Experiment: Ying_Nucleobase14 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1798 and GFF1799 overlap by 4 nucleotides GFF1799 and GFF2355 are separated by 161 nucleotides GFF2355 and GFF1800 are separated by 30 nucleotides GFF1800 and GFF1801 are separated by 11 nucleotides GFF1801 and GFF1802 overlap by 4 nucleotides GFF1802 and GFF1803 are separated by 152 nucleotides
GFF1798 - Transcription antitermination protein NusG, at 668,241 to 668,807
GFF1798
GFF1799 - Preprotein translocase subunit SecE (TC 3.A.5.1.1), at 668,804 to 669,187
GFF1799
GFF2355 - tRNA-Trp-CCA, at 669,349 to 669,421
GFF2355
GFF1800 - Translation elongation factor Tu, at 669,452 to 669,589
GFF1800
GFF1801 - Translation elongation factor Tu, at 669,601 to 670,755
GFF1801
GFF1802 - Translation elongation factor Tu, at 670,752 to 670,940
GFF1802
GFF1803 - Translation elongation factor G, at 671,093 to 673,195
GFF1803
Position (kb) 669
670
671 Strain fitness (log2 ratio) -2
-1
0
1 at 668.833 kb on - strand at 669.207 kb on - strand at 669.858 kb on - strand, within GFF1801 at 670.953 kb on - strand at 671.149 kb on - strand at 671.149 kb on - strand at 671.170 kb on - strand at 671.170 kb on - strand at 671.170 kb on - strand at 671.170 kb on - strand at 671.170 kb on - strand at 671.332 kb on - strand, within GFF1803 at 671.464 kb on - strand, within GFF1803 at 671.464 kb on - strand, within GFF1803 at 671.566 kb on - strand, within GFF1803
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 2 remove 668,833 - +1.2 669,207 - +1.2 669,858 - GFF1801 0.22 -1.0 670,953 - -1.1 671,149 - +1.7 671,149 - +1.3 671,170 - +1.1 671,170 - +1.2 671,170 - -0.3 671,170 - +0.4 671,170 - +0.4 671,332 - GFF1803 0.11 +0.7 671,464 - GFF1803 0.18 -1.3 671,464 - GFF1803 0.18 -1.2 671,566 - GFF1803 0.22 -2.4
Or see this region's nucleotide sequence