Experiment: Ying_Nucleobase14 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3222 and GFF3223 are separated by 46 nucleotides GFF3223 and GFF3224 overlap by 4 nucleotides
GFF3222 - Probable transmembrane protein, at 103,459 to 104,223
GFF3222
GFF3223 - Piwi domain protein, at 104,270 to 105,724
GFF3223
GFF3224 - hypothetical protein, at 105,721 to 107,712
GFF3224
Position (kb) 104
105
106 Strain fitness (log2 ratio) -1
0
1 at 103.299 kb on - strand at 103.313 kb on - strand at 103.357 kb on - strand at 103.418 kb on + strand at 103.480 kb on - strand at 103.480 kb on - strand at 103.582 kb on + strand, within GFF3222 at 103.582 kb on + strand, within GFF3222 at 103.582 kb on + strand, within GFF3222 at 103.583 kb on - strand, within GFF3222 at 103.583 kb on - strand, within GFF3222 at 103.583 kb on - strand, within GFF3222 at 103.583 kb on - strand, within GFF3222 at 103.583 kb on - strand, within GFF3222 at 103.583 kb on - strand, within GFF3222 at 103.583 kb on - strand, within GFF3222 at 103.750 kb on + strand, within GFF3222 at 103.750 kb on + strand, within GFF3222 at 103.750 kb on + strand, within GFF3222 at 103.751 kb on - strand, within GFF3222 at 103.751 kb on - strand, within GFF3222 at 103.751 kb on - strand, within GFF3222 at 103.932 kb on + strand, within GFF3222 at 103.933 kb on - strand, within GFF3222 at 103.933 kb on - strand, within GFF3222 at 103.981 kb on + strand, within GFF3222 at 103.982 kb on - strand, within GFF3222 at 104.272 kb on - strand at 104.272 kb on - strand at 104.506 kb on - strand, within GFF3223 at 104.506 kb on - strand, within GFF3223 at 104.808 kb on + strand, within GFF3223 at 104.970 kb on + strand, within GFF3223 at 104.970 kb on + strand, within GFF3223 at 105.004 kb on - strand, within GFF3223 at 105.004 kb on - strand, within GFF3223 at 105.085 kb on - strand, within GFF3223 at 105.453 kb on + strand, within GFF3223 at 105.540 kb on + strand, within GFF3223 at 105.540 kb on + strand, within GFF3223 at 105.564 kb on + strand, within GFF3223 at 105.574 kb on - strand, within GFF3223 at 105.914 kb on + strand at 105.914 kb on + strand at 105.914 kb on + strand at 105.914 kb on + strand at 106.134 kb on - strand, within GFF3224 at 106.200 kb on + strand, within GFF3224 at 106.200 kb on + strand, within GFF3224 at 106.201 kb on - strand, within GFF3224 at 106.290 kb on - strand, within GFF3224 at 106.290 kb on - strand, within GFF3224 at 106.408 kb on + strand, within GFF3224 at 106.408 kb on + strand, within GFF3224 at 106.409 kb on - strand, within GFF3224
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 2 remove 103,299 - +0.2 103,313 - -0.6 103,357 - +0.3 103,418 + +0.5 103,480 - -0.9 103,480 - +0.7 103,582 + GFF3222 0.16 +1.6 103,582 + GFF3222 0.16 +0.2 103,582 + GFF3222 0.16 -1.3 103,583 - GFF3222 0.16 +0.6 103,583 - GFF3222 0.16 +0.1 103,583 - GFF3222 0.16 -0.4 103,583 - GFF3222 0.16 -0.9 103,583 - GFF3222 0.16 -1.3 103,583 - GFF3222 0.16 -0.8 103,583 - GFF3222 0.16 -1.6 103,750 + GFF3222 0.38 -0.2 103,750 + GFF3222 0.38 -0.6 103,750 + GFF3222 0.38 +0.0 103,751 - GFF3222 0.38 +0.4 103,751 - GFF3222 0.38 -0.1 103,751 - GFF3222 0.38 +0.3 103,932 + GFF3222 0.62 -1.3 103,933 - GFF3222 0.62 +0.5 103,933 - GFF3222 0.62 +0.1 103,981 + GFF3222 0.68 +0.2 103,982 - GFF3222 0.68 -0.1 104,272 - +0.3 104,272 - -0.4 104,506 - GFF3223 0.16 -0.5 104,506 - GFF3223 0.16 -1.7 104,808 + GFF3223 0.37 +0.4 104,970 + GFF3223 0.48 -0.6 104,970 + GFF3223 0.48 -1.4 105,004 - GFF3223 0.50 -0.3 105,004 - GFF3223 0.50 -0.8 105,085 - GFF3223 0.56 -0.2 105,453 + GFF3223 0.81 +0.0 105,540 + GFF3223 0.87 +0.4 105,540 + GFF3223 0.87 +0.0 105,564 + GFF3223 0.89 -0.3 105,574 - GFF3223 0.90 -1.1 105,914 + -0.7 105,914 + -0.4 105,914 + +0.2 105,914 + -0.3 106,134 - GFF3224 0.21 -1.0 106,200 + GFF3224 0.24 +0.1 106,200 + GFF3224 0.24 +0.2 106,201 - GFF3224 0.24 +0.4 106,290 - GFF3224 0.29 +0.2 106,290 - GFF3224 0.29 -0.6 106,408 + GFF3224 0.34 -0.4 106,408 + GFF3224 0.34 -0.1 106,409 - GFF3224 0.35 -0.2
Or see this region's nucleotide sequence