Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF3143

Experiment: Ying_OrganicAcid7 rep C; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3141 and GFF3142 are separated by 215 nucleotidesGFF3142 and GFF3143 overlap by 4 nucleotidesGFF3143 and GFF3144 overlap by 4 nucleotides GFF3141 - hypothetical protein, at 19,880 to 20,110 GFF3141 GFF3142 - Conjugative signal peptidase TrhF, at 20,326 to 20,937 GFF3142 GFF3143 - hypothetical protein, at 20,934 to 21,296 GFF3143 GFF3144 - IncF plasmid conjugative transfer pilus assembly protein TraC, at 21,293 to 23,875 GFF3144 Position (kb) 20 21 22Strain fitness (log2 ratio) -1 0 1 2at 20.064 kb on + strand, within GFF3141at 20.065 kb on - strand, within GFF3141at 20.065 kb on - strand, within GFF3141at 20.426 kb on + strand, within GFF3142at 20.427 kb on - strand, within GFF3142at 20.429 kb on + strand, within GFF3142at 20.430 kb on - strand, within GFF3142at 20.801 kb on + strand, within GFF3142at 20.801 kb on + strand, within GFF3142at 21.468 kb on + strandat 21.468 kb on + strandat 21.469 kb on - strandat 21.688 kb on - strand, within GFF3144at 21.955 kb on - strand, within GFF3144at 21.973 kb on - strand, within GFF3144at 22.131 kb on + strand, within GFF3144at 22.270 kb on - strand, within GFF3144

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep C; time point 2
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20,064 + GFF3141 0.80 -1.2
20,065 - GFF3141 0.80 +0.4
20,065 - GFF3141 0.80 +1.6
20,426 + GFF3142 0.16 -0.6
20,427 - GFF3142 0.17 -0.2
20,429 + GFF3142 0.17 +0.3
20,430 - GFF3142 0.17 +2.6
20,801 + GFF3142 0.78 -0.2
20,801 + GFF3142 0.78 -0.8
21,468 + -0.1
21,468 + +0.0
21,469 - +0.3
21,688 - GFF3144 0.15 -0.3
21,955 - GFF3144 0.26 -0.1
21,973 - GFF3144 0.26 -0.1
22,131 + GFF3144 0.32 -0.5
22,270 - GFF3144 0.38 +0.5

Or see this region's nucleotide sequence