Experiment: Lentilan from mushroom 2.5 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BACOVA_02612 and BACOVA_02613 are separated by 76 nucleotides BACOVA_02613 and BACOVA_02614 overlap by 13 nucleotides BACOVA_02614 and BACOVA_02615 are separated by 216 nucleotides BACOVA_02615 and BACOVA_02616 are separated by 75 nucleotides
BACOVA_02612: BACOVA_02612 - DNA-binding helix-turn-helix protein, at 381,934 to 382,404
_02612
BACOVA_02613: BACOVA_02613 - oxidoreductase NAD-binding domain protein, at 382,481 to 383,257
_02613
BACOVA_02614: BACOVA_02614 - dihydroorotate dehydrogenase 1B, at 383,245 to 384,156
_02614
BACOVA_02615: BACOVA_02615 - tRNA (guanine-N(1)-)-methyltransferase, at 384,373 to 385,050
_02615
BACOVA_02616: BACOVA_02616 - DNA ligase (NAD+), at 385,126 to 387,126
_02616
Position (kb) 383
384
385 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 382.478 kb on + strand at 384.467 kb on - strand, within BACOVA_02615 at 384.495 kb on - strand, within BACOVA_02615 at 384.510 kb on + strand, within BACOVA_02615 at 384.511 kb on - strand, within BACOVA_02615 at 384.512 kb on + strand, within BACOVA_02615 at 384.513 kb on - strand, within BACOVA_02615 at 384.515 kb on - strand, within BACOVA_02615 at 384.515 kb on - strand, within BACOVA_02615 at 384.644 kb on - strand, within BACOVA_02615 at 384.644 kb on - strand, within BACOVA_02615 at 384.648 kb on - strand, within BACOVA_02615 at 384.708 kb on + strand, within BACOVA_02615 at 384.801 kb on + strand, within BACOVA_02615 at 384.852 kb on + strand, within BACOVA_02615 at 384.897 kb on - strand, within BACOVA_02615 at 384.953 kb on - strand, within BACOVA_02615 at 384.953 kb on - strand, within BACOVA_02615 at 385.036 kb on - strand at 385.090 kb on + strand at 385.125 kb on + strand at 385.125 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Lentilan from mushroom 2.5 mg/mL (C) remove 382,478 + -0.8 384,467 - BACOVA_02615 0.14 -2.6 384,495 - BACOVA_02615 0.18 -2.5 384,510 + BACOVA_02615 0.20 -1.1 384,511 - BACOVA_02615 0.20 -2.8 384,512 + BACOVA_02615 0.21 -1.1 384,513 - BACOVA_02615 0.21 -1.8 384,515 - BACOVA_02615 0.21 -1.3 384,515 - BACOVA_02615 0.21 -0.5 384,644 - BACOVA_02615 0.40 -1.5 384,644 - BACOVA_02615 0.40 -2.3 384,648 - BACOVA_02615 0.41 -0.7 384,708 + BACOVA_02615 0.49 -0.9 384,801 + BACOVA_02615 0.63 -1.1 384,852 + BACOVA_02615 0.71 -2.4 384,897 - BACOVA_02615 0.77 -1.1 384,953 - BACOVA_02615 0.86 -1.3 384,953 - BACOVA_02615 0.86 -0.8 385,036 - -1.7 385,090 + -2.2 385,125 + +0.4 385,125 + +0.3
Or see this region's nucleotide sequence