Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF5622

Experiment: Ying_Nucleobase14 rep A; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5621 and GFF5622 are separated by 31 nucleotidesGFF5622 and GFF5623 are separated by 51 nucleotidesGFF5623 and GFF5624 overlap by 20 nucleotides GFF5621 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 2,640,030 to 2,641,238 GFF5621 GFF5622 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,641,270 to 2,642,061 GFF5622 GFF5623 - hypothetical protein, at 2,642,113 to 2,642,847 GFF5623 GFF5624 - L-lactate dehydrogenase (EC 1.1.2.3), at 2,642,828 to 2,644,087 GFF5624 Position (kb) 2641 2642 2643Strain fitness (log2 ratio) -1 0 1at 2640.686 kb on - strand, within GFF5621at 2640.734 kb on - strand, within GFF5621at 2640.734 kb on - strand, within GFF5621at 2640.757 kb on + strand, within GFF5621at 2641.239 kb on - strandat 2641.239 kb on - strandat 2641.559 kb on + strand, within GFF5622at 2641.560 kb on - strand, within GFF5622at 2642.114 kb on + strandat 2642.747 kb on + strand, within GFF5623at 2642.747 kb on + strand, within GFF5623at 2643.012 kb on + strand, within GFF5624

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 2
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2,640,686 - GFF5621 0.54 -0.7
2,640,734 - GFF5621 0.58 -0.2
2,640,734 - GFF5621 0.58 +1.0
2,640,757 + GFF5621 0.60 -0.5
2,641,239 - +0.2
2,641,239 - +0.1
2,641,559 + GFF5622 0.36 -0.7
2,641,560 - GFF5622 0.37 -0.7
2,642,114 + -0.5
2,642,747 + GFF5623 0.86 +0.6
2,642,747 + GFF5623 0.86 -0.6
2,643,012 + GFF5624 0.15 +0.1

Or see this region's nucleotide sequence