Experiment: Ying_Nucleobase14 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3688 and GFF3689 are separated by 15 nucleotides GFF3689 and GFF3690 are separated by 52 nucleotides GFF3690 and GFF3691 overlap by 4 nucleotides GFF3691 and GFF3692 are separated by 40 nucleotides
GFF3688 - NADH-ubiquinone oxidoreductase chain H (EC 1.6.5.3), at 578,371 to 579,447
GFF3688
GFF3689 - NADH-ubiquinone oxidoreductase chain I (EC 1.6.5.3), at 579,463 to 579,999
GFF3689
GFF3690 - NADH-ubiquinone oxidoreductase chain J (EC 1.6.5.3), at 580,052 to 580,702
GFF3690
GFF3691 - NADH-ubiquinone oxidoreductase chain K (EC 1.6.5.3), at 580,699 to 581,007
GFF3691
GFF3692 - NADH-ubiquinone oxidoreductase chain L (EC 1.6.5.3), at 581,048 to 583,099
GFF3692
Position (kb) 580
581 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 579.340 kb on + strand at 579.445 kb on + strand at 579.445 kb on + strand at 579.445 kb on + strand at 579.949 kb on + strand at 579.950 kb on - strand at 580.101 kb on - strand at 580.101 kb on - strand at 580.130 kb on - strand, within GFF3690 at 580.476 kb on - strand, within GFF3690 at 580.490 kb on + strand, within GFF3690 at 581.432 kb on + strand, within GFF3692 at 581.433 kb on - strand, within GFF3692 at 581.666 kb on + strand, within GFF3692
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 2 remove 579,340 + -0.8 579,445 + +0.2 579,445 + +0.3 579,445 + -0.5 579,949 + +0.1 579,950 - -2.8 580,101 - -2.9 580,101 - -2.2 580,130 - GFF3690 0.12 -1.4 580,476 - GFF3690 0.65 -2.5 580,490 + GFF3690 0.67 -1.5 581,432 + GFF3692 0.19 -0.9 581,433 - GFF3692 0.19 -0.7 581,666 + GFF3692 0.30 +0.2
Or see this region's nucleotide sequence