Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF2201

Experiment: Ying_Nucleobase14 rep A; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2200 and GFF2201 overlap by 4 nucleotidesGFF2201 and GFF2202 are separated by 2 nucleotides GFF2200 - Transketolase (EC 2.2.1.1), at 1,089,172 to 1,091,247 GFF2200 GFF2201 - Phosphoglycolate phosphatase (EC 3.1.3.18), at 1,091,244 to 1,091,912 GFF2201 GFF2202 - NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12), at 1,091,915 to 1,092,778 GFF2202 Position (kb) 1091 1092Strain fitness (log2 ratio) -1 0 1at 1090.537 kb on + strand, within GFF2200at 1090.838 kb on - strand, within GFF2200at 1091.143 kb on + strandat 1091.143 kb on + strandat 1091.535 kb on + strand, within GFF2201at 1091.535 kb on + strand, within GFF2201at 1091.536 kb on - strand, within GFF2201at 1091.832 kb on + strand, within GFF2201at 1091.838 kb on + strand, within GFF2201

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 2
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1,090,537 + GFF2200 0.66 -0.0
1,090,838 - GFF2200 0.80 -0.7
1,091,143 + -0.9
1,091,143 + -1.1
1,091,535 + GFF2201 0.43 -1.3
1,091,535 + GFF2201 0.43 -1.5
1,091,536 - GFF2201 0.44 +0.1
1,091,832 + GFF2201 0.88 -0.7
1,091,838 + GFF2201 0.89 +0.0

Or see this region's nucleotide sequence