Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF1524

Experiment: Ying_Nucleobase14 rep A; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1522 and GFF1523 overlap by 4 nucleotidesGFF1523 and GFF1524 overlap by 4 nucleotidesGFF1524 and GFF1525 are separated by 26 nucleotides GFF1522 - Muconate cycloisomerase (EC 5.5.1.1), at 377,087 to 378,211 GFF1522 GFF1523 - decarboxylase, at 378,208 to 378,945 GFF1523 GFF1524 - putative exported protein, at 378,942 to 379,922 GFF1524 GFF1525 - Benzoylformate decarboxylase (EC 4.1.1.7), at 379,949 to 381,496 GFF1525 Position (kb) 378 379 380Strain fitness (log2 ratio) -1 0 1at 377.961 kb on + strand, within GFF1522at 378.707 kb on + strand, within GFF1523at 379.447 kb on + strand, within GFF1524at 379.447 kb on + strand, within GFF1524at 379.802 kb on - strand, within GFF1524at 380.235 kb on + strand, within GFF1525at 380.463 kb on + strand, within GFF1525at 380.464 kb on - strand, within GFF1525at 380.692 kb on - strand, within GFF1525at 380.692 kb on - strand, within GFF1525

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 2
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377,961 + GFF1522 0.78 -0.1
378,707 + GFF1523 0.68 +0.3
379,447 + GFF1524 0.51 +1.7
379,447 + GFF1524 0.51 +0.3
379,802 - GFF1524 0.88 +0.3
380,235 + GFF1525 0.18 +0.1
380,463 + GFF1525 0.33 -0.2
380,464 - GFF1525 0.33 -0.0
380,692 - GFF1525 0.48 +0.3
380,692 - GFF1525 0.48 +0.1

Or see this region's nucleotide sequence