527,347 + manX OKGIIK_02245 0.34 -1.1 527,347 + manX OKGIIK_02245 0.34 -1.0 527,347 + manX OKGIIK_02245 0.34 -0.3 527,347 + manX OKGIIK_02245 0.34 -1.1 527,347 + manX OKGIIK_02245 0.34 -1.6 527,347 + manX OKGIIK_02245 0.34 -0.2 527,347 + manX OKGIIK_02245 0.34 -1.6 527,347 + manX OKGIIK_02245 0.34 -0.7 527,347 + manX OKGIIK_02245 0.34 -1.8 527,347 + manX OKGIIK_02245 0.34 +0.3 527,347 + manX OKGIIK_02245 0.34 -1.1 527,347 + manX OKGIIK_02245 0.34 -1.4 527,347 + manX OKGIIK_02245 0.34 -1.6 527,348 - manX OKGIIK_02245 0.35 +0.1 527,348 - manX OKGIIK_02245 0.35 -2.0 527,348 - manX OKGIIK_02245 0.35 -3.1 527,348 - manX OKGIIK_02245 0.35 -1.1 527,348 - manX OKGIIK_02245 0.35 +0.2 527,348 - manX OKGIIK_02245 0.35 +0.8 527,348 - manX OKGIIK_02245 0.35 -2.5 527,348 - manX OKGIIK_02245 0.35 +0.2 527,348 - manX OKGIIK_02245 0.35 -2.3 527,348 - manX OKGIIK_02245 0.35 -3.1 527,348 - manX OKGIIK_02245 0.35 -0.4 527,396 + manX OKGIIK_02245 0.47 -2.1 527,396 + manX OKGIIK_02245 0.47 -1.3 527,396 + manX OKGIIK_02245 0.47 +0.5 527,397 - manX OKGIIK_02245 0.47 -1.0 527,397 - manX OKGIIK_02245 0.47 +0.2 527,724 + -1.4 527,724 + +0.2 527,724 + -0.4 527,724 + -0.0 527,724 + -1.6 527,725 - -1.6 527,725 - +1.7 527,725 - -0.8 527,772 + rapZ OKGIIK_02250 0.14 -2.7 527,772 + rapZ OKGIIK_02250 0.14 -1.4 527,772 + rapZ OKGIIK_02250 0.14 -0.1 527,772 + rapZ OKGIIK_02250 0.14 +0.2 527,772 + rapZ OKGIIK_02250 0.14 -1.2 527,772 + rapZ OKGIIK_02250 0.14 -1.4 527,772 + rapZ OKGIIK_02250 0.14 -0.1 527,772 + rapZ OKGIIK_02250 0.14 -0.9 527,773 - rapZ OKGIIK_02250 0.14 +0.2 527,773 - rapZ OKGIIK_02250 0.14 -1.4 527,773 - rapZ OKGIIK_02250 0.14 +0.2 527,773 - rapZ OKGIIK_02250 0.14 -0.8 527,773 - rapZ OKGIIK_02250 0.14 -1.4 527,773 - rapZ OKGIIK_02250 0.14 -2.7 527,773 - rapZ OKGIIK_02250 0.14 -1.8 527,773 - rapZ OKGIIK_02250 0.14 -2.5 527,773 - rapZ OKGIIK_02250 0.14 -1.6 527,773 - rapZ OKGIIK_02250 0.14 -1.6 527,773 - rapZ OKGIIK_02250 0.14 -1.1 527,773 - rapZ OKGIIK_02250 0.14 -3.1 527,773 - rapZ OKGIIK_02250 0.14 -0.8 527,773 - rapZ OKGIIK_02250 0.14 -0.6 527,773 - rapZ OKGIIK_02250 0.14 +0.2 527,838 + rapZ OKGIIK_02250 0.21 -1.1 527,838 + rapZ OKGIIK_02250 0.21 -1.6 527,838 + rapZ OKGIIK_02250 0.21 -0.6 527,838 + rapZ OKGIIK_02250 0.21 -1.1 527,838 + rapZ OKGIIK_02250 0.21 -0.8 527,838 + rapZ OKGIIK_02250 0.21 -0.8 527,838 + rapZ OKGIIK_02250 0.21 -1.0 527,838 + rapZ OKGIIK_02250 0.21 -1.1 527,838 + rapZ OKGIIK_02250 0.21 -2.1 527,838 + rapZ OKGIIK_02250 0.21 -1.4 527,838 + rapZ OKGIIK_02250 0.21 -0.6 527,838 + rapZ OKGIIK_02250 0.21 -1.6 527,838 + rapZ OKGIIK_02250 0.21 -2.9 527,838 + rapZ OKGIIK_02250 0.21 -2.3 527,838 + rapZ OKGIIK_02250 0.21 -0.8 527,838 + rapZ OKGIIK_02250 0.21 -1.6 527,838 + rapZ OKGIIK_02250 0.21 -2.5 527,838 + rapZ OKGIIK_02250 0.21 -1.6 527,838 + rapZ OKGIIK_02250 0.21 -0.8 527,839 - rapZ OKGIIK_02250 0.21 -0.8 527,839 - rapZ OKGIIK_02250 0.21 -1.1 527,839 - rapZ OKGIIK_02250 0.21 -0.8 527,839 - rapZ OKGIIK_02250 0.21 -1.6 527,839 - rapZ OKGIIK_02250 0.21 -0.8 527,839 - rapZ OKGIIK_02250 0.21 -0.8 527,839 - rapZ OKGIIK_02250 0.21 -2.0 527,839 - rapZ OKGIIK_02250 0.21 -0.2 527,839 - rapZ OKGIIK_02250 0.21 -1.4 527,839 - rapZ OKGIIK_02250 0.21 -0.8 527,839 - rapZ OKGIIK_02250 0.21 -1.4 527,839 - rapZ OKGIIK_02250 0.21 -1.9 527,839 - rapZ OKGIIK_02250 0.21 -1.1 527,839 - rapZ OKGIIK_02250 0.21 -1.4 527,839 - rapZ OKGIIK_02250 0.21 -2.3 527,839 - rapZ OKGIIK_02250 0.21 -1.8 527,839 - rapZ OKGIIK_02250 0.21 -0.1 527,839 - rapZ OKGIIK_02250 0.21 -1.8 527,839 - rapZ OKGIIK_02250 0.21 -1.1 527,839 - rapZ OKGIIK_02250 0.21 -2.1 528,033 + rapZ OKGIIK_02250 0.43 -1.8 528,169 + rapZ OKGIIK_02250 0.58 +0.0 528,170 - rapZ OKGIIK_02250 0.58 -0.1
Or see this region's nucleotide sequence