Experiment: Ying_Nucleobase14 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2714 and GFF2715 are separated by 19 nucleotides GFF2715 and GFF2716 are separated by 80 nucleotides GFF2716 and GFF2717 are separated by 36 nucleotides
GFF2714 - D-amino acid dehydrogenase (EC 1.4.99.1) family protein in hydroxy-L-proline catabolic cluster, at 305,185 to 306,420
GFF2714
GFF2715 - rRNA small subunit methyltransferase I, at 306,440 to 307,282
GFF2715
GFF2716 - Predicted endonuclease distantly related to archaeal Holliday junction resolvase, at 307,363 to 307,767
GFF2716
GFF2717 - Phosphoheptose isomerase (EC 5.3.1.-), at 307,804 to 308,427
GFF2717
Position (kb) 306
307
308 Strain fitness (log2 ratio) -2
-1
0
1 at 305.647 kb on + strand, within GFF2714 at 305.647 kb on + strand, within GFF2714 at 305.989 kb on + strand, within GFF2714 at 307.062 kb on + strand, within GFF2715 at 307.062 kb on + strand, within GFF2715 at 307.062 kb on + strand, within GFF2715 at 307.063 kb on - strand, within GFF2715 at 307.284 kb on + strand at 307.284 kb on + strand at 307.284 kb on + strand at 307.285 kb on - strand at 307.285 kb on - strand at 307.285 kb on - strand at 307.285 kb on - strand at 307.285 kb on - strand at 307.285 kb on - strand at 307.285 kb on - strand at 307.777 kb on + strand at 307.777 kb on + strand at 307.777 kb on + strand at 307.777 kb on + strand at 307.777 kb on + strand at 307.778 kb on - strand at 307.793 kb on - strand at 307.855 kb on + strand at 307.855 kb on + strand at 307.855 kb on + strand at 307.856 kb on - strand at 307.856 kb on - strand at 307.856 kb on - strand at 307.856 kb on - strand at 307.856 kb on - strand at 307.856 kb on - strand at 307.856 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 2 remove 305,647 + GFF2714 0.37 -0.0 305,647 + GFF2714 0.37 -0.1 305,989 + GFF2714 0.65 -0.4 307,062 + GFF2715 0.74 -1.2 307,062 + GFF2715 0.74 -2.1 307,062 + GFF2715 0.74 -1.5 307,063 - GFF2715 0.74 -1.1 307,284 + +0.1 307,284 + -2.0 307,284 + -0.9 307,285 - -1.5 307,285 - -1.9 307,285 - -0.6 307,285 - +0.1 307,285 - -1.9 307,285 - -0.1 307,285 - -0.7 307,777 + -0.4 307,777 + -0.5 307,777 + -0.1 307,777 + -0.1 307,777 + -0.4 307,778 - +0.1 307,793 - -0.5 307,855 + -0.7 307,855 + -0.8 307,855 + -1.3 307,856 - -0.4 307,856 - -0.2 307,856 - -2.2 307,856 - +1.5 307,856 - -0.5 307,856 - -0.4 307,856 - -1.1
Or see this region's nucleotide sequence