Experiment: Ying_Nucleobase14 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3148 and GFF3149 overlap by 14 nucleotides GFF3149 and GFF3150 are separated by 14 nucleotides GFF3150 and GFF3151 are separated by 30 nucleotides GFF3151 and GFF3152 are separated by 157 nucleotides GFF3152 and GFF3153 are separated by 374 nucleotides
GFF3148 - IncF plasmid conjugative transfer pilus assembly protein TraK, at 26,888 to 27,883
GFF3148
GFF3149 - IncF plasmid conjugative transfer pilus assembly protein TraE, at 27,870 to 28,436
GFF3149
GFF3150 - IncF plasmid conjugative transfer pilus assembly protein TraL, at 28,451 to 28,750
GFF3150
GFF3151 - hypothetical protein, at 28,781 to 29,143
GFF3151
GFF3152 - FIG003465: Conjugative transfer outer membrane protein, at 29,301 to 29,651
GFF3152
GFF3153 - Conjugative transfer transglycosylase, at 30,026 to 30,559
GFF3153
Position (kb) 28
29
30 Strain fitness (log2 ratio) -2
-1
0
1 at 28.142 kb on - strand, within GFF3149 at 28.232 kb on - strand, within GFF3149 at 28.734 kb on + strand at 28.734 kb on + strand at 28.734 kb on + strand at 28.735 kb on - strand at 28.851 kb on + strand, within GFF3151 at 28.851 kb on + strand, within GFF3151 at 28.852 kb on - strand, within GFF3151 at 30.132 kb on + strand, within GFF3153 at 30.133 kb on - strand, within GFF3153
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 2 remove 28,142 - GFF3149 0.48 +0.2 28,232 - GFF3149 0.64 -0.3 28,734 + -2.1 28,734 + +0.2 28,734 + +0.9 28,735 - -0.4 28,851 + GFF3151 0.19 +1.3 28,851 + GFF3151 0.19 +0.4 28,852 - GFF3151 0.20 +0.1 30,132 + GFF3153 0.20 -0.5 30,133 - GFF3153 0.20 -0.7
Or see this region's nucleotide sequence