Experiment: Ying_Nucleobase14 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF666 and GFF667 are separated by 41 nucleotides GFF667 and GFF1075 are separated by 144 nucleotides GFF1075 and GFF668 overlap by 10 nucleotides
GFF666 - Fused spore maturation proteins A and B, at 348,133 to 349,425
GFF666
GFF667 - Peptide methionine sulfoxide reductase MsrB (EC 1.8.4.12), at 349,467 to 349,940
GFF667
GFF1075 - tRNA-Arg-CCT, at 350,085 to 350,156
GFF1075
GFF668 - Transcriptional regulator, LysR family, at 350,147 to 351,118
GFF668
Position (kb) 350
351 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 349.297 kb on + strand at 349.297 kb on + strand at 349.298 kb on - strand at 349.298 kb on - strand at 349.298 kb on - strand at 349.298 kb on - strand at 349.298 kb on - strand at 349.298 kb on - strand at 349.298 kb on - strand at 349.312 kb on + strand at 349.312 kb on + strand at 349.312 kb on + strand at 349.312 kb on + strand at 349.312 kb on + strand at 349.313 kb on - strand at 349.313 kb on - strand at 349.409 kb on - strand at 349.513 kb on + strand at 349.513 kb on + strand at 349.513 kb on + strand at 349.514 kb on - strand at 349.768 kb on + strand, within GFF667 at 349.768 kb on + strand, within GFF667 at 349.768 kb on + strand, within GFF667 at 349.769 kb on - strand, within GFF667 at 349.977 kb on + strand at 349.978 kb on - strand at 349.978 kb on - strand at 349.978 kb on - strand at 350.018 kb on + strand at 350.019 kb on - strand at 350.019 kb on - strand at 350.019 kb on - strand at 350.019 kb on - strand at 350.109 kb on + strand, within GFF1075 at 350.109 kb on + strand, within GFF1075 at 350.110 kb on - strand, within GFF1075 at 350.110 kb on - strand, within GFF1075 at 350.110 kb on - strand, within GFF1075 at 350.114 kb on - strand, within GFF1075 at 350.114 kb on - strand, within GFF1075 at 350.121 kb on - strand, within GFF1075 at 350.148 kb on + strand at 350.148 kb on + strand at 350.149 kb on - strand at 350.238 kb on + strand at 350.238 kb on + strand at 350.238 kb on + strand at 350.238 kb on + strand at 350.239 kb on - strand at 350.239 kb on - strand at 350.385 kb on + strand, within GFF668 at 350.385 kb on + strand, within GFF668 at 350.385 kb on + strand, within GFF668 at 350.386 kb on - strand, within GFF668 at 350.386 kb on - strand, within GFF668 at 350.386 kb on - strand, within GFF668 at 350.458 kb on - strand, within GFF668 at 350.458 kb on - strand, within GFF668 at 350.778 kb on + strand, within GFF668 at 350.778 kb on + strand, within GFF668 at 350.778 kb on + strand, within GFF668 at 350.779 kb on - strand, within GFF668 at 350.779 kb on - strand, within GFF668 at 350.925 kb on + strand, within GFF668 at 350.925 kb on + strand, within GFF668 at 350.926 kb on - strand, within GFF668 at 351.154 kb on + strand at 351.154 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 2 remove 349,297 + -0.5 349,297 + +1.0 349,298 - +0.3 349,298 - -0.2 349,298 - -0.1 349,298 - -0.5 349,298 - -0.8 349,298 - -1.8 349,298 - +0.8 349,312 + -0.1 349,312 + +0.1 349,312 + +1.4 349,312 + +0.2 349,312 + -0.8 349,313 - -0.0 349,313 - -0.3 349,409 - +0.2 349,513 + +0.1 349,513 + -0.0 349,513 + -0.0 349,514 - +0.7 349,768 + GFF667 0.64 -2.0 349,768 + GFF667 0.64 +1.0 349,768 + GFF667 0.64 +0.2 349,769 - GFF667 0.64 -1.5 349,977 + -0.5 349,978 - -0.3 349,978 - +0.3 349,978 - +1.3 350,018 + -0.1 350,019 - +0.2 350,019 - -0.1 350,019 - -0.5 350,019 - +0.9 350,109 + GFF1075 0.33 -4.0 350,109 + GFF1075 0.33 -1.6 350,110 - GFF1075 0.35 -0.3 350,110 - GFF1075 0.35 -1.9 350,110 - GFF1075 0.35 -2.2 350,114 - GFF1075 0.40 -3.2 350,114 - GFF1075 0.40 -0.5 350,121 - GFF1075 0.50 -2.6 350,148 + -2.3 350,148 + -2.0 350,149 - -1.3 350,238 + +0.3 350,238 + -0.7 350,238 + -0.6 350,238 + -0.2 350,239 - +0.2 350,239 - +0.5 350,385 + GFF668 0.24 -1.0 350,385 + GFF668 0.24 +1.5 350,385 + GFF668 0.24 -0.0 350,386 - GFF668 0.25 -0.4 350,386 - GFF668 0.25 -0.1 350,386 - GFF668 0.25 -0.6 350,458 - GFF668 0.32 -0.0 350,458 - GFF668 0.32 +0.5 350,778 + GFF668 0.65 -0.5 350,778 + GFF668 0.65 -0.9 350,778 + GFF668 0.65 +0.2 350,779 - GFF668 0.65 +0.3 350,779 - GFF668 0.65 +0.2 350,925 + GFF668 0.80 -0.0 350,925 + GFF668 0.80 +0.1 350,926 - GFF668 0.80 -1.3 351,154 + +1.1 351,154 + -0.4
Or see this region's nucleotide sequence