Experiment: Ying_Nucleobase14 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3355 and GFF3356 are separated by 43 nucleotides GFF3356 and GFF3357 overlap by 4 nucleotides GFF3357 and GFF3358 are separated by 71 nucleotides
GFF3355 - Tryptophan synthase beta chain (EC 4.2.1.20), at 238,822 to 240,093
GFF3355
GFF3356 - Phosphoribosylanthranilate isomerase (EC 5.3.1.24), at 240,137 to 240,817
GFF3356
GFF3357 - tRNA pseudouridine synthase A (EC 4.2.1.70), at 240,814 to 241,581
GFF3357
GFF3358 - Translation initiation factor 2, at 241,653 to 244,334
GFF3358
Position (kb) 240
241 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 239.327 kb on + strand, within GFF3355 at 239.519 kb on + strand, within GFF3355 at 239.519 kb on + strand, within GFF3355 at 239.520 kb on - strand, within GFF3355 at 239.637 kb on - strand, within GFF3355 at 240.044 kb on + strand at 240.044 kb on + strand at 240.080 kb on + strand at 240.081 kb on - strand at 240.335 kb on + strand, within GFF3356 at 240.335 kb on + strand, within GFF3356 at 240.335 kb on + strand, within GFF3356 at 240.335 kb on + strand, within GFF3356 at 240.393 kb on + strand, within GFF3356 at 240.393 kb on + strand, within GFF3356 at 240.394 kb on - strand, within GFF3356 at 240.451 kb on - strand, within GFF3356 at 240.456 kb on - strand, within GFF3356 at 240.712 kb on - strand, within GFF3356 at 240.712 kb on - strand, within GFF3356 at 240.712 kb on - strand, within GFF3356 at 240.869 kb on + strand at 240.869 kb on + strand at 240.869 kb on + strand at 240.869 kb on + strand at 240.869 kb on + strand at 240.887 kb on + strand at 241.058 kb on + strand, within GFF3357 at 241.059 kb on - strand, within GFF3357 at 241.059 kb on - strand, within GFF3357 at 241.059 kb on - strand, within GFF3357 at 241.265 kb on + strand, within GFF3357 at 241.266 kb on - strand, within GFF3357 at 241.266 kb on - strand, within GFF3357 at 241.266 kb on - strand, within GFF3357 at 241.266 kb on - strand, within GFF3357 at 241.559 kb on + strand at 241.574 kb on + strand at 241.574 kb on + strand at 241.575 kb on - strand at 241.575 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 2 remove 239,327 + GFF3355 0.40 -1.5 239,519 + GFF3355 0.55 -2.3 239,519 + GFF3355 0.55 -2.1 239,520 - GFF3355 0.55 -1.4 239,637 - GFF3355 0.64 -2.2 240,044 + -0.5 240,044 + -1.0 240,080 + -1.1 240,081 - -2.3 240,335 + GFF3356 0.29 -1.5 240,335 + GFF3356 0.29 -2.7 240,335 + GFF3356 0.29 -1.9 240,335 + GFF3356 0.29 -3.5 240,393 + GFF3356 0.38 -0.9 240,393 + GFF3356 0.38 -2.0 240,394 - GFF3356 0.38 -2.5 240,451 - GFF3356 0.46 -2.0 240,456 - GFF3356 0.47 -1.1 240,712 - GFF3356 0.84 -0.9 240,712 - GFF3356 0.84 -1.1 240,712 - GFF3356 0.84 -2.0 240,869 + +1.3 240,869 + -0.0 240,869 + +0.6 240,869 + +1.3 240,869 + +0.6 240,887 + +2.4 241,058 + GFF3357 0.32 +0.6 241,059 - GFF3357 0.32 +2.0 241,059 - GFF3357 0.32 -2.4 241,059 - GFF3357 0.32 +0.0 241,265 + GFF3357 0.59 -0.6 241,266 - GFF3357 0.59 -0.6 241,266 - GFF3357 0.59 +0.5 241,266 - GFF3357 0.59 -0.6 241,266 - GFF3357 0.59 +1.2 241,559 + -0.3 241,574 + +1.0 241,574 + -0.7 241,575 - +0.3 241,575 - +0.0
Or see this region's nucleotide sequence