Experiment: Ying_OrganicAcid7 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5663 and GFF5664 overlap by 4 nucleotides GFF5664 and GFF5665 are separated by 11 nucleotides GFF5665 and GFF5666 are separated by 3 nucleotides
GFF5663 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,687,252 to 2,688,028
GFF5663
GFF5664 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,688,025 to 2,688,804
GFF5664
GFF5665 - Lipid carrier protein or keto acyl-CoA thiolase Ltp3 (EC 2.3.1.16), at 2,688,816 to 2,690,018
GFF5665
GFF5666 - Hydroxymethylglutaryl-CoA synthase (EC 2.3.3.10), at 2,690,022 to 2,691,500
GFF5666
Position (kb)
2688
2689
2690
2691 Strain fitness (log2 ratio)
-1
0
1 at 2688.078 kb on - strand at 2688.078 kb on - strand at 2688.946 kb on + strand, within GFF5665 at 2688.955 kb on + strand, within GFF5665 at 2688.955 kb on + strand, within GFF5665 at 2688.955 kb on + strand, within GFF5665 at 2689.127 kb on - strand, within GFF5665 at 2689.192 kb on + strand, within GFF5665 at 2689.228 kb on + strand, within GFF5665 at 2689.228 kb on + strand, within GFF5665 at 2689.229 kb on - strand, within GFF5665 at 2689.387 kb on - strand, within GFF5665 at 2689.388 kb on - strand, within GFF5665 at 2689.604 kb on - strand, within GFF5665 at 2689.619 kb on - strand, within GFF5665 at 2689.720 kb on + strand, within GFF5665 at 2690.023 kb on + strand at 2690.342 kb on - strand, within GFF5666 at 2690.518 kb on + strand, within GFF5666 at 2690.519 kb on - strand, within GFF5666
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 2 remove 2,688,078 - -1.4 2,688,078 - +0.4 2,688,946 + GFF5665 0.11 -0.1 2,688,955 + GFF5665 0.12 -1.1 2,688,955 + GFF5665 0.12 -0.6 2,688,955 + GFF5665 0.12 -0.6 2,689,127 - GFF5665 0.26 -0.6 2,689,192 + GFF5665 0.31 +0.2 2,689,228 + GFF5665 0.34 -0.4 2,689,228 + GFF5665 0.34 +0.2 2,689,229 - GFF5665 0.34 -0.6 2,689,387 - GFF5665 0.47 +0.4 2,689,388 - GFF5665 0.48 -0.1 2,689,604 - GFF5665 0.66 -0.7 2,689,619 - GFF5665 0.67 -0.0 2,689,720 + GFF5665 0.75 -1.7 2,690,023 + +1.1 2,690,342 - GFF5666 0.22 -1.5 2,690,518 + GFF5666 0.34 -0.4 2,690,519 - GFF5666 0.34 -0.1
Or see this region's nucleotide sequence