Experiment: Ying_OrganicAcid7 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3142 and GFF3143 overlap by 4 nucleotides GFF3143 and GFF3144 overlap by 4 nucleotides GFF3144 and GFF3145 are separated by 7 nucleotides GFF3145 and GFF3146 overlap by 4 nucleotides
GFF3142 - Conjugative signal peptidase TrhF, at 20,326 to 20,937
GFF3142
GFF3143 - hypothetical protein, at 20,934 to 21,296
GFF3143
GFF3144 - IncF plasmid conjugative transfer pilus assembly protein TraC, at 21,293 to 23,875
GFF3144
GFF3145 - Conjugative transfer protein TraV, at 23,883 to 24,620
GFF3145
GFF3146 - Thiol:disulfide involved in conjugative transfer, at 24,617 to 25,486
GFF3146
Position (kb) 21
22
23
24 Strain fitness (log2 ratio) -1
0
1 at 20.426 kb on + strand, within GFF3142 at 20.427 kb on - strand, within GFF3142 at 20.429 kb on + strand, within GFF3142 at 20.430 kb on - strand, within GFF3142 at 20.801 kb on + strand, within GFF3142 at 20.801 kb on + strand, within GFF3142 at 21.468 kb on + strand at 21.468 kb on + strand at 21.469 kb on - strand at 21.688 kb on - strand, within GFF3144 at 21.955 kb on - strand, within GFF3144 at 21.973 kb on - strand, within GFF3144 at 22.131 kb on + strand, within GFF3144 at 22.270 kb on - strand, within GFF3144 at 22.350 kb on + strand, within GFF3144 at 22.485 kb on - strand, within GFF3144 at 22.648 kb on - strand, within GFF3144 at 22.648 kb on - strand, within GFF3144 at 22.752 kb on + strand, within GFF3144 at 23.754 kb on + strand at 23.764 kb on - strand at 24.130 kb on + strand, within GFF3145 at 24.137 kb on - strand, within GFF3145 at 24.137 kb on - strand, within GFF3145 at 24.413 kb on - strand, within GFF3145 at 24.413 kb on - strand, within GFF3145 at 24.413 kb on - strand, within GFF3145 at 24.514 kb on - strand, within GFF3145 at 24.564 kb on - strand at 24.842 kb on + strand, within GFF3146
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 2 remove 20,426 + GFF3142 0.16 +1.3 20,427 - GFF3142 0.17 -1.1 20,429 + GFF3142 0.17 +0.1 20,430 - GFF3142 0.17 +1.0 20,801 + GFF3142 0.78 -0.3 20,801 + GFF3142 0.78 +0.9 21,468 + -0.0 21,468 + -0.2 21,469 - +0.3 21,688 - GFF3144 0.15 -0.4 21,955 - GFF3144 0.26 +0.1 21,973 - GFF3144 0.26 +0.2 22,131 + GFF3144 0.32 +0.0 22,270 - GFF3144 0.38 +0.3 22,350 + GFF3144 0.41 -0.1 22,485 - GFF3144 0.46 +1.2 22,648 - GFF3144 0.52 +0.4 22,648 - GFF3144 0.52 +0.4 22,752 + GFF3144 0.56 +0.2 23,754 + +0.3 23,764 - -0.0 24,130 + GFF3145 0.33 +0.6 24,137 - GFF3145 0.34 +0.0 24,137 - GFF3145 0.34 -0.8 24,413 - GFF3145 0.72 +0.0 24,413 - GFF3145 0.72 +0.8 24,413 - GFF3145 0.72 -0.2 24,514 - GFF3145 0.86 -0.5 24,564 - +0.4 24,842 + GFF3146 0.26 -1.0
Or see this region's nucleotide sequence