Experiment: Ying_Nucleobase14 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4864 and GFF4865 are separated by 25 nucleotides GFF4865 and GFF4866 overlap by 4 nucleotides GFF4866 and GFF4867 are separated by 7 nucleotides GFF4867 and GFF4868 are separated by 116 nucleotides
GFF4864 - FIG021952: putative membrane protein, at 1,871,285 to 1,872,163
GFF4864
GFF4865 - Type IV pilus biogenesis protein PilF, at 1,872,189 to 1,873,055
GFF4865
GFF4866 - Ribosomal RNA large subunit methyltransferase N (EC 2.1.1.-), at 1,873,052 to 1,874,200
GFF4866
GFF4867 - Nucleoside diphosphate kinase (EC 2.7.4.6), at 1,874,208 to 1,874,633
GFF4867
GFF4868 - Ribosomal large subunit pseudouridine synthase B (EC 4.2.1.70), at 1,874,750 to 1,875,961
GFF4868
Position (kb)
1873
1874
1875 Strain fitness (log2 ratio)
-1
0
1
2 at 1872.214 kb on + strand at 1872.215 kb on - strand at 1872.215 kb on - strand at 1872.215 kb on - strand at 1872.422 kb on - strand, within GFF4865 at 1872.422 kb on - strand, within GFF4865 at 1872.422 kb on - strand, within GFF4865 at 1872.422 kb on - strand, within GFF4865 at 1872.455 kb on - strand, within GFF4865 at 1872.455 kb on - strand, within GFF4865 at 1872.548 kb on - strand, within GFF4865 at 1872.737 kb on - strand, within GFF4865 at 1872.749 kb on - strand, within GFF4865 at 1873.307 kb on - strand, within GFF4866 at 1873.414 kb on - strand, within GFF4866 at 1873.414 kb on - strand, within GFF4866 at 1873.414 kb on - strand, within GFF4866 at 1873.489 kb on - strand, within GFF4866 at 1873.689 kb on + strand, within GFF4866 at 1873.690 kb on - strand, within GFF4866 at 1873.690 kb on - strand, within GFF4866 at 1873.690 kb on - strand, within GFF4866 at 1873.690 kb on - strand, within GFF4866 at 1873.690 kb on - strand, within GFF4866 at 1874.200 kb on + strand at 1874.200 kb on + strand at 1874.200 kb on + strand at 1874.200 kb on + strand at 1874.201 kb on - strand at 1874.201 kb on - strand at 1874.201 kb on - strand at 1874.218 kb on + strand at 1874.218 kb on + strand at 1874.219 kb on - strand at 1874.684 kb on - strand at 1874.700 kb on + strand at 1874.700 kb on + strand at 1874.700 kb on + strand at 1874.700 kb on + strand at 1874.700 kb on + strand at 1874.701 kb on - strand at 1874.701 kb on - strand at 1874.701 kb on - strand at 1874.856 kb on + strand at 1874.856 kb on + strand at 1874.856 kb on + strand at 1874.857 kb on - strand at 1874.857 kb on - strand at 1874.857 kb on - strand at 1874.857 kb on - strand at 1874.967 kb on + strand, within GFF4868 at 1874.968 kb on - strand, within GFF4868 at 1875.119 kb on + strand, within GFF4868 at 1875.120 kb on - strand, within GFF4868 at 1875.120 kb on - strand, within GFF4868
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 2 remove 1,872,214 + +0.3 1,872,215 - -0.9 1,872,215 - +0.2 1,872,215 - +0.0 1,872,422 - GFF4865 0.27 +0.6 1,872,422 - GFF4865 0.27 +0.5 1,872,422 - GFF4865 0.27 +0.1 1,872,422 - GFF4865 0.27 +1.1 1,872,455 - GFF4865 0.31 +0.2 1,872,455 - GFF4865 0.31 +0.4 1,872,548 - GFF4865 0.41 +0.9 1,872,737 - GFF4865 0.63 +0.7 1,872,749 - GFF4865 0.65 +1.3 1,873,307 - GFF4866 0.22 +1.5 1,873,414 - GFF4866 0.32 +1.5 1,873,414 - GFF4866 0.32 +0.8 1,873,414 - GFF4866 0.32 +1.1 1,873,489 - GFF4866 0.38 -0.4 1,873,689 + GFF4866 0.55 +0.5 1,873,690 - GFF4866 0.56 +0.0 1,873,690 - GFF4866 0.56 +1.2 1,873,690 - GFF4866 0.56 +0.4 1,873,690 - GFF4866 0.56 +0.4 1,873,690 - GFF4866 0.56 +0.2 1,874,200 + +0.5 1,874,200 + +2.1 1,874,200 + +0.2 1,874,200 + +1.5 1,874,201 - +0.5 1,874,201 - -0.5 1,874,201 - +0.6 1,874,218 + +0.3 1,874,218 + -0.9 1,874,219 - -0.1 1,874,684 - -1.0 1,874,700 + -0.0 1,874,700 + +0.6 1,874,700 + -0.5 1,874,700 + -0.1 1,874,700 + -0.1 1,874,701 - +0.1 1,874,701 - -0.6 1,874,701 - -0.1 1,874,856 + +0.2 1,874,856 + -0.9 1,874,856 + -0.6 1,874,857 - +0.4 1,874,857 - -0.3 1,874,857 - -0.0 1,874,857 - +0.2 1,874,967 + GFF4868 0.18 +0.3 1,874,968 - GFF4868 0.18 -0.2 1,875,119 + GFF4868 0.30 +0.5 1,875,120 - GFF4868 0.31 +0.2 1,875,120 - GFF4868 0.31 -0.3
Or see this region's nucleotide sequence