Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF3400

Experiment: Ying_Nucleobase14 rep A; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3399 and GFF3400 overlap by 1 nucleotidesGFF3400 and GFF3401 are separated by 4 nucleotides GFF3399 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 283,805 to 284,854 GFF3399 GFF3400 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 284,854 to 285,738 GFF3400 GFF3401 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 285,743 to 287,683 GFF3401 Position (kb) 284 285 286Strain fitness (log2 ratio) -1 0 1at 284.161 kb on - strand, within GFF3399at 284.331 kb on + strand, within GFF3399at 284.332 kb on - strand, within GFF3399at 284.478 kb on + strand, within GFF3399at 284.601 kb on + strand, within GFF3399at 284.830 kb on + strandat 284.972 kb on + strand, within GFF3400at 284.972 kb on + strand, within GFF3400at 284.973 kb on - strand, within GFF3400at 285.654 kb on - strandat 285.719 kb on + strandat 285.823 kb on - strandat 285.864 kb on + strandat 286.194 kb on + strand, within GFF3401at 286.194 kb on + strand, within GFF3401at 286.195 kb on - strand, within GFF3401at 286.285 kb on - strand, within GFF3401

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 2
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284,161 - GFF3399 0.34 +0.2
284,331 + GFF3399 0.50 -0.0
284,332 - GFF3399 0.50 -0.1
284,478 + GFF3399 0.64 +0.1
284,601 + GFF3399 0.76 -0.3
284,830 + -0.2
284,972 + GFF3400 0.13 -0.8
284,972 + GFF3400 0.13 +0.4
284,973 - GFF3400 0.13 +1.2
285,654 - -0.1
285,719 + +0.2
285,823 - -0.9
285,864 + +0.2
286,194 + GFF3401 0.23 +0.3
286,194 + GFF3401 0.23 -0.2
286,195 - GFF3401 0.23 +0.5
286,285 - GFF3401 0.28 -0.5

Or see this region's nucleotide sequence