Experiment: Ying_OrganicAcid7 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF502 and GFF503 are separated by 13 nucleotides GFF503 and GFF504 are separated by 93 nucleotides GFF504 and GFF505 are separated by 44 nucleotides
GFF502 - Type III secretion inner membrane protein (YscU,SpaS,EscU,HrcU,SsaU, homologous to flagellar export components), at 169,598 to 170,650
GFF502
GFF503 - PROBABLE TRANSMEMBRANE PROTEIN, at 170,664 to 171,071
GFF503
GFF504 - Branched-chain amino acid ABC transporter, amino acid-binding protein (TC 3.A.1.4.1), at 171,165 to 172,331
GFF504
GFF505 - Branched-chain amino acid ABC transporter, amino acid-binding protein (TC 3.A.1.4.1), at 172,376 to 173,542
GFF505
Position (kb)
171
172
173 Strain fitness (log2 ratio)
-1
0
1 at 170.408 kb on + strand, within GFF502 at 170.765 kb on - strand, within GFF503 at 170.807 kb on - strand, within GFF503 at 171.184 kb on + strand at 171.184 kb on + strand at 171.185 kb on - strand at 171.395 kb on - strand, within GFF504 at 171.401 kb on - strand, within GFF504 at 171.401 kb on - strand, within GFF504 at 171.515 kb on - strand, within GFF504 at 171.621 kb on - strand, within GFF504 at 171.725 kb on - strand, within GFF504 at 171.883 kb on + strand, within GFF504 at 171.883 kb on + strand, within GFF504 at 171.886 kb on + strand, within GFF504 at 171.928 kb on + strand, within GFF504 at 171.929 kb on - strand, within GFF504 at 172.094 kb on - strand, within GFF504 at 172.094 kb on - strand, within GFF504 at 172.094 kb on - strand, within GFF504 at 172.692 kb on + strand, within GFF505 at 172.719 kb on + strand, within GFF505 at 172.719 kb on + strand, within GFF505 at 172.768 kb on - strand, within GFF505 at 172.912 kb on - strand, within GFF505 at 173.133 kb on + strand, within GFF505 at 173.134 kb on - strand, within GFF505 at 173.134 kb on - strand, within GFF505 at 173.134 kb on - strand, within GFF505 at 173.134 kb on - strand, within GFF505 at 173.298 kb on + strand, within GFF505 at 173.299 kb on - strand, within GFF505
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep A; time point 2 remove 170,408 + GFF502 0.77 +0.3 170,765 - GFF503 0.25 +1.3 170,807 - GFF503 0.35 +1.1 171,184 + -0.7 171,184 + +1.1 171,185 - -0.0 171,395 - GFF504 0.20 +0.6 171,401 - GFF504 0.20 +0.4 171,401 - GFF504 0.20 -0.6 171,515 - GFF504 0.30 +0.8 171,621 - GFF504 0.39 -0.2 171,725 - GFF504 0.48 +0.9 171,883 + GFF504 0.62 +0.3 171,883 + GFF504 0.62 +0.1 171,886 + GFF504 0.62 +0.2 171,928 + GFF504 0.65 -0.1 171,929 - GFF504 0.65 -0.1 172,094 - GFF504 0.80 -0.2 172,094 - GFF504 0.80 +0.4 172,094 - GFF504 0.80 -0.1 172,692 + GFF505 0.27 +0.5 172,719 + GFF505 0.29 +0.3 172,719 + GFF505 0.29 +0.8 172,768 - GFF505 0.34 +0.5 172,912 - GFF505 0.46 +0.1 173,133 + GFF505 0.65 -0.2 173,134 - GFF505 0.65 -0.1 173,134 - GFF505 0.65 +0.0 173,134 - GFF505 0.65 +0.1 173,134 - GFF505 0.65 -0.3 173,298 + GFF505 0.79 -0.8 173,299 - GFF505 0.79 -0.8
Or see this region's nucleotide sequence