Experiment: Ying_Nucleobase14 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4115 and GFF4116 overlap by 4 nucleotides GFF4116 and GFF4117 are separated by 10 nucleotides GFF4117 and GFF4118 are separated by 34 nucleotides GFF4118 and GFF4119 overlap by 4 nucleotides GFF4119 and GFF4120 are separated by 16 nucleotides
GFF4115 - Flp pilus assembly protein TadG, at 1,027,951 to 1,028,415
GFF4115
GFF4116 - Flp pilus assembly protein TadG, at 1,028,412 to 1,028,906
GFF4116
GFF4117 - hypothetical protein, at 1,028,917 to 1,029,207
GFF4117
GFF4118 - Flp pilus assembly protein TadD, contains TPR repeats, at 1,029,242 to 1,030,024
GFF4118
GFF4119 - Type II/IV secretion system protein TadC, associated with Flp pilus assembly, at 1,030,021 to 1,030,950
GFF4119
GFF4120 - Flp pilus assembly protein TadB, at 1,030,967 to 1,031,911
GFF4120
Position (kb)
1029
1030
1031 Strain fitness (log2 ratio)
-2
-1
0
1 at 1028.312 kb on + strand, within GFF4115 at 1028.312 kb on + strand, within GFF4115 at 1028.312 kb on + strand, within GFF4115 at 1028.806 kb on + strand, within GFF4116 at 1028.807 kb on - strand, within GFF4116 at 1028.854 kb on + strand, within GFF4116 at 1028.918 kb on + strand at 1028.918 kb on + strand at 1028.919 kb on - strand at 1028.981 kb on + strand, within GFF4117 at 1028.981 kb on + strand, within GFF4117 at 1029.441 kb on + strand, within GFF4118 at 1029.441 kb on + strand, within GFF4118 at 1029.442 kb on - strand, within GFF4118 at 1029.624 kb on + strand, within GFF4118 at 1029.624 kb on + strand, within GFF4118 at 1029.702 kb on + strand, within GFF4118 at 1029.703 kb on - strand, within GFF4118 at 1029.703 kb on - strand, within GFF4118 at 1029.864 kb on + strand, within GFF4118 at 1029.864 kb on + strand, within GFF4118 at 1029.865 kb on - strand, within GFF4118 at 1030.305 kb on - strand, within GFF4119 at 1030.305 kb on - strand, within GFF4119 at 1030.305 kb on - strand, within GFF4119 at 1030.872 kb on - strand at 1030.872 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 2 remove 1,028,312 + GFF4115 0.78 +0.5 1,028,312 + GFF4115 0.78 -1.2 1,028,312 + GFF4115 0.78 -1.9 1,028,806 + GFF4116 0.80 -0.8 1,028,807 - GFF4116 0.80 -0.8 1,028,854 + GFF4116 0.89 +0.0 1,028,918 + -0.5 1,028,918 + -0.6 1,028,919 - -0.8 1,028,981 + GFF4117 0.22 -0.0 1,028,981 + GFF4117 0.22 -1.0 1,029,441 + GFF4118 0.25 -0.1 1,029,441 + GFF4118 0.25 -0.6 1,029,442 - GFF4118 0.26 -0.7 1,029,624 + GFF4118 0.49 -0.5 1,029,624 + GFF4118 0.49 -0.7 1,029,702 + GFF4118 0.59 -0.2 1,029,703 - GFF4118 0.59 -0.8 1,029,703 - GFF4118 0.59 +0.2 1,029,864 + GFF4118 0.79 +0.2 1,029,864 + GFF4118 0.79 +0.5 1,029,865 - GFF4118 0.80 +0.0 1,030,305 - GFF4119 0.31 +0.5 1,030,305 - GFF4119 0.31 -0.7 1,030,305 - GFF4119 0.31 -0.1 1,030,872 - -1.7 1,030,872 - -1.1
Or see this region's nucleotide sequence