Experiment: Ying_Nucleobase14 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2096 and GFF2097 overlap by 4 nucleotides GFF2097 and GFF2098 overlap by 4 nucleotides GFF2098 and GFF2099 are separated by 82 nucleotides GFF2099 and GFF2100 overlap by 4 nucleotides
GFF2096 - D-2-hydroxyglutarate dehydrogenase, at 983,268 to 984,656
GFF2096
GFF2097 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 984,653 to 985,621
GFF2097
GFF2098 - Dihydroxy-acid dehydratase (EC 4.2.1.9), at 985,618 to 987,507
GFF2098
GFF2099 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 987,590 to 988,366
GFF2099
GFF2100 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 988,363 to 989,079
GFF2100
Position (kb) 985
986
987
988 Strain fitness (log2 ratio) -2
-1
0
1 at 985.901 kb on - strand, within GFF2098 at 985.943 kb on - strand, within GFF2098 at 986.587 kb on + strand, within GFF2098 at 986.588 kb on - strand, within GFF2098 at 986.588 kb on - strand, within GFF2098 at 986.872 kb on + strand, within GFF2098 at 987.392 kb on + strand at 987.393 kb on - strand at 987.413 kb on - strand at 987.506 kb on - strand at 987.559 kb on - strand at 988.031 kb on + strand, within GFF2099 at 988.347 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 2 remove 985,901 - GFF2098 0.15 -0.0 985,943 - GFF2098 0.17 -2.5 986,587 + GFF2098 0.51 -0.4 986,588 - GFF2098 0.51 +1.0 986,588 - GFF2098 0.51 -0.5 986,872 + GFF2098 0.66 +0.1 987,392 + -0.4 987,393 - -0.5 987,413 - -0.0 987,506 - -0.2 987,559 - -1.0 988,031 + GFF2099 0.57 +0.2 988,347 - +1.6
Or see this region's nucleotide sequence