Experiment: Ying_Nucleobase14 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4063 and GFF4064 are separated by 24 nucleotides GFF4064 and GFF4065 overlap by 4 nucleotides GFF4065 and GFF4066 are separated by 22 nucleotides GFF4066 and GFF4067 overlap by 14 nucleotides
GFF4063 - Flagellar hook-length control protein FliK, at 980,245 to 981,282
GFF4063
GFF4064 - hypothetical protein, at 981,307 to 981,759
GFF4064
GFF4065 - RNA polymerase sigma factor for flagellar operon, at 981,756 to 982,481
GFF4065
GFF4066 - Flagellar motor rotation protein MotA, at 982,504 to 983,424
GFF4066
GFF4067 - Flagellar motor rotation protein MotB, at 983,411 to 984,433
GFF4067
Position (kb) 981
982
983 Strain fitness (log2 ratio) -1
0
1 at 981.383 kb on - strand, within GFF4064 at 981.451 kb on + strand, within GFF4064 at 981.514 kb on + strand, within GFF4064 at 981.514 kb on + strand, within GFF4064 at 981.515 kb on - strand, within GFF4064 at 981.682 kb on + strand, within GFF4064 at 981.772 kb on - strand at 981.772 kb on - strand at 982.180 kb on - strand, within GFF4065 at 982.306 kb on + strand, within GFF4065 at 982.306 kb on + strand, within GFF4065 at 982.307 kb on - strand, within GFF4065 at 982.359 kb on + strand, within GFF4065 at 982.368 kb on + strand, within GFF4065 at 982.750 kb on + strand, within GFF4066 at 982.750 kb on + strand, within GFF4066 at 982.750 kb on + strand, within GFF4066 at 982.838 kb on - strand, within GFF4066 at 983.156 kb on - strand, within GFF4066
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 2 remove 981,383 - GFF4064 0.17 +0.5 981,451 + GFF4064 0.32 +0.1 981,514 + GFF4064 0.46 +1.2 981,514 + GFF4064 0.46 +0.5 981,515 - GFF4064 0.46 +0.0 981,682 + GFF4064 0.83 +0.4 981,772 - -0.8 981,772 - +0.5 982,180 - GFF4065 0.58 +0.5 982,306 + GFF4065 0.76 -0.7 982,306 + GFF4065 0.76 +0.4 982,307 - GFF4065 0.76 +0.8 982,359 + GFF4065 0.83 +0.3 982,368 + GFF4065 0.84 +1.1 982,750 + GFF4066 0.27 -1.7 982,750 + GFF4066 0.27 -0.3 982,750 + GFF4066 0.27 -0.4 982,838 - GFF4066 0.36 -1.3 983,156 - GFF4066 0.71 -0.7
Or see this region's nucleotide sequence