Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF3373

Experiment: Ying_Nucleobase14 rep A; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3372 and GFF3373 are separated by 18 nucleotidesGFF3373 and GFF3374 are separated by 65 nucleotides GFF3372 - Malate dehydrogenase (EC 1.1.1.37), at 256,455 to 257,441 GFF3372 GFF3373 - Citrate lyase beta chain (EC 4.1.3.6), at 257,460 to 258,464 GFF3373 GFF3374 - Aconitate hydratase 2 (EC 4.2.1.3) @ 2-methylisocitrate dehydratase (EC 4.2.1.99), at 258,530 to 261,130 GFF3374 Position (kb) 257 258 259Strain fitness (log2 ratio) -3 -2 -1 0 1at 257.535 kb on + strandat 257.536 kb on - strandat 258.131 kb on - strand, within GFF3373at 258.196 kb on - strand, within GFF3373at 258.196 kb on - strand, within GFF3373at 258.360 kb on + strand, within GFF3373at 258.361 kb on - strand, within GFF3373at 258.366 kb on + strandat 258.366 kb on + strandat 258.367 kb on - strandat 258.475 kb on - strandat 258.828 kb on - strand, within GFF3374at 259.248 kb on - strand, within GFF3374at 259.248 kb on - strand, within GFF3374

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 2
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257,535 + -0.3
257,536 - +0.0
258,131 - GFF3373 0.67 -0.9
258,196 - GFF3373 0.73 +0.3
258,196 - GFF3373 0.73 +0.1
258,360 + GFF3373 0.90 -0.9
258,361 - GFF3373 0.90 -2.8
258,366 + +0.5
258,366 + -0.0
258,367 - -0.4
258,475 - -0.2
258,828 - GFF3374 0.11 +0.0
259,248 - GFF3374 0.28 -0.5
259,248 - GFF3374 0.28 -1.2

Or see this region's nucleotide sequence