Experiment: Ying_OrganicAcid7 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3353 and GFF3354 are separated by 139 nucleotides GFF3354 and GFF3355 overlap by 4 nucleotides GFF3355 and GFF3356 are separated by 43 nucleotides GFF3356 and GFF3357 overlap by 4 nucleotides
GFF3353 - Acetyl-coenzyme A carboxyl transferase beta chain (EC 6.4.1.2), at 236,998 to 237,876
GFF3353
GFF3354 - Tryptophan synthase alpha chain (EC 4.2.1.20), at 238,016 to 238,825
GFF3354
GFF3355 - Tryptophan synthase beta chain (EC 4.2.1.20), at 238,822 to 240,093
GFF3355
GFF3356 - Phosphoribosylanthranilate isomerase (EC 5.3.1.24), at 240,137 to 240,817
GFF3356
GFF3357 - tRNA pseudouridine synthase A (EC 4.2.1.70), at 240,814 to 241,581
GFF3357
Position (kb) 238
239
240
241 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 238.017 kb on + strand at 238.017 kb on + strand at 238.287 kb on + strand, within GFF3354 at 238.290 kb on + strand, within GFF3354 at 238.290 kb on + strand, within GFF3354 at 238.290 kb on + strand, within GFF3354 at 238.291 kb on - strand, within GFF3354 at 238.291 kb on - strand, within GFF3354 at 238.296 kb on + strand, within GFF3354 at 238.296 kb on + strand, within GFF3354 at 238.297 kb on - strand, within GFF3354 at 238.500 kb on + strand, within GFF3354 at 238.501 kb on - strand, within GFF3354 at 238.501 kb on - strand, within GFF3354 at 238.521 kb on + strand, within GFF3354 at 238.521 kb on + strand, within GFF3354 at 238.522 kb on - strand, within GFF3354 at 238.522 kb on - strand, within GFF3354 at 238.762 kb on - strand at 239.327 kb on + strand, within GFF3355 at 239.519 kb on + strand, within GFF3355 at 239.519 kb on + strand, within GFF3355 at 239.520 kb on - strand, within GFF3355 at 239.637 kb on - strand, within GFF3355 at 240.044 kb on + strand at 240.044 kb on + strand at 240.080 kb on + strand at 240.081 kb on - strand at 240.335 kb on + strand, within GFF3356 at 240.335 kb on + strand, within GFF3356 at 240.335 kb on + strand, within GFF3356 at 240.335 kb on + strand, within GFF3356 at 240.393 kb on + strand, within GFF3356 at 240.393 kb on + strand, within GFF3356 at 240.394 kb on - strand, within GFF3356 at 240.451 kb on - strand, within GFF3356 at 240.456 kb on - strand, within GFF3356 at 240.712 kb on - strand, within GFF3356 at 240.712 kb on - strand, within GFF3356 at 240.712 kb on - strand, within GFF3356 at 240.869 kb on + strand at 240.869 kb on + strand at 240.869 kb on + strand at 240.869 kb on + strand at 240.869 kb on + strand at 240.887 kb on + strand at 241.058 kb on + strand, within GFF3357 at 241.059 kb on - strand, within GFF3357 at 241.059 kb on - strand, within GFF3357 at 241.059 kb on - strand, within GFF3357
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep C; time point 2 remove 238,017 + -0.1 238,017 + +0.2 238,287 + GFF3354 0.33 -1.4 238,290 + GFF3354 0.34 -3.6 238,290 + GFF3354 0.34 -4.8 238,290 + GFF3354 0.34 -5.4 238,291 - GFF3354 0.34 -3.5 238,291 - GFF3354 0.34 -2.8 238,296 + GFF3354 0.35 -1.5 238,296 + GFF3354 0.35 -0.9 238,297 - GFF3354 0.35 -3.3 238,500 + GFF3354 0.60 -3.1 238,501 - GFF3354 0.60 -4.2 238,501 - GFF3354 0.60 -4.7 238,521 + GFF3354 0.62 -5.3 238,521 + GFF3354 0.62 -2.2 238,522 - GFF3354 0.62 -1.4 238,522 - GFF3354 0.62 -0.7 238,762 - -4.7 239,327 + GFF3355 0.40 -1.6 239,519 + GFF3355 0.55 -3.4 239,519 + GFF3355 0.55 -1.2 239,520 - GFF3355 0.55 -2.4 239,637 - GFF3355 0.64 -2.3 240,044 + -1.6 240,044 + -2.1 240,080 + -1.2 240,081 - -3.1 240,335 + GFF3356 0.29 -1.6 240,335 + GFF3356 0.29 -1.8 240,335 + GFF3356 0.29 -1.9 240,335 + GFF3356 0.29 -3.6 240,393 + GFF3356 0.38 -0.9 240,393 + GFF3356 0.38 -2.1 240,394 - GFF3356 0.38 -4.1 240,451 - GFF3356 0.46 -2.1 240,456 - GFF3356 0.47 -1.2 240,712 - GFF3356 0.84 -0.9 240,712 - GFF3356 0.84 -1.2 240,712 - GFF3356 0.84 -2.1 240,869 + -1.6 240,869 + -0.8 240,869 + -0.7 240,869 + +0.4 240,869 + +0.2 240,887 + +1.7 241,058 + GFF3357 0.32 -0.4 241,059 - GFF3357 0.32 +0.2 241,059 - GFF3357 0.32 -0.1 241,059 - GFF3357 0.32 +0.7
Or see this region's nucleotide sequence