Experiment: Ying_Nucleobase14 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1660 and GFF1661 are separated by 106 nucleotides GFF1661 and GFF1662 overlap by 17 nucleotides
GFF1660 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 512,465 to 514,837
GFF1660
GFF1661 - Phosphoglycerate mutase (EC 5.4.2.1), at 514,944 to 515,585
GFF1661
GFF1662 - low-specificity D-threonine aldolase, at 515,569 to 516,753
GFF1662
Position (kb) 514
515
516 Strain fitness (log2 ratio) -2
-1
0
1 at 514.260 kb on + strand, within GFF1660 at 514.260 kb on + strand, within GFF1660 at 514.260 kb on + strand, within GFF1660 at 514.261 kb on - strand, within GFF1660 at 514.311 kb on + strand, within GFF1660 at 514.311 kb on + strand, within GFF1660 at 514.311 kb on + strand, within GFF1660 at 514.312 kb on - strand, within GFF1660 at 514.344 kb on + strand, within GFF1660 at 514.488 kb on + strand, within GFF1660 at 514.489 kb on - strand, within GFF1660 at 514.489 kb on - strand, within GFF1660 at 514.489 kb on - strand, within GFF1660 at 514.489 kb on - strand, within GFF1660 at 514.492 kb on - strand, within GFF1660 at 514.650 kb on + strand at 514.651 kb on - strand at 514.651 kb on - strand at 514.651 kb on - strand at 514.651 kb on - strand at 514.651 kb on - strand at 514.704 kb on + strand at 514.705 kb on - strand at 515.713 kb on + strand, within GFF1662 at 515.714 kb on - strand, within GFF1662 at 515.714 kb on - strand, within GFF1662 at 515.869 kb on + strand, within GFF1662 at 515.869 kb on + strand, within GFF1662 at 515.869 kb on + strand, within GFF1662 at 515.869 kb on + strand, within GFF1662 at 515.869 kb on + strand, within GFF1662 at 515.869 kb on + strand, within GFF1662 at 515.869 kb on + strand, within GFF1662 at 515.869 kb on + strand, within GFF1662 at 515.869 kb on + strand, within GFF1662 at 515.869 kb on + strand, within GFF1662 at 515.869 kb on + strand, within GFF1662 at 515.869 kb on + strand, within GFF1662 at 515.869 kb on + strand, within GFF1662 at 515.870 kb on - strand, within GFF1662 at 515.870 kb on - strand, within GFF1662 at 515.870 kb on - strand, within GFF1662 at 515.870 kb on - strand, within GFF1662 at 515.870 kb on - strand, within GFF1662 at 515.870 kb on - strand, within GFF1662 at 515.870 kb on - strand, within GFF1662 at 515.870 kb on - strand, within GFF1662 at 516.157 kb on + strand, within GFF1662 at 516.157 kb on + strand, within GFF1662 at 516.157 kb on + strand, within GFF1662 at 516.158 kb on - strand, within GFF1662 at 516.158 kb on - strand, within GFF1662 at 516.158 kb on - strand, within GFF1662 at 516.355 kb on + strand, within GFF1662 at 516.355 kb on + strand, within GFF1662 at 516.355 kb on + strand, within GFF1662 at 516.355 kb on + strand, within GFF1662 at 516.355 kb on + strand, within GFF1662 at 516.356 kb on - strand, within GFF1662 at 516.356 kb on - strand, within GFF1662 at 516.356 kb on - strand, within GFF1662
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 2 remove 514,260 + GFF1660 0.76 -0.3 514,260 + GFF1660 0.76 +0.3 514,260 + GFF1660 0.76 +0.2 514,261 - GFF1660 0.76 -0.2 514,311 + GFF1660 0.78 +0.8 514,311 + GFF1660 0.78 +0.2 514,311 + GFF1660 0.78 +0.8 514,312 - GFF1660 0.78 +0.3 514,344 + GFF1660 0.79 +1.0 514,488 + GFF1660 0.85 -0.1 514,489 - GFF1660 0.85 +0.1 514,489 - GFF1660 0.85 -0.3 514,489 - GFF1660 0.85 +0.2 514,489 - GFF1660 0.85 +0.0 514,492 - GFF1660 0.85 -1.3 514,650 + +0.9 514,651 - +0.1 514,651 - -0.1 514,651 - +0.2 514,651 - +0.1 514,651 - +0.5 514,704 + +0.1 514,705 - -0.4 515,713 + GFF1662 0.12 -0.4 515,714 - GFF1662 0.12 -0.0 515,714 - GFF1662 0.12 -0.0 515,869 + GFF1662 0.25 -1.8 515,869 + GFF1662 0.25 +0.4 515,869 + GFF1662 0.25 -1.1 515,869 + GFF1662 0.25 +0.7 515,869 + GFF1662 0.25 +0.9 515,869 + GFF1662 0.25 +0.3 515,869 + GFF1662 0.25 -1.0 515,869 + GFF1662 0.25 +1.3 515,869 + GFF1662 0.25 -0.2 515,869 + GFF1662 0.25 -0.0 515,869 + GFF1662 0.25 -0.2 515,869 + GFF1662 0.25 -0.1 515,869 + GFF1662 0.25 +0.2 515,870 - GFF1662 0.25 -1.6 515,870 - GFF1662 0.25 +0.0 515,870 - GFF1662 0.25 -0.2 515,870 - GFF1662 0.25 -0.0 515,870 - GFF1662 0.25 +0.2 515,870 - GFF1662 0.25 +0.2 515,870 - GFF1662 0.25 -0.0 515,870 - GFF1662 0.25 -0.0 516,157 + GFF1662 0.50 +0.6 516,157 + GFF1662 0.50 -1.1 516,157 + GFF1662 0.50 -0.0 516,158 - GFF1662 0.50 +0.8 516,158 - GFF1662 0.50 +1.2 516,158 - GFF1662 0.50 +0.9 516,355 + GFF1662 0.66 -0.8 516,355 + GFF1662 0.66 +0.1 516,355 + GFF1662 0.66 -0.1 516,355 + GFF1662 0.66 -0.4 516,355 + GFF1662 0.66 +0.0 516,356 - GFF1662 0.66 +1.2 516,356 - GFF1662 0.66 -0.4 516,356 - GFF1662 0.66 +0.2
Or see this region's nucleotide sequence